Any feedback?
Information on EC 2.4.1.362 - alpha-(1->3) branching sucrase
for references in articles please use BRENDA:EC2.4.1.362Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.4.1.362 alpha-(1->3) branching sucraseIUBMB Comments
The enzyme from Leuconostoc spp. is responsible for producing alpha-(1->3) branches in alpha-(1->6) glucans by transferring the glucose residue from fructose to a 3-hydroxyl group of a glucan.
Specify your search results
Word Map
- 2.4.1.362
- platelet
- dextrans
-
ringer
- bicarbonate
-
transfusions
-
glucosylation
-
allergic
-
apheresis
-
transfused
-
hypotonic
-
processor
- leuconostoc
-
acid-citrate-dextrose
-
comb-like
-
resuspended
-
gorge
- gluco-oligosaccharides
- citreum
-
volume-reduced
- glucansucrases
- synthesis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
brs-a, branching sucrase, brs-b, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
branching sucrase
branching sucrase A
-
-
-
-
branching sucrase B
BRS-A
-
-
-
-
BRS-B
BRS-BDELTA1
brsA
-
-
-
-
BrsB
GtfZ
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sucrose + a (1->6)-alpha-D-glucan = D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
sucrose:(1->6)-alpha-D-glucan 3-alpha-D-[(1->3)-alpha-D-glucosyl]-transferase
The enzyme from Leuconostoc spp. is responsible for producing alpha-(1->3) branches in alpha-(1->6) glucans by transferring the glucose residue from fructose to a 3-hydroxyl group of a glucan.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
isomalto/malto-polysaccharide + sucrose
D-fructose + isomalto/malto-polysaccharide containing a (1->3)-alpha-D-glucose branch
-
alpha(1->3)-branched IMMP, IMMP Mw is 70 kDa, transglucosylation activity
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
dextran + sucrose
D-fructose + dextran containing a (1->3)-alpha-D-glucose branch
-
dextran Mw is 70 kDa, transglucosylation activity
-
-
?
dextran + sucrose
D-fructose + dextran containing a (1->3)-alpha-D-glucose branch
-
dextran Mw is 70 kDa, transglucosylation activity
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
transglucosylation activity
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
transglucosylation activity
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
Leuconostoc citreum NRRL B-1299
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
dextran of 70 kDa
-
-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
dextran of 70 kDa
-
-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
dextran of 70 kDa
-
-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
dextran of 70 kDa
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
sucrose hydrolysis
-
-
?
additional information
?
-
-
domain CD2 additionally displays hydrolytic acivity on dextran
-
-
?
additional information
?
-
-
enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products
-
-
?
additional information
?
-
-
enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages)
-
-
-
additional information
?
-
-
domain CD2 additionally displays hydrolytic acivity on dextran
-
-
?
additional information
?
-
-
enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products
-
-
?
additional information
?
-
-
enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages)
-
-
-
additional information
?
-
-
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages
-
-
-
additional information
?
-
Leuconostoc citreum NRRL B-1299
-
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages
-
-
-
additional information
?
-
-
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
Leuconostoc citreum NRRL B-1299
-
-
-
-
?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Dextran
-
presence of dextran highly stimulates the activity of domain CD2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Transfusion Reaction
Investigation of factors associated with allergic transfusion reaction due to platelet transfusion and the efficacy of platelets resuspended in BRS-A in adult patients.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
physiological function
additional information
evolution
-
a distict subgroup of sucrose-active enzymes is included in the GH70 family, corresponding to enzymes that display no polymerase activity, but efficiently transfer the glucosyl residue from sucrose to linear alpha(1->6) dextrans through the specific formation of single alpha(1->2) or alpha(1->3) linked glucosyl units. They are named branching sucrases (BRSs)
evolution
-
a distict subgroup of sucrose-active enzymes is included in the GH70 family, corresponding to enzymes that display no polymerase activity, but efficiently transfer the glucosyl residue from sucrose to linear alpha(1->6) dextrans through the specific formation of single alpha(1->2) or alpha(1->3) linked glucosyl units. They are named branching sucrases (BRSs)
-
evolution
Leuconostoc citreum NRRL B-1299
-
a distict subgroup of sucrose-active enzymes is included in the GH70 family, corresponding to enzymes that display no polymerase activity, but efficiently transfer the glucosyl residue from sucrose to linear alpha(1->6) dextrans through the specific formation of single alpha(1->2) or alpha(1->3) linked glucosyl units. They are named branching sucrases (BRSs)
-
physiological function
-
enzyme BrsB is specific for dextran branching via alpha-(1->3) linkage synthesis
physiological function
-
enzyme BrsB is specific for dextran branching via alpha-(1->3) linkage synthesis
-
additional information
-
branching induces a glass transition (Tg) depression (-10/-20°C) for amylopectin vs amylose and (-5/-10°C) for phytoglycogen vs amylopectin, which can be interpreted in terms of internal plasticization
additional information
-
branching induces a glass transition (Tg) depression (-10/-20°C) for amylopectin vs amylose and (-5/-10°C) for phytoglycogen vs amylopectin, which can be interpreted in terms of internal plasticization
-
additional information
Leuconostoc citreum NRRL B-1299
-
branching induces a glass transition (Tg) depression (-10/-20°C) for amylopectin vs amylose and (-5/-10°C) for phytoglycogen vs amylopectin, which can be interpreted in terms of internal plasticization
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 130000, isolated domain CD2, SDS-PAGE, x * 121000, isolated domain CD2, calculated from sequence
?
-
x * 130000, isolated domain CD2, SDS-PAGE, x * 121000, isolated domain CD2, calculated from sequence
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
construction of the GtfZ-CD2 mutant, a truncated variant comprising the catalytic domain CD2 (amino acids 121-2264) of the GtfZ protein of Lactobacillus kunkeei strain DSM 12361
additional information
-
construction of the GtfZ-CD2 mutant, a truncated variant comprising the catalytic domain CD2 (amino acids 121-2264) of the GtfZ protein of Lactobacillus kunkeei strain DSM 12361
-
additional information
-
expression of a truncated form deleted of the signal peptide and all APY motifs at the C-terminal end, for purification purpose
additional information
-
expression of a truncated form deleted of the signal peptide and all APY motifs at the C-terminal end, for purification purpose
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His-tagged enzyme mutant GtfZ-CD2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of domain CD2, amino acid residues 1212-2264 of the enzyme
-
gene gtfB, recombinant expression of C-terminally His-tagged enzyme mutant GtfZ-CD2 in Escherichia coli strain BL21(DE3)
-
recombinant expression of BRS-BDELTA1 enzyme in Escherichia coli strain BL21
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
-
using a suitable sucrose/dextran ratio, a comb-like dextran with 50% ofx02alpha-(1->3) branching can be synthesized
synthesis
-
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview
synthesis
-
using a suitable sucrose/dextran ratio, a comb-like dextran with 50% ofx02alpha-(1->3) branching can be synthesized
-
synthesis
-
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview
-
synthesis
Leuconostoc citreum NRRL B-1299
-
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Meng, X.; Gangoiti, J.; Wang, X.; Grijpstra, P.; van Leeuwen, S.; Pijning, T.; Dijkhuizen, L.
Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity
Appl. Microbiol. Biotechnol.
102
7935-7950
2018
Apilactobacillus kunkeei, Apilactobacillus kunkeei DSM 12361
-
brenda
Moulis, C.; Andre, I.; Remaud-Simeon, M.
GH13 amylosucrases and GH70 branching sucrases, atypical enzymes in their respective families
Cell. Mol. Life Sci.
73
2661-2679
2016
Leuconostoc citreum, Leuconostoc citreum NRRL B-742
brenda
Vuillemin, M.; Claverie, M.; Brison, Y.; Severac, E.; Bondy, P.; Morel, S.; Monsan, P.; Moulis, C.; Remaud-Simeon, M.
Characterization of the first alpha-(1->3) branching sucrases of the GH70 family
J. Biol. Chem.
291
7687-7702
2016
Leuconostoc citreum, Leuconostoc citreum NRRL B-742
brenda
Faucard, P.; Grimaud, F.; Lourdin, D.; Maigret, J.; Moulis, C.; Remaud-Simeon, M.; Putaux, J.; Potocki-Veronese, G.; Rolland-Sabate, A.
Macromolecular structure and film properties of enzymatically-engineered high molar mass dextrans
Carbohydr. Polym.
181
337-344
2018
Leuconostoc citreum, Leuconostoc citreum NRRL B-742
brenda
Te Poele, E.; Corwin, S.; Hamaker, B.R.; Lamothe, L.; Vafeiadi, C.; Dijkhuizen, L.
Development of slowly digestible starch derived alpha-glucans with 4,6-alpha-glucanotransferase and branching sucrase enzymes
J. Agric. Food Chem.
68
6664-6671
2020
Apilactobacillus kunkeei, Apilactobacillus kunkeei DSM 12361
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 2.4.1.362)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
Server: brenda6