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Information on EC 2.7.8.41 - cardiolipin synthase (CMP-forming) and Organism(s) Arabidopsis thaliana and UniProt Accession Q93YW7

for references in articles please use BRENDA:EC2.7.8.41
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IUBMB Comments
The eukaryotic enzyme is involved in the biosynthesis of the mitochondrial phospholipid cardiolipin. It requires divalent cations for activity.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q93YW7
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
hcls1, crls1, cls_cap, cls1p, cardiolipin synthase 1, crcls1, cls 1, sco1389, ydl142c, cardiolipin synthase-1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:L-1-phosphatidyl-glycerol diacylglycerolphosphotransferase (CMP-forming)
The eukaryotic enzyme is involved in the biosynthesis of the mitochondrial phospholipid cardiolipin. It requires divalent cations for activity.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-dioleoylglycerol + dioleoylphosphatidylglycerol
a cardiolipin + CMP
show the reaction diagram
the enzyme is less active with phosphatidyl-glycerol species carrying one or two palmitoyl groups than with the dioleoyl species of phosphatidyl-glycerol. In addition, CDP-dipalmitoylglycerol hardly competes with CDP-dioleolyglycerol as substrate
-
-
?
CDP-dioleoylglycerol + dipalmitoylphosphatidylglycerol
a cardiolipin + CMP
show the reaction diagram
the enzyme is less active with phosphatidyl-glycerol species carrying one or two palmitoyl groups than with the dioleoyl species of phosphatidyl-glycerol. In addition, CDP-dipalmitoylglycerol hardly competes with CDP-dioleolyglycerol as substrate
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CoCl2
requires divalent cations for activity. Highest activities are measured in the presence of 20 mM MnCl2, while MgCl2 or CoCl2 stimulate the activity only slightly
MgCl2
requires divalent cations for activity. Highest activities are measured in the presence of 20 mM MnCl2, while MgCl2 or CoCl2 stimulate the activity only slightly
MnCl2
requires divalent cations for activity. Highest activities are measured in the presence of 20 mM MnCl2, while MgCl2 or CoCl2 stimulate the activity only slightly
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
0.2 mM, 50% inhibition. Concentrations higher than 0.6 mM result in an almost complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014
CDP-dioleoylglycerol
pH 8.8, 30°C
0.15
dioleoylphosphatidylglycerol
pH 8.8, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
in contrast to yeast, where development of deletion mutants is little affected, the Arabidopsis seedlings are slow developing under short-day conditions in vitro and die if they are transferred to long-day conditions
physiological function
-
the enzyme is crucial for correct mitochondrial function and development in Arabidopsis under both optimal and stress conditions
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CLS_ARATH
341
1
38042
Swiss-Prot
Mitochondrion (Reliability: 3)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
the preprotein expressed in yeast can be imported into mitochondria and processed to a catalytically active protein
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nowicki, M.; Mller, F.; Frentzen, M.
Cardiolipin synthase of Arabidopsis thaliana
FEBS Lett.
579
2161-2165
2005
Arabidopsis thaliana (Q93YW7), Arabidopsis thaliana
Manually annotated by BRENDA team
Pineau, B.; Bourge, M.; Marion, J.; Mauve, C.; Gilard, F.; Maneta-Peyret, L.; Moreau, P.; Satiat-Jeunemaitre, B.; Brown, S.C.; De Paepe, R.; Danon, A.
The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Plant Cell
25
4195-4208
2013
Arabidopsis thaliana
Manually annotated by BRENDA team