Cucurbitacin DELTA23-reductase from the fruit of Cucurbita maxima var. Green Hubbard. Physicochemical and fluorescence properties and enzyme-ligand interactions

Dirr, H.W.; Schabort, J.C.; Weitz, C.; Biochem. J. 233, 649-653 (1986)

Data extracted from this reference:

Reaction

Commentary

Organism

Reaction ID

23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+

plant terpenoid

Cucurbita maxima

Cofactor

Commentary

Organism

Structure

NAD(P)+

-

Cucurbita maxima

Cofactor

Commentary

Organism

Structure

NAD(P)+

-

Cucurbita maxima

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

390833

Dirr

Cucurbitacin DELTA23-reductase ...

Cucurbita maxima

Biochem. J.

233

649-653

1986

-

-

-

-

-

-

-

-

-

-

2

-

-

1

-

-

-

1

-

1

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390831

Schabort

Cucurbitacin B DELTA23-reducta ...

Cucurbita maxima

Biochim. Biophys. Acta

151

33-46

1968

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

1

1

-

1

1

1

1

-

-

1

-

1

-

1

1

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

1

1

1

-

1

-

1

-

1

1

-

-

-

-

-

-

-

-

390832

Schabort

Cucurbitacin B DELTA23-reducta ...

Cucurbita maxima

Biochim. Biophys. Acta

151

47-53

1968

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

-

1

-

-

-

-

8

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

8

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390834

Schabort

-

The role of cucurbitacin DELTA ...

Cucurbita maxima

Phytochemistry

7

2107-2110

1968

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

7

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

7

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-