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Literature summary for 2.1.1.181 extracted from

  • Sergiev, P.; Serebryakova, M.; Bogdanov, A.; Dontsova, O.
    The ybiN gene of Escherichia coli encodes adenine-N6 methyltransferase specific for Modification of A1618 of 23 S ribosomal RNA, a methylated residue located close to the ribosomal exit tunnel (2008), J. Mol. Biol., 375, 291-300.
    View publication on PubMed
Search for more literature for 2.1.1.181

Cloned(Commentary)

Cloned (Comment) Organism
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine1618 in 23S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P75782
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + adenine1618 in 23S rRNA the recombinant YbiN protein is able to methylate partially deproteinized 50 S ribosomal subunit, but neither the completely assembled 50 S subunit nor completely deproteinized 23 S rRNA Escherichia coli S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
m6A1618 methyltransferase
-
 Escherichia coli
rlmF
-
 Escherichia coli
rRNA large subunit methyltransferase F
-
 Escherichia coli
ybiN
-
 Escherichia coli
YbiN protein
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction knockout of the ybiN gene leads to loss of modification at adenine1618. The modification is restored if ybiN knock-out strain has been co-transformed with a plasmid expressing the ybiN gene. Both lack of the ybiN gene and it's overexpression leads to growth retardation and loss of cell fitness comparative to the parental strain Escherichia coli
physiological function A1618 modification could be necessary for the exit tunnel interaction with some unknown regulatory peptides Escherichia coli