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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Oryza sativa and UniProt Accession Q43009

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase
IUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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Select one or more organisms in this record:
This record set is specific for:
Oryza sativa
UNIPROT: Q43009
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The taxonomic range for the selected organisms is: Oryza sativa
Synonyms
CaSUS1, CaSUS2, CsSUS3, glucosyltransferase, uridine diphosphoglucose-fructose, LjSUS3, Msus1, MtSucS1, mtSUS, PsnSuSy2, RSuS3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucosyltransferase, uridine diphosphoglucose-fructose
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RSuS3
290363
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sucrose synthetase
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sucrose-UDP glucosyltransferase
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sucrose-uridine diphosphate glucosyltransferase
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SuSy
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UDP-glucose-fructose glucosyltransferase
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UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
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uridine diphosphoglucose-fructose glucosyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
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-
-
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r
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
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-
-
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r
IDP-glucose + D-fructose
IDP + sucrose
show the reaction diagram
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-
-
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?
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
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-
-
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r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required in sucrose synthesis reaction
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
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high concentrations
Ca2+
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sucrose cleavage
Cu2+
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no activity at 1 mM
Fe2+
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no activity at 1 mM
fructose
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high concentration, sucrose synthesis
Mg2+
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sucrose cleavage
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
abscisic acid
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as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
Ca2+
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only for sucrose synthesis
Mg2+
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only for sucrose synthesis
sucrose
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exogenous, as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.3 - 3.8
ADP
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0.44 - 0.9
CDP
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2.08 - 40
fructose
52 - 290
sucrose
0.65
TDP
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0.41
UDP
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1.92 - 6
UDPglucose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.33
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SUS activity in rice grain untreated during grain filling
0.45
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SUS activity in rice grain treated with abscisic acid during grain filling
0.53
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SUS activity in rice grain treated with sducrose and abscisic acid during grain filling
0.66
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SUS activity in rice grain treated with sucrose during grain filling
0.89
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3.54
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
sucrose cleavage
6
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sucrose cleavage
6.5 - 7
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in 0.2 M HEPES-NaOH, varies with buffer
7 - 9.5
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broad, sucrose synthesis
additional information
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pI: 6.16
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
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about 80% of maximal activity at pH 5.5 and about half-maximal activity at pH 9.0, sucrose cleavage
5.6 - 10
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about half-maximal activity at pH 5.6 and about 90% of maximal activity at pH 10.0, sucrose synthesis
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
50 - 60
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in 0.2 M Hepes-NaOH, varies with buffer
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to family 4 of the glycosyltransferases (GT4) and contains an E-X7-E motif that is conserved in members of GT4 and two other GT families, sequence comparisons, overview
metabolism
key enzyme in sucrose metabolism in higher plants
physiological function
the enzyme activity is finely tuned through regulation of their gene expression at different levels and modulation of enzymatic activities by metabolites
metabolism
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sucrose synthase is a key enzyme regulating the process of rice grain filling meaning conversion of sucrose into starch
additional information
enzyme homology modeling and substrate docking using the crystal structure of Nitrosomonas europaea NeSuS1, PDB ID 4RBN, and the crystal structure of Arabidopsis AtSuS1, PDB ID 3S28, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
336000 - 375000
recombinant wild-type and mutant enzymes, gel filtration
92000
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4 * 92000, SDS-PAGE
100000
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4 * 100000, SDS-PAGE
362000
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gel filtration
410000
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SDS-PAGE
440000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E678D
site-directed mutagenesis, almost inactive mutant
E678Q
site-directed mutagenesis, almost inactive mutant, the mutant shows impaired D-fructose binding
E686D
site-directed mutagenesis, the mutant enzyme retains 34.9% of sucrose cleavage and 37.9% of sucrose synthesis activity, respectively
E686Q
site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding
F680S
site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding
F680Y
site-directed mutagenesis, the mutant enzyme retains 61.0% of sucrose cleavage and all of sucrose synthesis activity, respectively
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 20% loss of activity within 2 weeks, t1/2: 1 month
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
recombinant wild-type and mutant enzyes from Pichia pastris by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
3 isozymes, heterotetramers of ss1, SS2 subunits
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no isozymes detected by isoelectric focusing
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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
gene SUS3, expression analysis in various rice tissues using real-time quantitative RT-PCR
sus3, recombinant expression of wild-type and mutant enzymes in Pichia pastoris
DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene SUS1, expression analysis in various rice tissues using real-time quantitative RT-PCR
gene SUS2, expression analysis in various rice tissues using real-time quantitative RT-PCR
gene SUS4, expression analysis in various rice tissues using real-time quantitative RT-PCR
gene SUS5, expression analysis in various rice tissues using real-time quantitative RT-PCR
gene SUS6, expression analysis in various rice tissues using real-time quantitative RT-PCR
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
abscisic acid, exogenous sucrose, as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling, determination of protein, overview
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Elling, L.; Kula, M.R.
Purification of sucrose synthase from rice and its protein-chemical characterization
J. Biotechnol.
29
277-286
1993
Oryza sativa
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Manually annotated by BRENDA team
Nomura, T.; Akazawa, T.
Enzymic mechanism of starch stynthesis in ripening rice grains. VII. Purification and enzymic properties of sucrose synthetase
Arch. Biochem. Biophys.
156
644-652
1973
Oryza sativa
Manually annotated by BRENDA team
Murata, T.
Sucrose synthetase of rice grains and potato tubers
Agric. Biol. Chem.
36
1815-1818
1972
Oryza sativa, Solanum tuberosum
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Manually annotated by BRENDA team
Elling, L.; Kula, M.R.
Characterization of sucrose synthase from rice grains for the enzymic synthesis of UDP and TDP glucose
Enzyme Microb. Technol.
17
929-934
1995
Oryza sativa
-
Manually annotated by BRENDA team
Huang, D.Y.; Wang, A.Y.
Purification and characterization of sucrose synthase isozymes from etiolated rice seedlings
Biochem. Mol. Biol. Int.
46
107-113
1998
Oryza sativa
Manually annotated by BRENDA team
Yen, S.F.; Su, J.C.; Sung, H.Y.
Purification and characterization of rice sucrose synthase isozymes
Biochem. Mol. Biol. Int.
34
613-620
1994
Oryza sativa
Manually annotated by BRENDA team
Abid, G.; Silue, S.; Muhovski, Y.; Jacquemin, J.M.; Toussaint, A.; Baudoin, J.P.
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development
Gene
439
1-10
2009
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Citrus unshiu (Q9SLY2), Citrus x paradisi, Coffea arabica (Q0E7D4), Daucus carota (O49845), Eucalyptus grandis (Q00P15), Eucalyptus grandis (Q00P16), Glycine max, Glycine max (P13708), Gossypium hirsutum, Gossypium hirsutum (Q9XGB7), Oryza sativa (P31924), Pisum sativum (O24301), Pisum sativum (O81610), Pisum sativum (Q9AVR8), Pisum sativum (Q9T0M9), Solanum lycopersicum (P49037), Solanum tuberosum (Q84T18), Vicia faba (P31926), Zea mays
Manually annotated by BRENDA team
Tang, T.; Xie, H.; Wang, Y.; Lue, B.; Liang, J.
The effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.)
J. Exp. Bot.
60
2641-2652
2009
Oryza sativa
Manually annotated by BRENDA team
Hirose, T.; Scofield, G.; Terao, T.
An expression analysis profile for the entire sucrose synthase gene family in rice
Plant Sci.
174
534-543
2008
Oryza sativa (H6TFZ4), Oryza sativa, Oryza sativa (P30298), Oryza sativa (P31924), Oryza sativa (Q10LP5), Oryza sativa (Q43009), Oryza sativa (Q6K973)
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Manually annotated by BRENDA team
Huang, Y.C.; Hsiang, E.C.; Yang, C.C.; Wang, A.Y.
New insight into the catalytic properties of rice sucrose synthase
Plant Mol. Biol.
90
127-135
2016
Oryza sativa, Oryza sativa (Q43009)
Manually annotated by BRENDA team
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