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Information on EC 2.4.1.147 - acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase and Organism(s) Homo sapiens and UniProt Accession Q6ZMB0

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EC Tree
IUBMB Comments
The product of the enzyme is known as core 3, one of the eight core structures of mucin-type O-glycans. O-Linked glycans are polysaccharides or oligosaccharides that are linked to a protein via the oxygen atom in the side chain of an L-serine or L-threonine residue.
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This record set is specific for:
Homo sapiens
UNIPROT: Q6ZMB0
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
hide
UDP-N-acetyl-alpha-D-glucosamine
+
O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
=
UDP
+
O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
+
O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
=
+
O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
Synonyms
uridine diphosphate, core 3 synthase, beta3gn-t6, b3gnt6, c3gnt, core3 synthase, core 3 beta1,3-n-acetylglucosaminyltransferase, beta3-n-acetylglucosaminyltransferase-6, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C3 beta3GnT6
-
core 3 beta3-N-acetylglucosaminyltransferase 6
-
core 3 synthase
-
UDPGlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
-
uridine diphosphate
-
[UDP]-GlcNAc:betaGal beta1,3-N-acetylglucosaminyltransferase
-
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-mucin beta(1-3)-
-
-
-
-
beta3-N-acetylglucosaminyltransferase-6
-
-
beta3Gn-T6
-
-
beta3GnT-6
-
-
core 3 beta3-GlcNAc-T
-
-
core 3 synthase
-
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core 3beta-GlcNAc-transferase
-
-
-
-
core3 O-glycan synthase
-
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core3 synthase
-
-
mucin core 3 beta3-GlcNAc-transferase
-
-
-
-
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
-
-
-
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uridine diphosphoacetylglucosamine-mucin beta(1-3)-acetylglucosaminyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase
The product of the enzyme is known as core 3, one of the eight core structures of mucin-type O-glycans. O-Linked glycans are polysaccharides or oligosaccharides that are linked to a protein via the oxygen atom in the side chain of an L-serine or L-threonine residue.
CAS REGISTRY NUMBER
COMMENTARY hide
87927-96-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-GlcNAc + 4-nitrophenyl-alpha-N-acetylgalactosamine
UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosamine
show the reaction diagram
-
-
-
?
UDP-GlcNAc + GlcNAc
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosamine
show the reaction diagram
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
UDP + O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-nitrophenyl N-acetyl-alpha-D-galactosaminide
UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminide
show the reaction diagram
recombinant protein, best substrate
-
-
?
UDP-N-acetyl-D-glucosamine + asialofetuin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-asialofetuin
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-alpha-D-galactosaminyl-4-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-alpha-D-galactosaminyl-4-nitrophenyl
show the reaction diagram
recombinant protein, 2.4% activity compared to N-acetyl-beta-D-galactosaminyl-alpha-4-nitrophenyl
-
-
?
UDP-N-acetyl-D-glucosamine + bovine submaxillary gland mucin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-mucin of bovine submaxillary gland
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-4-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-4-nitrophenyl
show the reaction diagram
recombinant protein, 15% activity compared to N-acetyl-beta-D-galactosaminyl-alpha-4-nitrophenyl
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-MUC1 mucin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-MUC1 mucin
show the reaction diagram
-
N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosamine is bound to the glycoprotein via serine or threonine residues, resulting in a core 3 structure
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-Ser/Thr
UDP + N-acetyl-beta-D-glucosaminyl-(1->)3-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 2-azido-2-deoxy-alpha-D-galactosyl-benzyl
UDP + 3-O-(N-acetyl-beta-D-glucosaminyl)-2-azido-2-deoxy-alpha-D-galactosyl-benzyl
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-nitrophenyl N-acetyl-alpha-D-galactosaminide
UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminide
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-galactosaminyl-4-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-alpha-D-galactosaminyl-4-nitrophenyl
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-(CH2)2NHCO(CH2)3CO2CH3
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-(CH2)2NHCO(CH2)3CO2CH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + ovine submaxillary gland mucin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-mucin of ovine submaxillary gland
show the reaction diagram
-
acceptor substrate is desialylated, degalactosylated, defucosylated
-
-
?
additional information
?
-
-
expression of the enzyme is closely regulated during differentiation and dedifferentiation. Rge expression of the enzyme dramatically decreased in cancerous tissues, and the changes to the core structures of O-glycans give rise to phenotypic changes of cancer cells, such as mobility and metastatic activity
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
UDP + O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-Ser/Thr
UDP + N-acetyl-beta-D-glucosaminyl-(1->)3-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
expression of the enzyme is closely regulated during differentiation and dedifferentiation. Rge expression of the enzyme dramatically decreased in cancerous tissues, and the changes to the core structures of O-glycans give rise to phenotypic changes of cancer cells, such as mobility and metastatic activity
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
dependent on, physiological Mn2+ concentration in Saccharomyces cerevisiae is insufficient to facilitate optimal synthesis of core 3. Mn2+ supplementation led to upregulation of reaction of glycosylation in the Golgi, resulting in increases of core 3 production. Although the supply of Mn2+ in the Golgi apparatus is essential for glycosylation, excess Mn2+ levels are toxic to yeast
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-iodoacetamido-galactosamine-alpha-benzyl
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colon homogenates, 50% inhibition at 2 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00009
-
colon mucosa
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
assay at
6.5 - 7
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low amount
Manually annotated by BRENDA team
-
expression of the enzyme is markedly down-regulated in gastric and colorectal carcinomas
Manually annotated by BRENDA team
-
in normal stomach and colon the enzyme is strongly expressed in the Golgi region of the epithelium
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
in normal stomach and colon the enzyme is strongly expressed in the Golgi region of the epithelium
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
O-glycan biosynthesis pathways and proposed O-glycan structures of selected glycosyltransferases, overview. GalNAc alpha1-Ser/Thr is converted by C3 beta3GnT6 to core 3 O-glycans. Regulation of O-glycan biosynthesis, overview
physiological function
in humans, the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-O-Ser/Thr) has been found in mucins from specific tissues such as the stomach, small intestine, and colon, and the human gene (B3GNT6) encoding the core 3 synthase (uridine diphosphate [UDP]-GlcNAc:betaGal beta1,3-N-acetylglucosaminyltransferase, beta3Gn-T6) is involved in core 3 synthesis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
B3GN6_HUMAN
384
1
42748
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
x * 43000, amino acid sequence determination
46600
x * 46600, recombinant FLAG-tagged fusion protein, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
recombinant FLAG-tagged fusion protein is glycosylated in insect cells
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is extremly unstable in solubilized form
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant from Sf21 insect cells as FLAG-tagged fusion protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Spodoptera frugiperda Sf21 cells via baculovirus infection as soluble FLAG-tagged fusion protein
gene B3GNT6, recombinant expression in CHO-K1 cells. Glycosylation-related genes in CHO-K1 cells are present, but strictly suppressed and regulated. CHO cells are transiently co-transfected with plasmids encoding extended C1 beta3GnT3, C2 beta6GnT1, or C3 beta3GnT6 with or without ST6Gal I or CHST4, respectively, and resulting O-glycan and core chain spectrum, overview
gene B3GNT6, recombinant expression in Saccharomyces cerevisiae, the human beta3Gn-T6 is produced as a protein fused with the transmembrane domains of Saccharomyces cerevisiae glycosyltransferases (ScMnn9p, ScMnn2p, ScMnn1p, or ScGnt1p), thereby localizing beta3Gn-T6 to the Golgi, optimization of beta3Gn-T6 expression
expressed in PC3 and LNCaP prostate cancer cell lines
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
the enzyme would be a useful marker for distinguishing between benign adenomas and premalignant lesions
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schachter, H.; Brockhausen, I.; Hull, E.
High-performance liquid chromatography assays for N-acetylglucosaminyltransferases involved in N- and O-glycan synthesis
Methods Enzymol.
179
351-397
1989
Homo sapiens, no activity in Canis familiaris, Ovis aries, Platyrrhini, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Brockhausen, I.; Matta, K.L.; Orr, J.; Schachter, H.
Mucin synthesis. UDP-GlcNAc:GalNAc-R beta 3-N-acetylglucosaminyltransferase and UDP-GlcNAc:GlcNAc beta 1-3GalNAc-R (GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase from pig and rat colon mucosa
Biochemistry
24
1866-1874
1985
Ovis aries, Homo sapiens, Platyrrhini, no activity in Canis familiaris, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Vavasseur, F.; Yang, J.M.; Dole, K.; Paulsen, H.; Brockhausen, I.
Synthesis of O-glycan core 3: characterization of UDP-GlcNAc:GalNAc-R beta3-N-acetyl-glucosaminyltransferase activity from colonic mucosal tissues and lack of the activity in human cancer cell lines
Glycobiology
5
351-357
1995
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Iwai, T.; Inaba, N.; Naundorf, A.; Zhang, Y.; Gotoh, M.; Iwasaki, H.; Kudo, T.; Togayachi, A.; Ishizuka, Y.; Nakanishi, H.; Narimatsu, H.
Molecular cloning and characterization of a novel UDP-GlcNAc:GalNAc-peptide beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T6), an enzyme synthesizing the core 3 structure of O-glycans
J. Biol. Chem.
277
12802-12809
2002
Homo sapiens (Q6ZMB0), Homo sapiens
Manually annotated by BRENDA team
Iwai, T.; Kudo, T.; Kawamoto, R.; Kubota, T.; Togayachi, A.; Hiruma, T.; Okada, T.; Kawamoto, T.; Morozumi, K.; Narimatsu, H.
Core 3 synthase is down-regulated in colon carcinoma and profoundly suppresses the metastatic potential of carcinoma cells
Proc. Natl. Acad. Sci. USA
102
4572-4577
2005
Homo sapiens
Manually annotated by BRENDA team
Lee, S.; Hatakeyama, S.; Yu, S.; Bao, X.; Ohyama, C.; Khoo, K.; Fukuda, M.; Fukuda, M.
Core3 O-glycan synthase suppresses tumor formation and metastasis of prostate carcinoma PC3 and LNCaP cells through down-regulation of alpha2beta1 integrin complex
J. Biol. Chem.
284
17157-17169
2009
Homo sapiens
Manually annotated by BRENDA team
Liu, J.; Jin, C.; Cherian, R.M.; Karlsson, N.G.; Holgersson, J.
O-glycan repertoires on a mucin-type reporter protein expressed in CHO cell pools transiently transfected with O-glycan core enzyme cDNAs
J. Biotechnol.
199
77-89
2015
Homo sapiens (Q6ZMB0)
Manually annotated by BRENDA team
Saito, F.; Sakamoto, I.; Kanatani, A.; Chiba, Y.
Manganese ion concentration affects production of human core 3 O-glycan in Saccharomyces cerevisiae
Biochim. Biophys. Acta
1860
1809-1820
2016
Homo sapiens (Q6ZMB0), Homo sapiens
Manually annotated by BRENDA team