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EC Number	Protein Variants	Commentary	Reference
2.4.1.13	A642N	site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme	-, 735556
2.4.1.13	E678D	site-directed mutagenesis, almost inactive mutant	737015
2.4.1.13	E678Q	site-directed mutagenesis, almost inactive mutant, the mutant shows impaired D-fructose binding	737015
2.4.1.13	E686D	site-directed mutagenesis, the mutant enzyme retains 34.9% of sucrose cleavage and 37.9% of sucrose synthesis activity, respectively	737015
2.4.1.13	E686Q	site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding	737015
2.4.1.13	F680S	site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding	737015
2.4.1.13	F680Y	site-directed mutagenesis, the mutant enzyme retains 61.0% of sucrose cleavage and all of sucrose synthesis activity, respectively	737015
2.4.1.13	K639R	the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme	-, 758272
2.4.1.13	L636Q/A642N	site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme	-, 735556
2.4.1.13	L636Q/A642N	the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme	-, 758272

Results 1 - 10 of 42

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Release 2023.1 (January 2023)