BRENDA - Enzyme Database
show all sequences of 1.3.1.5

The role of cucurbitacin DELTA23-reductase in the breakdown pathway of toxic bitter principles in Cucurbita maxima

Schabort, J.C.; Teijema, H.J.; Phytochemistry 7, 2107-2110 (1968)
No PubMed abstract available

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Cucurbita maxima
-
var. Green Hubbard
-
Reaction
Reaction
Commentary
Organism
Reaction ID
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
plant terpenoid
Cucurbita maxima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cucurbitacin A + NAD(P)H
very low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin A + NAD(P)+
-
-
-
?
cucurbitacin B + NAD(P)H + H+
NADPH better electron donor than NADH, reduction is fairly irreversible, approx. 75% of activity with cucurbitacin E beta-D-glucoside
390834
Cucurbita maxima
23,24-dihydrocucurbitacin B + NAD(P)+
-
-
-
ir
cucurbitacin C + NAD(P)H
very low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin C + NAD(P)+
-
-
-
?
cucurbitacin D + NAD(P)H
low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin D + NAD(P)+
-
-
-
?
cucurbitacin E + NAD(P)H
low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin E + NAD(P)+
-
-
-
?
cucurbitacin E beta-D-glucoside + NAD(P)H
best substrate
390834
Cucurbita maxima
23,24-dihydrocucurbitacin E beta-D-glucoside + NAD(P)+
-
-
-
?
cucurbitacin F + NAD(P)H
very low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin F + NAD(P)+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Cucurbita maxima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Cucurbita maxima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cucurbitacin A + NAD(P)H
very low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin A + NAD(P)+
-
-
-
?
cucurbitacin B + NAD(P)H + H+
NADPH better electron donor than NADH, reduction is fairly irreversible, approx. 75% of activity with cucurbitacin E beta-D-glucoside
390834
Cucurbita maxima
23,24-dihydrocucurbitacin B + NAD(P)+
-
-
-
ir
cucurbitacin C + NAD(P)H
very low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin C + NAD(P)+
-
-
-
?
cucurbitacin D + NAD(P)H
low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin D + NAD(P)+
-
-
-
?
cucurbitacin E + NAD(P)H
low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin E + NAD(P)+
-
-
-
?
cucurbitacin E beta-D-glucoside + NAD(P)H
best substrate
390834
Cucurbita maxima
23,24-dihydrocucurbitacin E beta-D-glucoside + NAD(P)+
-
-
-
?
cucurbitacin F + NAD(P)H
very low activity
390834
Cucurbita maxima
23,24-dihydrocucurbitacin F + NAD(P)+
-
-
-
?
Other publictions for EC 1.3.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390833
Dirr
Cucurbitacin DELTA23-reductase ...
Cucurbita maxima
Biochem. J.
233
649-653
1986
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
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1
-
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1
-
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-
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-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390831
Schabort
Cucurbitacin B DELTA23-reducta ...
Cucurbita maxima
Biochim. Biophys. Acta
151
33-46
1968
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
1
1
1
1
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
390832
Schabort
Cucurbitacin B DELTA23-reducta ...
Cucurbita maxima
Biochim. Biophys. Acta
151
47-53
1968
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-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
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8
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
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-
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1
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-
-
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8
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
390834
Schabort
-
The role of cucurbitacin DELTA ...
Cucurbita maxima
Phytochemistry
7
2107-2110
1968
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-
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1
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1
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7
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1
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1
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7
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