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Information on EC 1.14.19.20 - DELTA7-sterol 5(6)-desaturase and Organism(s) Arabidopsis thaliana and UniProt Accession Q39208

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IUBMB Comments
This enzyme, found in eukaryotic organisms, catalyses the introduction of a double bond between the C5 and C6 carbons of the B ring of Delta7-sterols, to yield the corresponding Delta5,7-sterols. The enzymes from yeast, plants and vertebrates act on avenasterol, episterol, and lathosterol, respectively. The enzyme is located at the endoplasmic reticulum and is membrane bound.
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Arabidopsis thaliana
UNIPROT: Q39208
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
erg3p, c-5 sterol desaturase, 5-des, erg 3, fvc5sd, lathosterol 5-desaturase, sterol c5-desaturase, sterol-c5-desaturase, des5a, delta7-sterol-c5(6)-desaturase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA7-sterol 5-desaturase
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DELTA7-sterol DELTA5-dehydrogenase
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DELTA7-sterol-C5(6)-desaturase
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5alpha-cholest-7-en-3beta-ol:oxygen DELTA5-oxidoreductase
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-
-
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C-5 sterol desaturase
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-
-
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Delta-7-sterol 5-desaturase
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-
-
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DELTA7-sterol 5-desaturase
-
-
-
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DELTA7-sterol DELTA5-dehydrogenase
-
-
-
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DELTA7-sterol-C-5-desaturase
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DELTA7-sterol-C5-desaturase
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Lathosterol 5-desaturase
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-
-
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lathosterol oxidase
-
-
-
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oxidase, lathosterol
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-
-
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Sterol-C5-desaturase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a DELTA7-sterol + 2 ferrocytochrome b5 + O2 + 2 H+ = a DELTA5,7-sterol + 2 ferricytochrome b5 + 2 H2O
show the reaction diagram
enzyme initiates oxidation by cleavage of the chemically activated C6alpha-H bond, proposed mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
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oxidation
-
-
-
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reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
DELTA7-sterol,ferrocytochrome b5:oxygen oxidoreductase 5,6-dehydrogenating
This enzyme, found in eukaryotic organisms, catalyses the introduction of a double bond between the C5 and C6 carbons of the B ring of Delta7-sterols, to yield the corresponding Delta5,7-sterols. The enzymes from yeast, plants and vertebrates act on avenasterol, episterol, and lathosterol, respectively. The enzyme is located at the endoplasmic reticulum and is membrane bound.
CAS REGISTRY NUMBER
COMMENTARY hide
37255-37-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5alpha-cholest-7-en-3beta-ol + ferrocytochrmoe b5 + H+ + O2
cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + H2O
show the reaction diagram
NADH is more efficient than NADPH
-
-
?
5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + H+ + O2
cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + 2 H2O
show the reaction diagram
NADH is more efficient than NADPH
-
-
?
5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5
ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5
show the reaction diagram
-
-
-
?
DELTA7-avenasterol + O2 + ferrocytochrome b5
DELTA5,7-avenasterol + H2O + ferricytochrome b5
show the reaction diagram
-
-
-
-
?
DELTA7-campesterol + O2 + ferrocytochrome b5
DELTA5,7-campesterol + H2O + ferricytochrome b5
show the reaction diagram
-
-
-
-
?
DELTA7-sitosterol + O2 + ferrocytochrome b5
DELTA5,7-sitosterol + H2O + ferricytochrome b5
show the reaction diagram
-
-
-
-
?
episterol + ferrocytochrome b5 + O2 + H+
ergosta-5,7,24(28)-trien-3beta-ol + ferricytochrome b5 + 2 H2O
show the reaction diagram
-
-
-
-
?
episterol + O2 + ferrocytochrome b5
DELTA5,7-episterol + H2O + ferricytochrome b5
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
NADH is more efficient than NADPH
NADPH
NADH is more efficient than NADPH
cytochrome b5
-
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CN-
0.003 mM, 50% inhibition of recombinant enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032 - 0.244
5alpha-Cholest-7-en-3beta-ol
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SC5D1_ARATH
281
3
33143
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G234A
G234D
H147E
H147L
H151E
H151l
H161E
H161L
H164L
H165L
H203L
H222E
H222L
H238E
H238L
H241L
H242L
K115L
P175A
P175V
P201A
T114I
wild-type activity
T114I |
Km-value for 5alpha-cholest-7-en-3beta-ol is 7.5fold higher than that of wild-type activity
T114S
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in null mutant erg3 of Saccharomyces cerevisiae
expressed in an erg3-defective mutant of Saccharomyces crevisiae
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expressed in Saccharomyces cerevisiae strain erg3
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wild-type enzyme and ste1 mutant enzyme expressed in Sacharomyces cerevisiae erg3 mutant, wild-type enzyme overexpressed in Arabidopsis ste1 mutant
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Husselstein, T.; Schaller, H.; Gachotte, D.; Benveniste, P.
DELTA7-sterol-C5-desaturase: molecular characterization and functional expression of wild-type and mutant alleles
Plant Mol. Biol.
39
891-906
1999
Arabidopsis thaliana, Saccharomyces cerevisiae, Nicotiana tabacum, Zea mays
Manually annotated by BRENDA team
Taton, M.; Husselstein, T.; Benveniste, P.; Rahier, A.
Role of highly conserved residues in the reaction catalyzed by recombinant DELTA7-sterol-C5(6)-desaturase studied by site-directed mutagenesis
Biochemistry
39
701-711
2000
Arabidopsis thaliana (Q39208), Arabidopsis thaliana
Manually annotated by BRENDA team
Rahier, A.
Deuterated DELTA7-cholestenol analogues as mechanistic probes for wild-type and mutated DELTA7-sterol-C5(6)-desaturase
Biochemistry
40
256-267
2001
Arabidopsis thaliana, Zea mays
Manually annotated by BRENDA team
Silvestro, D.; Andersen, T.; Schaller, H.; Jensen, P.
Plant sterol metabolism. delta7-Sterol-C5-desaturase (STE1/DWARF7), delta5,7-sterol-delta7-reductase (DWARF5) and delta24-sterol-delta24-reductase (DIMINUTO/DWARF1) show multiple subcellular localizations in Arabidopsis thaliana (Heynh) L
PLoS ONE
8
e56429
2013
Arabidopsis thaliana
Manually annotated by BRENDA team
Gachotte, D.; Husselstein, T.; Bard, M.; Lacroute, F.; Benveniste, P.
Isolation and characterization of an Arabidopsis thaliana cDNA encoding a DELTA7-sterol-C-5-desaturase by functional complementation of a defective yeast mutant
Plant J.
9
391-398
1996
Arabidopsis thaliana
Manually annotated by BRENDA team