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Information on EC 2.7.1.1 - hexokinase and Organism(s) Solanum tuberosum and UniProt Accession O64390

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IUBMB Comments
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
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This record set is specific for:
Solanum tuberosum
UNIPROT: O64390
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The taxonomic range for the selected organisms is: Solanum tuberosum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hexokinase, hexokinase ii, hexokinase 2, hexokinase i, hk ii, hxk, liver glucokinase, hexokinase 1, hexokinase-2, hkdc1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP-D-hexose 6-phosphotransferase
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-
-
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ATP-dependent hexokinase
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-
-
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brain form hexokinase
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-
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glucokinase
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glucose ATP phosphotransferase
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-
-
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hexokinase (phosphorylating)
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-
-
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hexokinase 1
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hexokinase 2
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hexokinase D
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-
-
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hexokinase PI
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-
-
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hexokinase PII
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-
-
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hexokinase type IV
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-
-
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hexokinase type IV glucokinase
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-
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hexokinase, tumor isozyme
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-
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HK
-
-
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HK4
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-
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HXK
-
-
-
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kinase, hexo- (phosphorylating)
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-
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muscle form hexokinase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-hexose 6-phosphotransferase
D-Glucose, D-mannose, D-fructose, sorbitol and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-51-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
?
D-fructose + ATP
ADP + D-fructose 6-phosphate
show the reaction diagram
D-glucose + ATP
ADP + D-glucose 6-phosphate
show the reaction diagram
D-glucose + ATP
D-glucose 6-phosphate + ADP
show the reaction diagram
D-mannose + ATP
ADP + D-mannose 6-phosphate
show the reaction diagram
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HK1: 53% of the activity with glucose, HK2: 22% of the activity with glucose, HK3: 45% of the activity with glucose
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-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-glucose
UDP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucose + ATP
D-glucose 6-phosphate + ADP
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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Mg2+-dependent
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucose 6-phosphate
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D-glucose 6-phosphate
UMP
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very weak
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09 - 0.56
ATP
8.7 - 22
D-fructose
0.035 - 0.13
D-glucose
0.038 - 0.29
D-mannose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04 - 0.11
ADP
4 - 4.1
D-glucose 6-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.06
-
HK3, at pH 8
0.72
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HK2, at pH 8
0.84
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HK1, at pH 8
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 8.2
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HK 1
7.5 - 9
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HK 2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 9
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pH 6.7: about 40% of activity maximum, pH 9: about 70% of activity maximum, isoenzyme HK1
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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transgenic Solanum tuberosum roots are altered in their hexokinase activity by transformation with an hexokinase cDNA in sense or antisense orientation. Altering root hexokinase activity levels impacts on growth rate and hexokinase has a high flux control coefficient over glucose phosphorylation but does not control glycolytic flux or respiration rate. It is concluded that futile cycling of hexose-phosphate can be partially responsible for the differences in energetic status in roots with high and low hexokinase activity and possibly cause the observed alterations in growth in transgenic roots
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HXK1_SOLTU
498
1
54130
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51000
-
SDS-PAGE
66000
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HK1 and HK2, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-180°C, 2 months, stable
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 isoenzymes: HK1: 140fold, HK2: 40fold, HK3: 25fold
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using hydrophobic-interaction chromatography on a butyl-sepharose™ 4 Fast Flow column. Main hexokinase isoform is purified to homogeneity using further chromatographic separations on red dye, DEAE Fractogel, hydroxyapatite, cibacron blue, and MonoQ matrices
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Renz, A.; Merlo, L.; Stitt, M.
Partial purification from potato tubers of three fructokinases and three hexokinases which show differing organ and developmental specificity
Planta
190
156-165
1993
Solanum tuberosum
-
Manually annotated by BRENDA team
Renz, A.; Stitt, M.
Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubers
Planta
190
166-175
1993
Solanum tuberosum
-
Manually annotated by BRENDA team
Claeyssen, E.; Rivoal, J.
Isozymes of plant hexokinase: occurrence, properties and functions
Phytochemistry
68
709-731
2007
Saccharomyces cerevisiae, Solanum lycopersicum, Pisum sativum, Solanum tuberosum, Solanum tuberosum (O64390), Solanum tuberosum (Q9SQ76), Triticum aestivum, Zea mays, Solanum chacoense, Capsicum chacoense, Arabidopsis thaliana (Q9FZG4), Arabidopsis thaliana
Manually annotated by BRENDA team
Moisan, M.; Rivoal, J.
Purification to homogeneity and characterization of nonproteolyzed potato (Solanum tuberosum) tuber hexokinase 1
Botany
89
289-299
2011
Solanum tuberosum
-
Manually annotated by BRENDA team
Claeyssen, E.; Dorion, S.; Clendenning, A.; He, J.Z.; Wally, O.; Chen, J.; Auslender, E.L.; Moisan, M.C.; Jolicoeur, M.; Rivoal, J.
The futile cycling of hexose phosphates could account for the fact that hexokinase exerts a high control on glucose phosphorylation but not on glycolytic rate in transgenic potato (Solanum tuberosum) roots
PLoS ONE
8
e53898
2013
Solanum tuberosum
Manually annotated by BRENDA team