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show all sequences of 1.23.1.3

Laser microdissection and spatiotemporal pinoresinol-lariciresinol reductase gene expression assign the cell layer-specific accumulation of secoisolariciresinol diglucoside in flaxseed coats

Fang, J.; Ramsay, A.; Renouard, S.; Hano, C.; Lamblin, F.; Chabbert, B.; Mesnard, F.; Schneider, B.; Front. Plant Sci. 7, 1743 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the promoter of one pinoresinol-lariciresinol reductase gene LuPLR1 is fused to a beta-glucuronidase (GUS) reporter gene, and the spatiotemporal regulation of LuPLR1 gene expression via this promoter in flaxseed is determined by histochemical and activity assays of GUS, recombinant expression of the promoter construct in transgenic flaxseed via transformation with Agrobacterium tumefaciens, quantitative RT-PCR expression analysis
Linum usitatissimum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-pinoresinol + NADPH + H+
Linum usitatissimum
-
(-)-lariciresinol + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Linum usitatissimum
Q4R0H9
cv. Barbara
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
no enzyme activity is detected in the inner integument and the embryo regardless of the developmental stage
Linum usitatissimum
-
parenchymal cell
cell layer-specific localization of secoisolariciresinol diglucoside (SDG) in flaxseed coats
Linum usitatissimum
-
seed
spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview
Linum usitatissimum
-
seed coat
the parenchymatous cell layer of the outer integument of flaxseed coats, the enzyme is strongly expressed in the seed coats of developing flaxseed, quantitative RT-PCR expression analysis or recombinant PLR1 promoter construct in transgenic flaxseed
Linum usitatissimum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-pinoresinol + NADPH + H+
-
744996
Linum usitatissimum
(-)-lariciresinol + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Linum usitatissimum
Cloned(Commentary) (protein specific)
Commentary
Organism
the promoter of one pinoresinol-lariciresinol reductase gene LuPLR1 is fused to a beta-glucuronidase (GUS) reporter gene, and the spatiotemporal regulation of LuPLR1 gene expression via this promoter in flaxseed is determined by histochemical and activity assays of GUS, recombinant expression of the promoter construct in transgenic flaxseed via transformation with Agrobacterium tumefaciens, quantitative RT-PCR expression analysis
Linum usitatissimum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Linum usitatissimum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-pinoresinol + NADPH + H+
Linum usitatissimum
-
(-)-lariciresinol + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
no enzyme activity is detected in the inner integument and the embryo regardless of the developmental stage
Linum usitatissimum
-
parenchymal cell
cell layer-specific localization of secoisolariciresinol diglucoside (SDG) in flaxseed coats
Linum usitatissimum
-
seed
spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview
Linum usitatissimum
-
seed coat
the parenchymatous cell layer of the outer integument of flaxseed coats, the enzyme is strongly expressed in the seed coats of developing flaxseed, quantitative RT-PCR expression analysis or recombinant PLR1 promoter construct in transgenic flaxseed
Linum usitatissimum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-pinoresinol + NADPH + H+
-
744996
Linum usitatissimum
(-)-lariciresinol + NADP+
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, proposed biosynthesis pathway of SDG oligomer, overview
Linum usitatissimum
physiological function
the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, responsible for the synthesis of the enantiomer (+)-secoisolariciresinol, spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview
Linum usitatissimum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, proposed biosynthesis pathway of SDG oligomer, overview
Linum usitatissimum
physiological function
the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, responsible for the synthesis of the enantiomer (+)-secoisolariciresinol, spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview
Linum usitatissimum
Other publictions for EC 1.23.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746162
Corbin
Functional characterization o ...
Linum usitatissimum
Planta
246
405-420
2017
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1
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2
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2
2
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744996
Fang
Laser microdissection and spa ...
Linum usitatissimum
Front. Plant Sci.
7
1743
2016
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1
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4
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1
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2
2
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746001
Zhao
Pinoresinol reductase 1 impac ...
Arabidopsis thaliana
Phytochemistry
112
170-178
2015
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-
1
-
1
-
-
-
1
-
-
2
-
4
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2
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2
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1
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1
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2
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3
3
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744143
Tamura
Successful expression of a no ...
Sphingobium sp. SYK-6
Appl. Microbiol. Biotechnol.
98
8165-8177
2014
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-
1
-
-
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2
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1
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2
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1
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1
1
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745630
Renouard
RNAi-mediated pinoresinol lar ...
Linum usitatissimum
J. Plant Physiol.
171
1372-1377
2014
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1
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1
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2
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2
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2
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2
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1
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2
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1
1
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723502
Renouard
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Linum usitatissimum
Planta
235
85-98
2012
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2
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3
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1
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1
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1
1
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3
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1
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2
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2
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723491
Hemmati
Pinoresinol-lariciresinol redu ...
Linum usitatissimum
Planta Med.
76
928-934
2010
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-
-
-
-
-
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-
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3
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3
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1
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1
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1
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3
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1
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722657
Nakatsubo
Characterization of Arabidopsi ...
Arabidopsis thaliana
J. Biol. Chem.
283
15550-15557
2008
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1
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2
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2
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1
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4
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2
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2
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4
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4
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1
2
-
2
2
723492
Hano
Pinoresinol-lariciresinol redu ...
Linum usitatissimum
Planta
224
1291-1301
2006
-
-
1
-
-
-
-
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1
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3
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1
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1
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1
1
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3
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1
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723360
von Heimendahl
Pinoresinol-lariciresinol redu ...
Linum usitatissimum
Phytochemistry
66
1254-1263
2005
-
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1
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1
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722638
Min
Crystal structures of pinoresi ...
Thuja plicata
J. Biol. Chem.
278
50714-50723
2003
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1
1
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2
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1
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2
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1
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1
2
1
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719799
Fujita
Recombinant pinoresinol-larici ...
Thuja plicata
J. Biol. Chem.
274
618-627
1999
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1
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