Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.6.1.19 - 4-aminobutyrate-2-oxoglutarate transaminase and Organism(s) Mus musculus and UniProt Accession P61922

for references in articles please use BRENDA:EC2.6.1.19
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.19 4-aminobutyrate-2-oxoglutarate transaminase
IUBMB Comments
Requires pyridoxal phosphate. Some preparations also act on beta-alanine, 5-aminopentanoate and (R,S)-3-amino-2-methylpropanoate. cf. EC 2.6.1.120, beta-alanine---2-oxoglutarate transaminase.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
UNIPROT: P61922
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
gaba-t, gaba transaminase, gaba-transaminase, gaba-at, 4-aminobutyrate aminotransferase, gaba aminotransferase, gamma-aminobutyric acid transaminase, gabat, gamma-aminobutyric acid aminotransferase, gamma-aminobutyrate transaminase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4-aminobutyrate aminotransferase
-
-
-
-
4-aminobutyrate-2-ketoglutarate aminotransferase
-
-
-
-
4-aminobutyrate-2-oxoglutarate aminotransferase
-
-
-
-
4-aminobutyrate-2-oxoglutarate transaminase
-
-
-
-
4-aminobutyric acid 2-ketoglutaric acid aminotransferase
-
-
-
-
4-aminobutyric acid aminotransferase
-
-
-
-
aminobutyrate aminotransferase
-
-
-
-
aminobutyrate transaminase
-
-
-
-
aminotransferase, aminobutyrate
-
-
-
-
beta-alanine aminotransferase
-
-
-
-
beta-alanine transaminase
-
-
-
-
beta-alanine-oxoglutarate aminotransferase
-
-
-
-
beta-alanine-oxoglutarate transaminase
-
-
-
-
GABA aminotransferase
-
-
-
-
GABA transaminase
-
-
-
-
GABA transferase
-
-
-
-
GABA-2-oxoglutarate aminotransferase
-
-
-
-
GABA-2-oxoglutarate transaminase
-
-
-
-
GABA-alpha-ketoglutarate aminotransferase
-
-
-
-
GABA-alpha-ketoglutarate transaminase
-
-
-
-
GABA-alpha-ketoglutaric acid transaminase
-
-
-
-
GABA-alpha-oxoglutarate aminotransferase
-
-
-
-
GABA-oxoglutarate aminotransferase
-
-
-
-
GABA-oxoglutarate transaminase
-
-
-
-
GABA-T
-
-
gamma-aminobutyrate aminotransaminase
-
-
-
-
gamma-aminobutyrate transaminase
-
-
-
-
gamma-aminobutyrate-alpha-ketoglutarate aminotransferase
-
-
-
-
gamma-aminobutyrate-alpha-ketoglutarate transaminase
-
-
-
-
gamma-aminobutyrate:alpha-oxoglutarate aminotransferase
-
-
-
-
gamma-aminobutyric acid aminotransferase
-
-
-
-
gamma-aminobutyric acid pyruvate transaminase
-
-
-
-
gamma-aminobutyric acid transaminase
-
-
-
-
gamma-aminobutyric acid-2-oxoglutarate transaminase
-
-
-
-
gamma-aminobutyric acid-alpha-ketoglutarate transaminase
-
-
-
-
gamma-aminobutyric acid-alpha-ketoglutaric acid aminotransferase
-
-
-
-
gamma-aminobutyric transaminase
-
-
-
-
glutamate-succinic semialdehyde transaminase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
show the reaction diagram
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
4-aminobutanoate:2-oxoglutarate aminotransferase
Requires pyridoxal phosphate. Some preparations also act on beta-alanine, 5-aminopentanoate and (R,S)-3-amino-2-methylpropanoate. cf. EC 2.6.1.120, beta-alanine---2-oxoglutarate transaminase.
CAS REGISTRY NUMBER
COMMENTARY hide
9037-67-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-aminoisobutanoate + 2-oxoglutarate
L-glutamate + 3-oxoisobutanoate
show the reaction diagram
4-aminobutanoate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
5-aminopentanoate + 2-oxoglutarate
L-glutamate + 5-oxopentanoate
show the reaction diagram
6-aminohexanoate + 2-oxoglutarate
L-glutamate + 6-oxohexanoate
show the reaction diagram
-
-
-
-
?
beta-alanine + 2-oxoglutarate
malonic semialdehyde + L-glutamate
show the reaction diagram
DL-3-hydroxy-4-aminobutanoate + 2-oxoglutarate
L-glutamate + 3-hydroxy-4-oxobutanoate
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-aminobutanoate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(4-acetylphenyl)-3-(4-bromophenyloxy)-pyrrolidine-2,5-dione
-
1-(4-acetylphenyl)-3-(salicyldehydroxy)-pyrrolidine-2,5-dione
-
beta-cypermethrin
GABA transaminase activity detected is significantly decreased in the cerebral cortex of mice 2 h after beta-cypermethrin administration. beta-Cypermethrin (80 mg/kg) significantly increases GABA levels in the cerebral cortex of mice, at both 2 and 4 h after treatment, compared with the control. The number of positive granules is increased in the cerebral cortex of mice 4 h after exposure to 80 mg/kg beta-cypermethrin. No significant changes are found in glutamate decarboxylase activity, or the expression of GABA transaminase protein and GABAB receptors mRNA, in the cerebral cortex of mice, except that 80 mg/kg beta-cypermethrin causes a significant decrease in GABAA receptors mRNA expression 4 h after administration
2-oxoadipic acid
-
-
3-Chloro-4-aminobutanoate
-
-
3-Mercaptopropionic acid
-
-
3-Phenyl-4-aminobutanoate
-
-
6-Azathymine
-
-
6-Azauracil
-
63% inhibition at 1 mM, reversible by dialysis, not by pyridoxal phosphate addition
acetic acid
-
-
adipic acid
-
-
ADP
-
reversible
Aminooxyacetate
-
-
ATP
-
reversible
Ba2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Butyric acid
-
-
Ca2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Cd2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Co2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Cu2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
dioxan
-
5% v/v
Divalent metal ions
-
with decreasing efficiency: Hg2+, Cd2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
-
ethanol
-
10% v/v, weak
gabaculine
gamma-vinyl 4-aminobutanoate
-
0.1 mM, complete inhibition
GDP
-
reversible
glutamic acid
-
-
HgCl2
-
strong, 50% inhibition at 0.007 mM
hydrazine
-
-
hydroxylamine
-
-
methanol
-
10% v/v, weak
Mg2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Mn2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Ni2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
p-chloromercuribenzoate
-
strong
pimelic acid
-
-
propionic acid
-
-
SH-group reagents
-
-
-
Sr2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
vigabatrin
-
triggers a massive synaptic plasticity in retinal areas showing a normal layering of the retina shown by the withdrawal of rod but not cone photoreceptor terminals from the outer plexiform layers towards their cell bodies. Both rod bipolar cells and horizontal cells exhibit dendritic sprouting into the photoreceptor nuclear layer. Withdrawing rod photoreceptors appear to form ectopic contacts with growing postsynaptic dendrites. Neuronal plasticity is highly suggestive of an impaired glutamate release by photoreceptors
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13 - 0.27
2-oxoglutarate
-
37°C, pH 8.0
1.1
4-aminobutanoate
-
37°C, pH 8.0
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
3-Mercaptopropionic acid
-
-
3.7
ATP
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
1-(4-acetylphenyl)-3-(4-bromophenyloxy)-pyrrolidine-2,5-dione
Mus musculus
pH 7.5, 37°C
0.16
1-(4-acetylphenyl)-3-(salicyldehydroxy)-pyrrolidine-2,5-dione
Mus musculus
pH 7.5, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
37°C, pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
mitochondrial enzyme
8
-
synaptosomal enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 8.8
-
about half-maximal activity at pH 6.9 and 8.8
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
retinal tissue of albino mice treated with two vigabatrin doses
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GABT_MOUSE
500
0
56452
Swiss-Prot
Mitochondrion (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
109000
-
high speed sedimentation equilibrium centrifugation
53000
-
1 * 53000 + 1 * 58000, SDS-PAGE
58000
-
1 * 53000 + 1 * 58000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
1 * 53000 + 1 * 58000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freeze-thawing slightly inactivates
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, protected with 2-aminoethylisothiouronium bromide hydrobromide and pyridoxal phosphate, several months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schousboe, A.; Wu, J.Y.; Roberts, E.
Purification and characterization of the 4-aminobutyrate--2,ketoglutarate transaminase from mouse brain
Biochemistry
12
2868-2873
1973
Mus musculus
Manually annotated by BRENDA team
Tunnicliff, G.; Youngs, T.L.
Competitive inhibition of mouse brain gamma-aminobutyrate aminotransferase by ATP
Proc. Soc. Exp. Biol. Med.
192
11-15
1989
Mus musculus
Manually annotated by BRENDA team
Schousboe, A.; Wu, J.Y.; Roberts, E.
Subunit structure and kinetic properties of 4-aminobutyrate-2-ketoglutarate transaminase purified from mouse brain
J. Neurochem.
23
1189-1195
1974
Mus musculus
Manually annotated by BRENDA team
Buu, N.T.; van Gelder, N.M.
Differences in biochemical properties of gamma-aminobutyric acid aminotransferase from synaptosome-enriched and cytoplasmic mitochondria-enriched subcellular fractions of mouse brain
Can. J. Physiol. Pharmacol.
52
674-680
1974
Mus musculus
Manually annotated by BRENDA team
Cooper, A.J.L.
Glutamate-gamma-aminobutyrate transaminase
Methods Enzymol.
113
80-82
1985
Oryctolagus cuniculus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Rando, R.R.; Bangerter, F.W.
The irreversible inhibition of mouse brain gamma-aminobutyric acid (GABA)-alpha-ketoglutaric acid transaminase by gabaculine
J. Am. Chem. Soc.
98
6762-6764
1976
Mus musculus
Manually annotated by BRENDA team
Sonnewald, U.; Kortner, T.M.; Qu, H.; Olstad, E.; Sunol, C.; Bak, L.K.; Schousboe, A.; Waagepetersen, H.S.
Demonstration of extensive GABA synthesis in the small population of GAD positive neurons in cerebellar cultures by the use of pharmacological tools
Neurochem. Int.
48
572-578
2006
Mus musculus
Manually annotated by BRENDA team
Wang, Q.P.; Jammoul, F.; Duboc, A.; Gong, J.; Simonutti, M.; Dubus, E.; Craft, C.M.; Ye, W.; Sahel, J.A.; Picaud, S.
Treatment of epilepsy: the GABA-transaminase inhibitor, vigabatrin, induces neuronal plasticity in the mouse retina
Eur. J. Neurosci.
27
2177-2187
2008
Mus musculus
Manually annotated by BRENDA team
Han, Y.; Cao, D.; Li, X.; Zhang, R.; Yu, F.; Ren, Y.; An, L.
Attenuation of gamma-aminobutyric acid (GABA) transaminase activity contributes to GABA increase in the cerebral cortex of mice exposed to beta-cypermethrin
Hum. Exp. Toxicol.
33
317-324
2014
Mus musculus (P61922)
Manually annotated by BRENDA team
Patel, J.; Dholakiya, B.; Mishra, N.
In vitro brain GABA-transaminase activity of 1-(4-acetylphenyl)-3-aryloxypyrrolidine-2,5-dione derivatives
J. Pharm. Res.
6
442-446
2013
Mus musculus (P61922)
-
Manually annotated by BRENDA team