Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.47 extracted from

  • Manandhar, M.; Cronan, J.E.
    A Canonical biotin synthesis enzyme, 8-amino-7-oxononanoate synthase (BioF), utilizes different acyl chain donors in Bacillus subtilis and Escherichia coli (2018), Appl. Environ. Microbiol., 84, e02084-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P53556
-
-
Bacillus subtilis 168 P53556
-
-
Escherichia coli P12998
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is unable to utilize pimeloyl-[acyl carrier protein] Bacillus subtilis ?
-
-
additional information the enzyme is unable to utilize pimeloyl-[acyl carrier protein] Bacillus subtilis 168 ?
-
-
pimeloyl-CoA + L-alanine
-
 Escherichia coli 8-amino-7-oxononanoate + CO2 + CoA
-
 ?
pimeloyl-CoA + L-alanine
-
 Bacillus subtilis 8-amino-7-oxononanoate + CO2 + CoA
-
 ?
pimeloyl-CoA + L-alanine
-
 Bacillus subtilis 168 8-amino-7-oxononanoate + CO2 + CoA
-
 ?
pimeloyl-[acyl carrier protein] + L-alanine
-
 Escherichia coli 8-amino-7-oxononanoate + CO2 + [acyl carrier protein]
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Escherichia coli
? x * 37000, SDS-PAGE Bacillus subtilis
? x * 42500, calculated from amino acid sequence Bacillus subtilis
? x * 43100, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
7-keto-8-aminopelargonate synthase
-
 Escherichia coli
7-keto-8-aminopelargonate synthase
-
 Bacillus subtilis
AON synthase
-
 Escherichia coli
AON synthase
-
 Bacillus subtilis
BioF
-
 Escherichia coli
BioF
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Escherichia coli
pyridoxal 5'-phosphate dependent on Bacillus subtilis