Application | Comment | Organism |
---|---|---|
synthesis | using a suitable sucrose/dextran ratio, a comb-like dextran with 50% ofx02alpha-(1->3) branching can be synthesized | Leuconostoc citreum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leuconostoc citreum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of a truncated form deleted of the signal peptide and all APY motifs at the C-terminal end, for purification purpose | Leuconostoc citreum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
sucrose | no cosubstrate, pH 5.8, 30°C | Leuconostoc citreum | |
112 | - |
Dextran | pH 5.8, 30°C | Leuconostoc citreum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc citreum | - |
- |
- |
Leuconostoc citreum NRRL B-742 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
truncated protein variant | Leuconostoc citreum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | - |
Leuconostoc citreum | D-glucose + leucrose | - |
? | |
sucrose | - |
Leuconostoc citreum NRRL B-742 | D-glucose + leucrose | - |
? | |
sucrose + dextran | dextran of 70 kDa | Leuconostoc citreum | D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch | - |
? | |
sucrose + dextran | dextran of 70 kDa | Leuconostoc citreum NRRL B-742 | D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch | - |
? |
Synonyms | Comment | Organism |
---|---|---|
branching sucrase B | - |
Leuconostoc citreum |
BrsB | - |
Leuconostoc citreum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Leuconostoc citreum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
81.4 | - |
sucrose | no cosubstrate, pH 5.8, 30°C | Leuconostoc citreum | |
949 | - |
sucrose | cosubstrate dextran, pH 5.8, 30°C | Leuconostoc citreum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Leuconostoc citreum |