BRENDA - Enzyme Database
show all sequences of 1.14.19.20

DELTA7-sterol-C5-desaturase: molecular characterization and functional expression of wild-type and mutant alleles

Husselstein, T.; Schaller, H.; Gachotte, D.; Benveniste, P.; Plant Mol. Biol. 39, 891-906 (1999) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Saccharomyces cerevisiae erg3 mutant
Nicotiana tabacum
wild-type enzyme and ste1 mutant enzyme expressed in Sacharomyces cerevisiae erg3 mutant, wild-type enzyme overexpressed in Arabidopsis ste1 mutant
Arabidopsis thaliana
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Nicotiana tabacum
-
-
-
Saccharomyces cerevisiae
-
-
-
Zea mays
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(24Z)-5alpha-stigmast-7,24(241)-dien-3beta-ol + O2 + reduced acceptor
-
390937
Zea mays
(24Z)-stigmasta-5,7,24(241)-trien-3-beta-ol + acceptor + H2O
-
390937
Zea mays
?
5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5
-
390937
Saccharomyces cerevisiae
ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5
-
390937
Saccharomyces cerevisiae
?
5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5
-
390937
Arabidopsis thaliana
ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5
-
390937
Arabidopsis thaliana
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Saccharomyces cerevisiae
NAD(P)H
-
Zea mays
NAD(P)H
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae erg3 mutant
Nicotiana tabacum
wild-type enzyme and ste1 mutant enzyme expressed in Sacharomyces cerevisiae erg3 mutant, wild-type enzyme overexpressed in Arabidopsis ste1 mutant
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Saccharomyces cerevisiae
NAD(P)H
-
Zea mays
NAD(P)H
-
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(24Z)-5alpha-stigmast-7,24(241)-dien-3beta-ol + O2 + reduced acceptor
-
390937
Zea mays
(24Z)-stigmasta-5,7,24(241)-trien-3-beta-ol + acceptor + H2O
-
390937
Zea mays
?
5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5
-
390937
Saccharomyces cerevisiae
ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5
-
390937
Saccharomyces cerevisiae
?
5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5
-
390937
Arabidopsis thaliana
ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5
-
390937
Arabidopsis thaliana
?
Other publictions for EC 1.14.19.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744054
Rybak
Loss of C-5 sterol desaturase ...
Candida parapsilosis, Candida albicans, Candida parapsilosis CDC317
Antimicrob. Agents Chemother.
61
e00651-17
2017
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2
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2
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3
3
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745646
Makarova
Attenuation of UVR-induced vi ...
Mus musculus, Mus musculus C57Bl/6N (JM8A3.N1)
J. Steroid Biochem. Mol. Biol.
171
187-194
2017
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2
2
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746220
Kamthan
Expression of C-5 sterol desa ...
Flammulina velutipes
PLoS ONE
12
e0173381
2017
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1
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1
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7
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1
1
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728591
Silvestro
Plant sterol metabolism. delta ...
Arabidopsis thaliana
PLoS ONE
8
e56429
2013
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1
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734037
Morio
Amino acid substitutions in th ...
Candida albicans
J. Antimicrob. Chemother.
67
2131-2138
2012
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2
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735282
Poklepovich
The cytochrome b5 dependent C- ...
Tetrahymena thermophila, Tetrahymena thermophila CU428
Steroids
77
1313-1320
2012
-
-
1
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1
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3
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12
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2
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711840
Acimovic
Inhibition of human sterol DEL ...
Homo sapiens
Drug Metab. Dispos.
39
39-46
2011
-
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1
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713025
Jiang
Quantitative proteomics analys ...
Mus musculus
Mol. Cell. Proteomics
9
1461-1475
2010
-
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2
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1
1
-
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697681
Nusblat
C-5(6) sterol desaturase from ...
Tetrahymena thermophila, Tetrahymena thermophila CU428
Eukaryot. Cell
8
1287-1297
2009
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1
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8
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10
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682873
Giera
Lathosterol side chain amides ...
Homo sapiens
Steroids
73
299-308
2008
-
-
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-
15
-
2
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1
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3
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1
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23
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1
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684289
Rossi
Clinical phenotype of lathoste ...
Homo sapiens
Am. J. Med. Genet. A
143A
2371-2381
2007
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1
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671239
Lin
A targeted apoB38.9 mutation i ...
Mus musculus
Am. J. Physiol. Gastrointest. Liver Physiol.
290
G1170-G1176
2006
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733105
Chau
Inactivation of sterol DELTA5, ...
Candida albicans
Antimicrob. Agents Chemother.
49
3646-3651
2005
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6
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2
2
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655698
Krakowiak
Lathosterolosis: an inborn err ...
Mus musculus
Hum. Mol. Genet.
12
1631-1641
2003
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7
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733470
Nishino
-
Nine of ten histidine residues ...
Homo sapiens
Biomed. Res.
24
99-106
2003
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1
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1
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2
1
1
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1
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390941
Sugawara
Molecular cloning and structur ...
Homo sapiens
Biochim. Biophys. Acta
1533
277-284
2001
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1
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8
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1
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8
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390942
Rahier
Deuterated DELTA7-cholestenol ...
Arabidopsis thaliana, Zea mays
Biochemistry
40
256-267
2001
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1
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2
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8
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390938
Nishi
cDNA cloning of the mammalian ...
Homo sapiens, Mus musculus
Biochim. Biophys. Acta
1490
106-108
2000
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2
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8
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390939
Taton
Role of highly conserved resid ...
Arabidopsis thaliana
Biochemistry
39
701-711
2000
-
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2
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46
-
1
12
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5
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46
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12
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390940
Rahier
Biochemistry and site-directed ...
Zea mays
Biochem. Soc. Trans.
28
799-803
2000
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4
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390937
Husselstein
DELTA7-sterol-C5-desaturase: m ...
Arabidopsis thaliana, Saccharomyces cerevisiae, Nicotiana tabacum, Zea mays
Plant Mol. Biol.
39
891-906
1999
-
-
2
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-
-
-
-
-
-
-
-
14
-
-
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-
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3
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3
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285359
Rahier
The role of cytochrome b5 in 4 ...
Zea mays
Biochem. Biophys. Res. Commun.
236
434-437
1997
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1
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6
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733205
Nishino
Temperature-induced differenti ...
Rattus norvegicus
Arch. Biochem. Biophys.
339
298-304
1997
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