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IUBMB Comments Requires Mn2+ 2+ 2+ 2+
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23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
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23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
plant terpenoid
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23,24-dihydrocucurbitacin:NAD(P)+ DELTA23-oxidoreductase
Requires Mn2+. Fe2+ or Zn2+ can replace Mn2+ to some extent.
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cucurbitacin A + NAD(P)H
23,24-dihydrocucurbitacin A + NAD(P)+
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Substrates: very low activity
?
cucurbitacin B + NAD(P)H + H+
23,24-dihydrocucurbitacin B + NAD(P)+
cucurbitacin B + NADH + H+
23,24-dihydrocucurbitacin B + NAD+
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Substrates: -
r
cucurbitacin C + NAD(P)H
23,24-dihydrocucurbitacin C + NAD(P)+
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Substrates: very low activity
?
cucurbitacin D + NAD(P)H
23,24-dihydrocucurbitacin D + NAD(P)+
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Substrates: low activity
?
cucurbitacin E + NAD(P)H
23,24-dihydrocucurbitacin E + NAD(P)+
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Substrates: low activity
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cucurbitacin E beta-D-glucoside + NAD(P)H
23,24-dihydrocucurbitacin E beta-D-glucoside + NAD(P)+
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Substrates: best substrate
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cucurbitacin F + NAD(P)H
23,24-dihydrocucurbitacin F + NAD(P)+
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Substrates: very low activity
?
cucurbitacin B + NAD(P)H + H+
23,24-dihydrocucurbitacin B + NAD(P)+
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Substrates: -
ir
cucurbitacin B + NAD(P)H + H+
23,24-dihydrocucurbitacin B + NAD(P)+
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Substrates: NADPH better electron donor than NADH, reduction is fairly irreversible, approx. 75% of activity with cucurbitacin E beta-D-glucoside
ir
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6.65
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crude enzyme preparation in presence of Zn2+ has a wider pH optimum range than purified enzyme in presence of Mn2+
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5 - 8
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pH 5.0: about 25% of activity at maximum, pH 8.0: about 80% of activity at maximum of crude enzyme preparation in presence of Zn2+, about 10% of activity at maximum of purified enzyme in presence of Mn2+
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var. Green Hubbard
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brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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15000
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2 * 15000, SDS-PAGE
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dimer
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2 * 15000, SDS-PAGE
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50
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5 min, no loss of activity, 10 min, 30% loss of activity
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0-4°C, maleic acid-NaOH buffer, pH 6.6, 100 mM NaCl, 5 weeks
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Schabort, J.C.; Potgieter, D.J.J.; De Villiers, V.
Cucurbitacin B DELTA23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification
Biochim. Biophys. Acta
151
33-46
1968
Cucurbita maxima
brenda
Schabort, J.C.; Potgieter, D.J.J.
Cucurbitacin B DELTA23-reductase from Cucurbita maxima. II. Cofactor requirements, enzyme kinetics, substrate specificity and other characteristics
Biochim. Biophys. Acta
151
47-53
1968
Cucurbita maxima
brenda
Dirr, H.W.; Schabort, J.C.; Weitz, C.
Cucurbitacin DELTA23-reductase from the fruit of Cucurbita maxima var. Green Hubbard. Physicochemical and fluorescence properties and enzyme-ligand interactions
Biochem. J.
233
649-653
1986
Cucurbita maxima
brenda
Schabort, J.C.; Teijema, H.J.
The role of cucurbitacin DELTA23-reductase in the breakdown pathway of toxic bitter principles in Cucurbita maxima
Phytochemistry
7
2107-2110
1968
Cucurbita maxima
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brenda
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