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Information on EC 2.3.1.47 - 8-amino-7-oxononanoate synthase and Organism(s) Bacillus subtilis and UniProt Accession P53556

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IUBMB Comments
A pyridoxal-phosphate protein. The enzyme catalyses the decarboxylative condensation of L-alanine and pimeloyl-[acyl-carrier protein], a key step in the pathway for biotin biosynthesis. Pimeloyl-CoA can be used with lower efficiency .
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This record set is specific for:
Bacillus subtilis
UNIPROT: P53556
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
dapa at, 8-amino-7-oxononanoate synthase, 7,8-diaminopelargonic acid aminotransferase, 8-amino-7-oxopelargonate synthase, kapa synthase, kapas, biof protein, 7-keto-8-aminopelargonic acid synthase, 7-keto-8-aminopelargonic acid synthetase, atbiof, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7-keto-8-aminopelargonate synthase
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7-keto-8-aminopelargonate synthetase
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7-keto-8-aminopelargonic acid synthetase
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7-keto-8-aminopelargonic synthetase
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7-oxo-8-aminononanoate synthase
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8-amino-7-oxopelargonate synthase
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KAPA synthetase
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synthase, 7-oxo-8-aminononanoate
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synthetase, 7-keto-8-aminopelargonate
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
6-carboxyhexanoyl-[acyl-carrier protein]:L-alanine C-carboxyhexanoyltransferase (decarboxylating)
A pyridoxal-phosphate protein. The enzyme catalyses the decarboxylative condensation of L-alanine and pimeloyl-[acyl-carrier protein], a key step in the pathway for biotin biosynthesis. Pimeloyl-CoA can be used with lower efficiency [5].
CAS REGISTRY NUMBER
COMMENTARY hide
9075-61-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pimeloyl-CoA + L-alanine
8-amino-7-oxononanoate + CO2 + CoA
show the reaction diagram
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-
?
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-carboxyhexanoyl-CoA + L-alanine
8-amino-7-oxononanoate + CoA + CO2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
dependent on
pyridoxal 5'-phosphate
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D/L-histidine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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crude cell-extracts from various organism
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Izumi, Y.; Sato, K.; Tani, Y.; Ogata, K.
Distribution of 7-keto-8-aminopelargonic acid synthetase in bacteria and the control mechanism of the enzyme activity
Agric. Biol. Chem.
37
1335-1340
1973
Alcaligenes faecalis, Bacillus subtilis, Citrobacter freundii, Enterobacter cloacae, Erwinia aroidea, Escherichia coli, Klebsiella aerogenes, Kocuria rosea, Lysinibacillus sphaericus, Pseudomonas fluorescens
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Manually annotated by BRENDA team
Manandhar, M.; Cronan, J.E.
A Canonical biotin synthesis enzyme, 8-amino-7-oxononanoate synthase (BioF), utilizes different acyl chain donors in Bacillus subtilis and Escherichia coli
Appl. Environ. Microbiol.
84
e02084-17
2018
Bacillus subtilis (P53556), Bacillus subtilis, Bacillus subtilis 168 (P53556), Escherichia coli (P12998), Escherichia coli
Manually annotated by BRENDA team