BRENDA - Enzyme Database show
show all sequences of 1.23.1.3

Pinoresinol reductase 1 impacts lignin distribution during secondary cell wall biosynthesis in Arabidopsis

Zhao, Q.; Zeng, Y.; Yin, Y.; Pu, Y.; Jackson, L.A.; Engle, N.L.; Martin, M.Z.; Tschaplinski, T.J.; Ding, S.Y.; Ragauskas, A.J.; Dixon, R.A.; Phytochemistry 112, 170-178 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene PrR1, PrR1 is co-expressed with many characterized genes involved in secondary cell wall biosynthesis, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
the prr1-1 knock-out mutant SALK_058467 contains a T-DNA insertion within an exonic region of the PrR1 gene. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-pinoresinol + NADPH + H+
Arabidopsis thaliana
-
(-)-lariciresinol + NADP+
-
-
?
additional information
Arabidopsis thaliana
(-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme PrR1, cf. Ec 1.23.1.4
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9FVQ6
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
PrR1 is highly expressed in lignified inflorescence stem tissue
Arabidopsis thaliana
-
seed coat
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-pinoresinol + NADPH + H+
-
746001
Arabidopsis thaliana
(-)-lariciresinol + NADP+
-
-
-
?
additional information
(-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme PrR1, cf. Ec 1.23.1.4
746001
Arabidopsis thaliana
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PrR1, PrR1 is co-expressed with many characterized genes involved in secondary cell wall biosynthesis, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the prr1-1 knock-out mutant SALK_058467 contains a T-DNA insertion within an exonic region of the PrR1 gene. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(-)-pinoresinol + NADPH + H+
Arabidopsis thaliana
-
(-)-lariciresinol + NADP+
-
-
?
additional information
Arabidopsis thaliana
(-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme PrR1, cf. Ec 1.23.1.4
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
PrR1 is highly expressed in lignified inflorescence stem tissue
Arabidopsis thaliana
-
seed coat
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-pinoresinol + NADPH + H+
-
746001
Arabidopsis thaliana
(-)-lariciresinol + NADP+
-
-
-
?
additional information
(-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme PrR1, cf. Ec 1.23.1.4
746001
Arabidopsis thaliana
?
-
-
-
-
General Information
General Information
Commentary
Organism
malfunction
the loss-of-function mutant of PrR1 shows, in addition to elevated levels of pinoresinol, significantly decreased lignin content and a slightly altered lignin structure with lower abundance of cinnamyl alcohol end groups. Stimulated Raman scattering (SRS) microscopy analysis indicates that the lignin content of the prr1-1 loss-of-function mutant is similar to that of wild-type plants in xylem cells, which exhibit a normal phenotype, but is reduced in the fiber cells. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin. The PrR1 isozyme mutation alters the expression level of diverse genes, overview
Arabidopsis thaliana
metabolism
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
Arabidopsis thaliana
physiological function
pinoresinol reductase (PrR) catalyzes the conversion of the lignan pinoresinol to lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. Association of the lignan biosynthetic enzyme encoded by PrR1 with secondary cell wall biosynthesis in fiber cells. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46. PrR1 plays a role in lignan biosynthesis in aerial tissue
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the loss-of-function mutant of PrR1 shows, in addition to elevated levels of pinoresinol, significantly decreased lignin content and a slightly altered lignin structure with lower abundance of cinnamyl alcohol end groups. Stimulated Raman scattering (SRS) microscopy analysis indicates that the lignin content of the prr1-1 loss-of-function mutant is similar to that of wild-type plants in xylem cells, which exhibit a normal phenotype, but is reduced in the fiber cells. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin. The PrR1 isozyme mutation alters the expression level of diverse genes, overview
Arabidopsis thaliana
metabolism
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
Arabidopsis thaliana
physiological function
pinoresinol reductase (PrR) catalyzes the conversion of the lignan pinoresinol to lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. Association of the lignan biosynthetic enzyme encoded by PrR1 with secondary cell wall biosynthesis in fiber cells. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46. PrR1 plays a role in lignan biosynthesis in aerial tissue
Arabidopsis thaliana
Other publictions for EC 1.23.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746162
Corbin
Functional characterization o ...
Linum usitatissimum
Planta
246
405-420
2017
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744996
Fang
Laser microdissection and spa ...
Linum usitatissimum
Front. Plant Sci.
7
1743
2016
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2
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746001
Zhao
Pinoresinol reductase 1 impac ...
Arabidopsis thaliana
Phytochemistry
112
170-178
2015
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1
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1
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3
3
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744143
Tamura
Successful expression of a no ...
Sphingobium sp. SYK-6
Appl. Microbiol. Biotechnol.
98
8165-8177
2014
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1
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745630
Renouard
RNAi-mediated pinoresinol lar ...
Linum usitatissimum
J. Plant Physiol.
171
1372-1377
2014
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1
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1
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1
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723502
Renouard
Abscisic acid regulates pinore ...
Linum usitatissimum
Planta
235
85-98
2012
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1
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2
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2
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723491
Hemmati
Pinoresinol-lariciresinol redu ...
Linum usitatissimum
Planta Med.
76
928-934
2010
-
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3
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3
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722657
Nakatsubo
Characterization of Arabidopsi ...
Arabidopsis thaliana
J. Biol. Chem.
283
15550-15557
2008
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2
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2
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2
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4
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4
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1
2
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2
2
723492
Hano
Pinoresinol-lariciresinol redu ...
Linum usitatissimum
Planta
224
1291-1301
2006
-
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1
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1
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3
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1
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723360
von Heimendahl
Pinoresinol-lariciresinol redu ...
Linum usitatissimum
Phytochemistry
66
1254-1263
2005
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1
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722638
Min
Crystal structures of pinoresi ...
Thuja plicata
J. Biol. Chem.
278
50714-50723
2003
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1
1
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719799
Fujita
Recombinant pinoresinol-larici ...
Thuja plicata
J. Biol. Chem.
274
618-627
1999
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