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Information on EC 2.1.1.137 - arsenite methyltransferase

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.137 arsenite methyltransferase
IUBMB Comments
An enzyme responsible for synthesis of trivalent methylarsenical antibiotics in microbes or detoxification of inorganic arsenous acid in animals. The in vivo substrate is arsenic triglutathione or similar thiol (depending on the organism) , from which the arsenic is transferred to the enzyme forming bonds with the thiol groups of three cysteine residues via a disulfide bond cascade pathway [7, 8]. Most of the substrates undergo two methylations and are converted to dimethylarsinous acid . However, a small fraction are released earlier as methylarsonous acid, and a smaller amount proceeds via a third methylation, resulting in the volatile product trimethylarsane. Methylation involves temporary oxidation to arsenic(V) valency, followed by reduction back to arsenic(III) valency using electrons provided by thioredoxin or a similar reduction system. The arsenic(III) products are quickly oxidized in the presence of oxygen to the corresponding arsenic(V) species.
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This record set is specific for:
UNIPROT: C0JV69
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
as3mt, arsenic (+3 oxidation state) methyltransferase, n6amt1, has3mt, arsenite methyltransferase, cyt19, as(iii) s-adenosylmethionine methyltransferase, arsenic (iii) methyltransferase, arsenite s-adenosylmethionine methyltransferase, arsenic (+3) methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ArsM
Cyanidioschyzon sp.
-
ArsM7B
Cyanidioschyzon sp.
-
As(III) S-adenosylmethionine methyltransferase
Cyanidioschyzon sp.
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ArsM
-
-
-
-
AS3MT
-
-
-
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methylarsonite methyltransferase
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-
-
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S-adenosyl-L-methionine:arsenic(III) methyltransferase
-
-
-
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S-adenosyl-L-methionine:methylarsonite As-methyltransferase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of methyl group
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:arsenous acid As-methyltransferase
An enzyme responsible for synthesis of trivalent methylarsenical antibiotics in microbes [11] or detoxification of inorganic arsenous acid in animals. The in vivo substrate is arsenic triglutathione or similar thiol (depending on the organism) [6], from which the arsenic is transferred to the enzyme forming bonds with the thiol groups of three cysteine residues [10] via a disulfide bond cascade pathway [7, 8]. Most of the substrates undergo two methylations and are converted to dimethylarsinous acid [9]. However, a small fraction are released earlier as methylarsonous acid, and a smaller amount proceeds via a third methylation, resulting in the volatile product trimethylarsane. Methylation involves temporary oxidation to arsenic(V) valency, followed by reduction back to arsenic(III) valency using electrons provided by thioredoxin or a similar reduction system. The arsenic(III) products are quickly oxidized in the presence of oxygen to the corresponding arsenic(V) species.
CAS REGISTRY NUMBER
COMMENTARY hide
167140-41-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + arsenite
S-adenosyl-L-homocysteine + methylarsonate
show the reaction diagram
Cyanidioschyzon sp.
-
-
-
?
S-adenosyl-L-methionine + methylarsonite
S-adenosyl-L-homocysteine + dimethylarsinate
show the reaction diagram
Cyanidioschyzon sp.
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + arsenite
S-adenosyl-L-homocysteine + methylarsonate
show the reaction diagram
Cyanidioschyzon sp.
-
-
-
?
S-adenosyl-L-methionine + methylarsonite
S-adenosyl-L-homocysteine + dimethylarsinate
show the reaction diagram
Cyanidioschyzon sp.
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Cyanidioschyzon sp.
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UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C0JV69_9RHOD
400
0
43727
TrEMBL
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44980
Cyanidioschyzon sp.
x * 44980, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
Cyanidioschyzon sp.
x * 44980, calculated from amino acid sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3000, 0.1 M Tris-HCl, pH 7.0, containing 0.2 M calcium acetate
Cyanidioschyzon sp.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
-
Cyanidioschyzon sp.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
Cyanidioschyzon sp.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ajees, A.A.; Marapakala, K.; Packianathan, C.; Sankaran, B.; Rosen, B.P.
Structure of an As(III) S-adenosylmethionine methyltransferase: insights into the mechanism of arsenic biotransformation
Biochemistry
51
5476-5485
2012
Cyanidioschyzon sp. (C0JV69)
Manually annotated by BRENDA team