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EC Tree
IUBMB Comments This cytochrome P-450 (heme-thiolate) enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
The expected taxonomic range for this enzyme is: Papaver
Synonyms
1-hydroxy-N-methylcanadine synthase,
CYP82Y1 , N-methylcanadine 1-hydroxylase, PbCYP82Y1,
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1-hydroxy-N-methylcanadine synthase
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N-methylcanadine 1-hydroxylase
CYP82Y1
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N-methylcanadine 1-hydroxylase
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N-methylcanadine 1-hydroxylase
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(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 = (S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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(S)-N-methylcanadine,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (1-hydroxylating)
This cytochrome P-450 (heme-thiolate) enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
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(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
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(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
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-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
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-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine. The pathway for biosynthesis of noscapine, a potential anticancer compound, from canadine is a 10-gene cluster
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(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
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-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine. The pathway for biosynthesis of noscapine, a potential anticancer compound, from canadine is a 10-gene cluster
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?
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cytochrome P-450
heme-thiolate enzyme
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cytochrome P-450
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heme-thiolate enzyme
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brenda
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UniProt
brenda
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occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
brenda
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
brenda
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brenda
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expression is lower than in Papaver somniferum
brenda
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brenda
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
brenda
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expression is lower than in Papaver somniferum
brenda
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brenda
occurrence of CYP82Y1 transcripts in all plant organs, with the highest levels detected in stems
brenda
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malfunction
suppression of CYP82Y1 using virus-induced gene silencing causes a significant reduction in the levels of noscapine, narcotoline, and a putative downstream secoberbine intermediate and also results in increased accumulation of the upstream pathway intermediates scoulerine, tetrahydrocolumbamine, canadine, and N-methylcanadine
metabolism
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the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
metabolism
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the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
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C82Y1_PAPSO
556
1
62928
Swiss-Prot
Secretory Pathway (Reliability: 2 )
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expression in Saccharomyces cerevisiae
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an SSR (Simple Sequence Repeats) is embedded in the promoter region of the CYP82Y1 gene. Quantitative PCR shows that a variation in the motif repeat number (either a decrease or increase) down-regulates the expression of the CYP82Y1 gene
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Li, Y.; Li, S.; Thodey, K.; Trenchard, I.; Cravens, A.; Smolke, C.
Complete biosynthesis of noscapine and halogenated alkaloids in yeast
Proc. Natl. Acad. Sci. USA
115
E3922-E3931
2018
Papaver somniferum (I3PLR1)
brenda
Dang, T.T.; Facchini, P.J.
CYP82Y1 is N-methylcanadine 1-hydroxylase, a key noscapine biosynthetic enzyme in opium poppy
J. Biol. Chem.
289
2013-2026
2014
Papaver somniferum (I3PLR1), Papaver somniferum
brenda
Abedini, D.; Monfared, S.R.; Abbasi, A.
The effects of promoter variations of the N-methylcanadine 1-hydroxylase (CYP82Y1) gene on the noscapine production in opium poppy
Sci. Rep.
8
4973
2018
Papaver bracteatum, Papaver somniferum (I3PLR1), Papaver somniferum
brenda
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1.14.14.166 (S)-N-methylcanadine 1-hydroxylase
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