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Information on EC 2.1.1.181 - 23S rRNA (adenine1618-N6)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P75782

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IUBMB Comments
The recombinant YbiN protein is able to methylate partially deproteinized 50 S ribosomal subunit, but neither the completely assembled 50 S subunits nor completely deproteinized 23 S rRNA .
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This record set is specific for:
Escherichia coli
UNIPROT: P75782
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
ybin protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
m6A1618 methyltransferase
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rRNA large subunit methyltransferase F
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YbiN protein
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (adenine1618-N6)-methyltransferase
The recombinant YbiN protein is able to methylate partially deproteinized 50 S ribosomal subunit, but neither the completely assembled 50 S subunits nor completely deproteinized 23 S rRNA [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
show the reaction diagram
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
knockout of the ybiN gene leads to loss of modification at adenine1618. The modification is restored if ybiN knock-out strain has been co-transformed with a plasmid expressing the ybiN gene. Both lack of the ybiN gene and it's overexpression leads to growth retardation and loss of cell fitness comparative to the parental strain
physiological function
A1618 modification could be necessary for the exit tunnel interaction with some unknown regulatory peptides
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
x * 34000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 34000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sergiev, P.; Serebryakova, M.; Bogdanov, A.; Dontsova, O.
The ybiN gene of Escherichia coli encodes adenine-N6 methyltransferase specific for Modification of A1618 of 23 S ribosomal RNA, a methylated residue located close to the ribosomal exit tunnel
J. Mol. Biol.
375
291-300
2008
Escherichia coli (P75782), Escherichia coli
Manually annotated by BRENDA team