DELTA7-sterol-C5-desaturase: molecular characterization and functional expression of wild-type and mutant alleles

Husselstein, T.; Schaller, H.; Gachotte, D.; Benveniste, P.; Plant Mol. Biol. 39, 891-906 (1999)

Data extracted from this reference:

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

(24Z)-5alpha-stigmast-7,24(241)-dien-3beta-ol + O2 + reduced acceptor

-

390937

Zea mays

(24Z)-stigmasta-5,7,24(241)-trien-3-beta-ol + acceptor + H2O

-

390937

Zea mays

?

5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5

-

390937

Saccharomyces cerevisiae

ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5

-

390937

Saccharomyces cerevisiae

?

5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5

-

390937

Arabidopsis thaliana

ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5

-

390937

Arabidopsis thaliana

?

Cofactor

Commentary

Organism

Structure

NAD(P)H

-

Saccharomyces cerevisiae

NAD(P)H

-

Zea mays

NAD(P)H

-

Arabidopsis thaliana

Cofactor

Commentary

Organism

Structure

NAD(P)H

-

Saccharomyces cerevisiae

NAD(P)H

-

Zea mays

NAD(P)H

-

Arabidopsis thaliana

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

(24Z)-5alpha-stigmast-7,24(241)-dien-3beta-ol + O2 + reduced acceptor

-

390937

Zea mays

(24Z)-stigmasta-5,7,24(241)-trien-3-beta-ol + acceptor + H2O

-

390937

Zea mays

?

5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5

-

390937

Saccharomyces cerevisiae

ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5

-

390937

Saccharomyces cerevisiae

?

5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5

-

390937

Arabidopsis thaliana

ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5

-

390937

Arabidopsis thaliana

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

744054

Rybak

Loss of C-5 sterol desaturase ...

Candida parapsilosis, Candida albicans, Candida parapsilosis CDC317

Antimicrob. Agents Chemother.

61

e00651-17

2017

-

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2

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2

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3

3

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745646

Makarova

Attenuation of UVR-induced vi ...

Mus musculus, Mus musculus C57Bl/6N (JM8A3.N1)

J. Steroid Biochem. Mol. Biol.

171

187-194

2017

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746220

Kamthan

Expression of C-5 sterol desa ...

Flammulina velutipes

PLoS ONE

12

e0173381

2017

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1

1

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728591

Silvestro

Plant sterol metabolism. delta ...

Arabidopsis thaliana

PLoS ONE

8

e56429

2013

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1

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734037

Morio

Amino acid substitutions in th ...

Candida albicans

J. Antimicrob. Chemother.

67

2131-2138

2012

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2

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735282

Poklepovich

The cytochrome b5 dependent C- ...

Tetrahymena thermophila, Tetrahymena thermophila CU428

Steroids

77

1313-1320

2012

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1

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1

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3

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12

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711840

Acimovic

Inhibition of human sterol DEL ...

Homo sapiens

Drug Metab. Dispos.

39

39-46

2011

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713025

Jiang

Quantitative proteomics analys ...

Mus musculus

Mol. Cell. Proteomics

9

1461-1475

2010

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1

1

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697681

Nusblat

C-5(6) sterol desaturase from ...

Tetrahymena thermophila, Tetrahymena thermophila CU428

Eukaryot. Cell

8

1287-1297

2009

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1

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8

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10

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8

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682873

Giera

Lathosterol side chain amidesâ ...

Homo sapiens

Steroids

73

299-308

2008

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15

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2

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1

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3

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8

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23

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684289

Rossi

Clinical phenotype of lathoste ...

Homo sapiens

Am. J. Med. Genet. A

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2371-2381

2007

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671239

Lin

A targeted apoB38.9 mutation i ...

Mus musculus

Am. J. Physiol. Gastrointest. Liver Physiol.

290

G1170-G1176

2006

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Chau

Inactivation of sterol DELTA5, ...

Candida albicans

Antimicrob. Agents Chemother.

49

3646-3651

2005

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2

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655698

Krakowiak

Lathosterolosis: an inborn err ...

Mus musculus

Hum. Mol. Genet.

12

1631-1641

2003

-

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1

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7

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733470

Nishino

-

Nine of ten histidine residues ...

Homo sapiens

Biomed. Res.

24

99-106

2003

-

-

1

-

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1

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390941

Sugawara

Molecular cloning and structur ...

Homo sapiens

Biochim. Biophys. Acta

1533

277-284

2001

-

-

1

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1

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8

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390942

Rahier

Deuterated DELTA7-cholestenol ...

Arabidopsis thaliana, Zea mays

Biochemistry

40

256-267

2001

-

-

1

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2

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8

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390938

Nishi

cDNA cloning of the mammalian ...

Homo sapiens, Mus musculus

Biochim. Biophys. Acta

1490

106-108

2000

-

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2

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2

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8

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390939

Taton

Role of highly conserved resid ...

Arabidopsis thaliana

Biochemistry

39

701-711

2000

-

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2

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46

-

1

12

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5

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390940

Rahier

Biochemistry and site-directed ...

Zea mays

Biochem. Soc. Trans.

28

799-803

2000

-

-

-

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4

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1

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3

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1

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390937

Husselstein

DELTA7-sterol-C5-desaturase: m ...

Arabidopsis thaliana, Saccharomyces cerevisiae, Nicotiana tabacum, Zea mays

Plant Mol. Biol.

39

891-906

1999

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2

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14

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285359

Rahier

The role of cytochrome b5 in 4 ...

Zea mays

Biochem. Biophys. Res. Commun.

236

434-437

1997

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1

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6

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733205

Nishino

Temperature-induced differenti ...

Rattus norvegicus

Arch. Biochem. Biophys.

339

298-304

1997

-

-

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1

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1

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1

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1

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733204

Taton

Plant sterol biosynthesis: Ide ...

Zea mays

Arch. Biochem. Biophys.

325

279-288

1996

-

-

-

-

-

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6

5

1

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1

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1

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-

1

-

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8

-

1

-

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3

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4

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3

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4

6

-

5

1

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1

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8

-

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734924

Gachotte

Isolation and characterization ...

Arabidopsis thaliana

Plant J.

9

391-398

1996

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734923

Gachotte

An Arabidopsis mutant deficien ...

Saccharomyces cerevisiae

Plant J.

8

407-416

1995

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734121

Takakuwa

Properties and kinetics of mem ...

Rattus norvegicus

J. Biol. Chem.

269

27889-27893

1994

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733924

Arthington

Cloning, disruption and sequen ...

Saccharomyces cerevisiae

Gene

102

39-44

1991

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390931

Kawata

Microsomal enzymes of choleste ...

Rattus norvegicus

J. Biol. Chem.

260

6609-6617

1985

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734114

Honjo

Partial purification and chara ...

Rattus norvegicus

J. Biochem.

97

955-959

1985

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390932

Grinstead

Total enzymic synthesis of cho ...

Rattus norvegicus

J. Biol. Chem.

257

13937-13944

1982

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390933

Ishibashi

Intermembrane transfer of 5alp ...

Rattus norvegicus

J. Biol. Chem.

256

12962-12967

1981

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390934

Dempsey

Large scale purification and s ...

Rattus norvegicus

J. Biol. Chem.

256

1867-1873

1981

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390935

Mitropoulos

Effect of triarimol on cholest ...

Rattus norvegicus

Biochem. Biophys. Res. Commun.

71

892-900

1976

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