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Literature summary for 2.4.1.362 extracted from

  • Te Poele, E.; Corwin, S.; Hamaker, B.R.; Lamothe, L.; Vafeiadi, C.; Dijkhuizen, L.
    Development of slowly digestible starch derived alpha-glucans with 4,6-alpha-glucanotransferase and branching sucrase enzymes (2020), J. Agric. Food Chem., 68, 6664-6671 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.362

Cloned(Commentary)

Cloned (Comment) Organism
gene gtfB, recombinant expression of C-terminally His-tagged enzyme mutant GtfZ-CD2 in Escherichia coli strain BL21(DE3) Apilactobacillus kunkeei

Protein Variants

Protein Variants Comment Organism
additional information construction of the GtfZ-CD2 mutant, a truncated variant comprising the catalytic domain CD2 (amino acids 121-2264) of the GtfZ protein of Lactobacillus kunkeei strain DSM 12361 Apilactobacillus kunkeei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (1->6)-alpha-D-glucan Apilactobacillus kunkeei
-
 D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan Apilactobacillus kunkeei DSM 12361
-
 D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?

Organism

Organism UniProt Comment Textmining
Apilactobacillus kunkeei
-
-
-
Apilactobacillus kunkeei DSM 12361
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme mutant GtfZ-CD2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Apilactobacillus kunkeei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + sucrose dextran Mw is 70 kDa, transglucosylation activity Apilactobacillus kunkeei D-fructose + dextran containing a (1->3)-alpha-D-glucose branch
-
 ?
dextran + sucrose dextran Mw is 70 kDa, transglucosylation activity Apilactobacillus kunkeei DSM 12361 D-fructose + dextran containing a (1->3)-alpha-D-glucose branch
-
 ?
isomalto/malto-polysaccharide + sucrose alpha(1->3)-branched IMMP, IMMP Mw is 70 kDa, transglucosylation activity Apilactobacillus kunkeei D-fructose + isomalto/malto-polysaccharide containing a (1->3)-alpha-D-glucose branch
-
 ?
additional information enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages) Apilactobacillus kunkeei ?
-
-
additional information enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages) Apilactobacillus kunkeei DSM 12361 ?
-
-
sucrose + (1->6)-alpha-D-glucan
-
 Apilactobacillus kunkeei D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan transglucosylation activity Apilactobacillus kunkeei D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan
-
 Apilactobacillus kunkeei DSM 12361 D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan transglucosylation activity Apilactobacillus kunkeei DSM 12361 D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?
sucrose + H2O sucrose hydrolysis Apilactobacillus kunkeei D-fructose + D-glucose
-
 ?
sucrose + H2O sucrose hydrolysis Apilactobacillus kunkeei DSM 12361 D-fructose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
branching sucrase
-
 Apilactobacillus kunkeei
GtfZ
-
 Apilactobacillus kunkeei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Apilactobacillus kunkeei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 purified recombinant enzyme, 20 min, inactivation Apilactobacillus kunkeei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Apilactobacillus kunkeei