Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.2 mM, 50% inhibition. Concentrations higher than 0.6 mM result in an almost complete inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
CDP-dioleoylglycerol | pH 8.8, 30°C | Arabidopsis thaliana | |
0.15 | - |
dioleoylphosphatidylglycerol | pH 8.8, 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | requires divalent cations for activity. Highest activities are measured in the presence of 20 mM MnCl2, while MgCl2 or CoCl2 stimulate the activity only slightly | Arabidopsis thaliana | |
MgCl2 | requires divalent cations for activity. Highest activities are measured in the presence of 20 mM MnCl2, while MgCl2 or CoCl2 stimulate the activity only slightly | Arabidopsis thaliana | |
MnCl2 | requires divalent cations for activity. Highest activities are measured in the presence of 20 mM MnCl2, while MgCl2 or CoCl2 stimulate the activity only slightly | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93YW7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the preprotein expressed in yeast can be imported into mitochondria and processed to a catalytically active protein | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-dioleoylglycerol + dioleoylphosphatidylglycerol | the enzyme is less active with phosphatidyl-glycerol species carrying one or two palmitoyl groups than with the dioleoyl species of phosphatidyl-glycerol. In addition, CDP-dipalmitoylglycerol hardly competes with CDP-dioleolyglycerol as substrate | Arabidopsis thaliana | a cardiolipin + CMP | - |
? | |
CDP-dioleoylglycerol + dipalmitoylphosphatidylglycerol | the enzyme is less active with phosphatidyl-glycerol species carrying one or two palmitoyl groups than with the dioleoyl species of phosphatidyl-glycerol. In addition, CDP-dipalmitoylglycerol hardly competes with CDP-dioleolyglycerol as substrate | Arabidopsis thaliana | a cardiolipin + CMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At4g04870 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Arabidopsis thaliana |
9 | - |
- |
Arabidopsis thaliana |