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Literature summary for 1.23.1.3 extracted from

  • Renouard, S.; Tribalatc, M.A.; Lamblin, F.; Mongelard, G.; Fliniaux, O.; Corbin, C.; Marosevic, D.; Pilard, S.; Demailly, H.; Gutierrez, L.; Hano, C.; Mesnard, F.; Laine, E.
    RNAi-mediated pinoresinol lariciresinol reductase gene silencing in flax (Linum usitatissimum L.) seed coat consequences on lignans and neolignans accumulation (2014), J. Plant Physiol., 171, 1372-1377 .
    View publication on PubMed
Search for more literature for 1.23.1.3

Cloned(Commentary)

Cloned (Comment) Organism
gene PLR_Lu1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis Linum usitatissimum

Protein Variants

Protein Variants Comment Organism
additional information RNAi-mediated pinoresinol lariciresinol reductase gene silencing, via Agrobacterium tumefaciens strain GV301 transformation, in Linum usitatissimum seed coat with consequences on lignans and neolignans accumulation, LuPLR1 transcript level is strongly reduced in the seeds ofPLR-RNAi plants, phenotype, overview Linum usitatissimum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-pinoresinol + NADPH + H+ Linum usitatissimum
-
 (-)-lariciresinol + NADP+
-
 ?
additional information Linum usitatissimum (-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme LuPLR1, cf. EC 1.23.1.4 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Linum usitatissimum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-pinoresinol + NADPH + H+
-
 Linum usitatissimum (-)-lariciresinol + NADP+
-
 ?
additional information (-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme LuPLR1, cf. EC 1.23.1.4 Linum usitatissimum ?
-
 ?

Synonyms

Synonyms Comment Organism
LuPLR1
-
 Linum usitatissimum
More cf. EC 1.23.1.4 Linum usitatissimum
pinoresinol lariciresinol reductase
-
 Linum usitatissimum

General Information

General Information Comment Organism
malfunction RNAi-generated plants exhibit LuPLR1 gene silencing and fail to accumulate secoisolariciresinol diglucoside (SDG). The accumulation of pinoresinol, the substrate of the PLR1 enzyme, in its diglucosylated form (PDG) is increased in transgenic seeds but does not compensate the overall loss of SDG. The monolignol flux is also deviated through the synthesis of 8-5'-linked neolignans dehydrodiconiferyl alcohol glucoside (DCG) and dihydro-dehydrodiconiferyl alcohol glucoside (DDCG) Linum usitatissimum