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Literature summary for 2.4.1.147 extracted from
- Mucin synthesis. UDP-GlcNAc:GalNAc-R beta 3-N-acetylglucosaminyltransferase and UDP-GlcNAc:GlcNAc beta 1-3GalNAc-R (GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase from pig and rat colon mucosa (1985), Biochemistry, 24, 1866-1874.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulation, 0.1% v/v, inhibits at concentrations above 0.5% v/v | Rattus norvegicus |  |
| Triton X-100 | stimulation, 0.1% v/v, inhibits at concentrations above 0.5% v/v | Sus scrofa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | in the presence of Mn2+ | Sus scrofa | |
| additional information | no inhibition by 2-mercaptoethanol or N-acetyl-D-glucosamine | Sus scrofa | |
| Triton X-100 | - |
Rattus norvegicus | |
| Triton X-100 | above 0.5% v/v | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
N-acetyl-D-galactosaminyl-alpha-benzyl | - |
Sus scrofa | |
| 3.2 | - |
N-acetyl-alpha-D-galactosaminyl-ovine submaxillary mucin | - |
Ovis aries | |
| 5 | - |
N-acetyl-D-galactosaminyl-alpha-phenyl | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Sus scrofa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | absolute requirement, 10-20 mM, cannot be replaced by Co2+, Ca2+, Mg2+ or Zn2+ | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| no activity in Canis familiaris | - |
submaxillary glands | - |
| Ovis aries | - |
- |
- |
| Platyrrhini | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colonic mucosa | - |
Homo sapiens | - |
| colonic mucosa | - |
Rattus norvegicus | - |
| colonic mucosa | - |
Sus scrofa | - |
| colonic mucosa | - |
Platyrrhini | - |
| additional information | - |
Homo sapiens | - |
| additional information | absent from submaxillary glands | Rattus norvegicus | - |
| additional information | absent from submaxillary glands | Sus scrofa | - |
| stomach | low activity | Rattus norvegicus | - |
| stomach | low activity | Sus scrofa | - |
| stomach | low activity | Ovis aries | - |
| stomach | low activity | Platyrrhini | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00009 | - |
colon mucosa | Homo sapiens |
| 0.00033 | - |
colon mucosa | Rattus norvegicus |
| 0.00034 | - |
colon mucosa | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-ovine submaxillary mucin | - |
Ovis aries | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-ovine submaxillary mucin | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl | - |
Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R | - |
Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R | no acceptor substrate: native ovine submaxillary mucin, containing sialyl-2,6-N-acetyl-D-galactosaminyl-groups | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-galactosyl-ovine submaxillary gland mucin | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-D-galactosyl-mucin of ovine submaxillary gland | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Sus scrofa |
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