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Literature summary for 2.4.1.13 extracted from
- Enzymic mechanism of starch stynthesis in ripening rice grains. VII. Purification and enzymic properties of sucrose synthetase (1973), Arch. Biochem. Biophys., 156, 644-652.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | high concentrations | Oryza sativa |  |
| fructose | high concentration, sucrose synthesis | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | 0.9 | CDP | - |
Oryza sativa | |
| 3.3 | 3.8 | ADP | - |
Oryza sativa | |
| 5.3 | 6 | UDPglucose | - |
Oryza sativa | |
| 40 | - |
fructose | cosubstrate ADPglucose | Oryza sativa | |
| 52 | 290 | sucrose | cosubstrate UDP | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
4 * 100000, SDS-PAGE | Oryza sativa |
| 410000 | - |
SDS-PAGE | Oryza sativa |
| 440000 | - |
gel filtration | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | ripening | Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + D-fructose | - |
Oryza sativa | ADP + sucrose | - |
r | |
| CDP-glucose + D-fructose | - |
Oryza sativa | CDP + sucrose | - |
? | |
| UDP + sucrose | also substrate: ADP | Oryza sativa | UDP-glucose + D-fructose | - |
? | |
| UDPglucose + D-fructose | - |
Oryza sativa | UDP + sucrose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 100000, SDS-PAGE | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
sucrose cleavage | Oryza sativa |
| 7 | 9.5 | broad, sucrose synthesis | Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | about 80% of maximal activity at pH 5.5 and about half-maximal activity at pH 9.0, sucrose cleavage | Oryza sativa |
| 5.6 | 10 | about half-maximal activity at pH 5.6 and about 90% of maximal activity at pH 10.0, sucrose synthesis | Oryza sativa |
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Release 2023.1 (January 2023)
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