Information on EC 1.23.1.3 - (-)-pinoresinol reductase

for references in articles please use BRENDA:EC1.23.1.3
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.23.1.3
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RECOMMENDED NAME
GeneOntology No.
(-)-pinoresinol reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(-)-lariciresinol + NADP+ = (-)-pinoresinol + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
matairesinol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(-)-lariciresinol:NADP+ oxidoreductase
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that usually further reduces the product to (+)-secoisolariciresinol [EC 1.23.1.4, (-)-lariciresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) [1], Linum perenne (perennial flax) [2] and Arabidopsis thaliana (thale cress) [3].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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G2IMF2
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-lariciresinol + NADPH + H+
(+)-secoisolariciresinol + NADP+
show the reaction diagram
(-)-pinoresinol + NADPH + H+
(-)-lariciresinol + NADP+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(-)-pinoresinol + NADPH + H+
(-)-lariciresinol + NADP+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0073 - 0.0126
(-)-pinoresinol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.17 - 19
(-)-pinoresinol
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
G2IMF2;
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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cell layer-specific localization of secoisolariciresinol diglucoside (SDG) in flaxseed coats
Manually annotated by BRENDA team
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M MES, pH 6.2, 0.1 M NaCl, and 0.1 M calcium acetate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-Bind resin affinity column chromatography; His-Bind resin affinity column chromatography
Ni-NTA column chromatography
POROS 20 HQ column chromatography and ADP-agarose column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli B834-DE3 cells
expressed in Escherichia coli BL21 (DE3) cells; expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli Nova Blue cells
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expressed in Escherichia coli strain HB101
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gene LuPLR1, quantitative RT-PCR expression analysis of LuPLR1
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gene pinZ, recombinant expression in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter, pinZ expression causes dynamic metabolic changes in stems, but not in roots and leaves. Accumulation of the glucoside of secoisolariciresinol appears to be elevated in the transgenic plant. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers, recombinant enzyme tissue expression pattern in plant seedlings
G2IMF2;
gene PLR_Lu1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis
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gene PrR1, PrR1 is co-expressed with many characterized genes involved in secondary cell wall biosynthesis, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46
the plasmid is transferred into the disarmed Agrobacter tumefaciens strain GV3101 by triparental mating with Escherichia coli strain HB101 and then introduced into Linum usitatissimum (cv. Barbara) transgenic plants
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the promoter of one pinoresinol-lariciresinol reductase gene LuPLR1 is fused to a beta-glucuronidase (GUS) reporter gene, and the spatiotemporal regulation of LuPLR1 gene expression via this promoter in flaxseed is determined by histochemical and activity assays of GUS, recombinant expression of the promoter construct in transgenic flaxseed via transformation with Agrobacterium tumefaciens, quantitative RT-PCR expression analysis
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression of the PLR1 gene in the seed coat is down-regulated about 3fold when 0.01 mM fluridone is applied
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the expression of the PLR1 gene in the seed coat is up-regulated about 3fold by 0.1 mM exogenous abscisic acid. There is a high mid-maturation expression of PLR1 gene between developmental stage 2 and stage
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information