Information on EC 1.5.5.3 - hydroxyproline dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.5.5.3
-
RECOMMENDED NAME
GeneOntology No.
hydroxyproline dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
trans-4-hydroxy-L-proline + a quinone = (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + a quinol
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
trans-4-hydroxy-L-proline degradation I
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Arginine and proline metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
trans-4-hydroxy-L-proline:quinone oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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enzyme knockdown alters glycolate and oxalate levels
physiological function
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the enzyme plays a role in growth regulation, reactive oxygen species generation, and activation of the apoptotic cascade
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-proline + coenzyme Q1
?
show the reaction diagram
-
-
-
?
trans-4-hydroxy-L-proline + a quinone
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + a quinol
show the reaction diagram
trans-4-hydroxy-L-proline + coenzyme Q1
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
show the reaction diagram
-
-
-
?
trans-4-hydroxy-L-proline + coenzyme Q10
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
show the reaction diagram
preferred substrate
-
-
?
trans-4-hydroxy-L-proline + cytochrome c
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
trans-4-hydroxy-L-proline + duroquinone
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + duroquinol
show the reaction diagram
-
-
-
?
trans-4-hydroxy-L-proline + menadione
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menadiol
show the reaction diagram
-
-
-
?
trans-4-hydroxy-L-proline + menaquinone
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menaquinol
show the reaction diagram
-
-
-
?
trans-4-hydroxy-L-proline + O2
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trans-4-hydroxy-L-proline + a quinone
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + a quinol
show the reaction diagram
trans-4-hydroxy-L-proline + coenzyme Q10
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
show the reaction diagram
Q9UF12
preferred substrate
-
-
?
trans-4-hydroxy-L-proline + O2
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ?
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,3R)-3-hydroxy-cyclopentane-carboxylate
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5-hydroxy-1H-pyrazole-3-carboxylate
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menadione
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Tetrahydrofuroic acid
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additional information
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(1S,3R)-3-hydroxy-cyclopentane carboxylic acid, trans-4-hydroxy-D-proline, cis-4-hydroxy-L-proline, and cis-4-hydroxy-D-proline do not cause inhibition even up to 5 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.124
coenzyme Q1
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at pH 7.5 and 25°C
0.143
duroquinone
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at pH 7.5 and 25°C
0.025
menaquinone
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at pH 7.5 and 25°C
200
trans-4-hydroxy-L-proline
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at pH 7.5 and 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
coenzyme Q1
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at pH 7.5 and 25°C
0.27
duroquinone
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at pH 7.5 and 25°C
0.28
menaquinone
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at pH 7.5 and 25°C
0.19
trans-4-hydroxy-L-proline
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at pH 7.5 and 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
coenzyme Q1
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at pH 7.5 and 25°C
1.9
duroquinone
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at pH 7.5 and 25°C
75
L-proline
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at pH 7.5 and 25°C
11
menaquinone
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at pH 7.5 and 25°C
0.93
trans-4-hydroxy-L-proline
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at pH 7.5 and 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.306
menadione
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at pH 7.5 and 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1
(1R,3R)-3-hydroxy-cyclopentane-carboxylate
Homo sapiens;
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at pH 7.5 and 25°C
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2.9
5-hydroxy-1H-pyrazole-3-carboxylate
Homo sapiens;
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at pH 7.5 and 25°C
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1.5
Tetrahydrofuroic acid
Homo sapiens;
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at pH 7.5 and 25°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose column chromatography and HisPur cobalt resin column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41(DE3) cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
adriamycin induces p53-mediated gene expression and markedly increases the catalytic activity of the enzyme
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hydroxyproline oxidase expression is not increased in rats on a high-fat, carbohydrate-free diet
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in rats, hydroxyproline oxidase activity is maximal in the suckling period
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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the enzyme is a therapeutic target for the treatment of primary hyperoxaluria