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Information on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) and Organism(s) Ovis aries and UniProt Accession P00349

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IUBMB Comments
The enzyme participates in the oxidative branch of the pentose phosphate pathway, whose main purpose is to produce NADPH and pentose for biosynthetic reactions. Highly specific for NADP+. cf. EC 1.1.1.343, phosphogluconate dehydrogenase (NAD+-dependent, decarboxylating).
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Ovis aries
UNIPROT: P00349
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The taxonomic range for the selected organisms is: Ovis aries
The enzyme appears in selected viruses and cellular organisms
Synonyms
6pgd, 6-pgd, 6-p-gluconate dehydrogenase, os6pgdh1, 6-phosphogluconate dehydrogenase (decarboxylating), 6-phospho-d-gluconate dehydrogenase, llpdh, 6-phosphonogluconate dehydrogenase, 6-phosphogluconate dehydrogenase 1, 6-gpd, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-phosphogluconate dehydrogenase
-
6-PGDHase
-
-
6-phospho-D-gluconate dehydrogenase
-
-
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase (decarboxylating)
-
-
-
-
6-phosphogluconic carboxylase
-
-
-
-
6-phosphogluconic dehydrogenase
-
-
-
-
GNTZII
-
-
-
-
phosphogluconic acid dehydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
6-phospho-D-gluconate:NADP+ 2-oxidoreductase (decarboxylating)
The enzyme participates in the oxidative branch of the pentose phosphate pathway, whose main purpose is to produce NADPH and pentose for biosynthetic reactions. Highly specific for NADP+. cf. EC 1.1.1.343, phosphogluconate dehydrogenase (NAD+-dependent, decarboxylating).
CAS REGISTRY NUMBER
COMMENTARY hide
9073-95-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-keto-6-phospho-D-gluconate
D-ribulose 5-phosphate + CO2
show the reaction diagram
via 1,2-enediol intermediate
-
-
?
6,7-dideoxy-7-phosphono-D-glucoheptonate + NADP+
?
show the reaction diagram
-
-
-
?
6-phospho-D-gluconate + NADP+
3-keto-6-phospho-D-gluconate + NADPH + H+
show the reaction diagram
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
2-deoxy-3-oxo 6-phosphogluconate + ?
?
show the reaction diagram
-
no decarboxylation by the liver enzyme
-
-
?
2-deoxy-6-phospho-D-gluconate + NADP+
3-oxo-2-deoxy-6-phosphogluconate + NADPH
show the reaction diagram
-
-
-
-
?
2-deoxy-6-phosphogluconate + NADP+
2-deoxy-3-oxo 6-phosphogluconate + NADPH
show the reaction diagram
3-keto-6-phospho-D-gluconate
D-ribulose 5-phosphate + CO2
show the reaction diagram
-
via 1,2-enediol intermediate
-
-
?
6-O-phosphono-D-mannonic acid + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
3-keto-6-phospho-D-gluconate + NADPH + H+
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
D-ribulose 5-phosphate + CO2 + NADPH
6-phospho-D-gluconate + NADP+
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,5-diphospho-D-ribulose
-
1,5-diphospho-ribitol
-
2-deoxy-6-phosphono-D-gluconate
-
4-phospho-D-erythronate
bio-isosteric analogue
4-phospho-D-erythrose
bio-isosteric analogue
5,6-dideoxy-6-phosphono-D-arabino-hexonate
-
5-deoxy-5-phosphonoD-arabinonate
-
5-phospho-D-arabinonamide
-
5-phospho-D-arabinonate
-
5-phospho-D-arabinonhydrazide
-
5-phospho-D-arabinono-1,4-lactone
-
5-phospho-D-arabinonohydroxamic acid
-
5-phospho-D-arabinose
-
5-phospho-D-ribonate
-
6-deoxy-6-phosphono-D-gluconate
-
6-phospho-D-galactonate
-
6-phosphono-D-mannonate
competitive
NADPH
(2R)-(2-hydroxy-3-phosphonooxypropane-1-sulfinyl)acetic acid
-
-
2'-AMP
-
-
2-deoxy-6-phosphono-D-gluconate
-
-
3'-AMP
-
-
4-phospho-D-erythronamide
-
-
4-phospho-D-erythronate
-
-
4-phospho-D-erythronohydroxamic acid
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
acetic acid (1R,2R)-2-acetoxy-1-(acetylbenzyloxyaminocarbonyl)-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
-
acetic acid (2R,1R)-2-acetoxy-1-hydroxycarbamoyl-3-phosphonooxypropyl ester
-
-
adenosine
-
-
ADP
-
-
citrate
-
-
D-2-deoxyglucose
-
-
D-fructose 1,6-diphosphate
-
-
D-Fructose 1-phosphate
-
-
D-fructose 6-phosphate
-
-
D-glucose 6-phosphate
-
-
diphosphate
-
-
NADP+
-
-
nicotinamide
-
-
oxalate
-
-
oxaloacetate
-
-
p-hydroxymercuribenzoate
-
-
phosphate
-
-
phosphoric acid mono-[(4R,5R)-5-carbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl] ester
-
-
phosphoric acid mono-[(5R,4R)-5-hydroxycarbamoyl-2,2-dimethyl[1,3]dioxolan-4-ylmethyl] ester
-
-
sulfate
-
-
[(2R)-2-acetoxy-3-phosphonooxypropane-1-sulfinyl]acetic acid
-
-
additional information
-
no inhibition by [3-(bis(benzyloxy)phosphoryloxy)-(2R)-2-hydroxypropane-1-sulfinyl]acetic acid, [(2R)-2-acetoxy-3-(bis(benzyloxy)phosphoryloxy)-propane-1-sulfinyl]acetic acid benzyl ester, (2R)-[3-(bis(benzyloxy)phosphoryloxy)-2-(tetrahydropyran-2-yloxy)propane-1-sulfinyl]acetic acid, and acetic acid (1R,2R)-2-acetoxy-1-benzyloxycarbamoyl-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-phosphogluconate
-
bona fide allosteric activator
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011
6,7-dideoxy-7-phosphono-D-glucoheptonate
pH 8.0, 20°C
0.0121 - 3.4
6-phospho-D-gluconate
0.03
6-phosphono-D-gluconate
pH 8.0, 20°C
0.002 - 0.073
NADP+
1.42
2-deoxy-3-oxo-6-phosphogluconate
-
-
1.4 - 1.42
2-deoxy-6-phosphogluconate
0.0069 - 0.313
6-phosphogluconate
0.016
6-phosphono-D-gluconate
-
pH 7.5, 20°C
34
CO2
-
-
0.00571 - 0.03
NADP+
0.00022
NADPH
-
-
0.02
ribulose-5-phosphate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.53
6,7-dideoxy-7-phosphono-D-glucoheptonate
pH 8.0, 20°C
9
6-phosphono-D-gluconate
pH 8.0, 20°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
1,5-diphospho-D-ribulose
pH 8.0, 20°C
0.5
1,5-diphospho-ribitol
pH 8.0, 20°C
0.77
2-deoxy-6-phosphono-D-gluconate
pH 8.0, 20°C
0.011
4-phospho-D-erythronate
pH 8.0, 20°C
1.59
4-phospho-D-erythrose
pH 8.0, 20°C
1
5,6-dideoxy-6-phosphono-D-arabino-hexonate
pH 8.0, 20°C
0.433
5-deoxy-5-phosphonoD-arabinonate
pH 8.0, 20°C
0.0803
5-phospho-D-arabinonamide
pH 8.0, 20°C
0.109
5-phospho-D-arabinonate
pH 8.0, 20°C
0.128
5-phospho-D-arabinonhydrazide
pH 8.0, 20°C
0.147
5-phospho-D-arabinono-1,4-lactone
pH 8.0, 20°C
0.307
5-phospho-D-arabinonohydroxamic acid
pH 8.0, 20°C
0.71
5-phospho-D-arabinose
pH 8.0, 20°C
0.067
5-phospho-D-ribonate
pH 8.0, 20°C
0.0358
6-deoxy-6-phosphono-D-gluconate
pH 8.0, 20°C
0.565
6-phospho-D-galactonate
pH 8.0, 20°C
0.135
6-phosphono-D-mannonate
pH 8.0, 20°C
0.00065 - 0.027
NADPH
0.784
(2R)-(2-hydroxy-3-phosphonooxypropane-1-sulfinyl)acetic acid
-
pH 7.5, 20°C
0.77
2-deoxy-6-phosphono-D-gluconate
-
pH 7.5, 20°C
0.039
4-phospho-D-erythronamide
-
pH 7.5, 20°C
0.011
4-phospho-D-erythronate
-
pH 7.5, 20°C
0.0025
4-phospho-D-erythronohydroxamic acid
-
pH 7.5, 20°C
0.192
acetic acid (1R,2R)-2-acetoxy-1-(acetylbenzyloxyaminocarbonyl)-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
pH 7.5, 20°C
0.00036
acetic acid (2R,1R)-2-acetoxy-1-hydroxycarbamoyl-3-phosphonooxypropyl ester
-
pH 7.5, 20°C
0.714
phosphoric acid mono-[(4R,5R)-5-carbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl] ester
-
pH 7.5, 20°C
0.0011
phosphoric acid mono-[(5R,4R)-5-hydroxycarbamoyl-2,2-dimethyl[1,3]dioxolan-4-ylmethyl] ester
-
pH 7.5, 20°C
0.625
[(2R)-2-acetoxy-3-phosphonooxypropane-1-sulfinyl]acetic acid
-
pH 7.5, 20°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.33
-
-
17.4
-
-
18.7
-
ultrafiltration
19
-
pure enzyme
21.2
-
-
4.7
-
recombinant enzyme
92.3
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
assay at
7.5
-
assay at
7.7
-
low substrate concentrations
8
-
6-phosphogluconate oxidation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
-
5.5 - 9.1
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
assay at
20
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
a 6PGD gene expression pattern in ovines differing in weight is not followed by analogous changes of its enzymatic activity suggesting that other post transcription or post translation regulation is involved
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
6PGD_SHEEP
483
0
52970
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
129000
47000
-
2 * 47000, SDS-PAGE
50000
-
2 * 50000
51000
-
2 * 51000, SDS-PAGE
52000
-
2 * 52000
90000
-
gel filtration, Bio-Gel 200
92000
-
gel filtration, PAGE
94000
95000
-
gel filtration
98000
-
sedimentation equilibrium
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H186A
reduced activity compared to the wild type enzyme
H187A
reduced activity compared to the wild type enzyme
K260A
reduced activity compared to the wild type enzyme
M13C
mutant showing decrease in affinity for NADP+, but not NADPH
M13F
mutant showing decrease in affinity for NADP+, but not NADPH
M13I
mutant showing decrease in affinity for NADP+, but not NADPH
M13Q
mutant showing decrease in affinity for NADP+, but not NADPH
M13V
mutant showing decrease in affinity for NADP+, but not NADPH
R287A
reduced activity compared to the wild type enzyme
R446A
reduced activity compared to the wild type enzyme
S128A
reduced activity compared to the wild type enzyme
T262A
activity similar to the wild type enzyme
Y191A
reduced activity compared to the wild type enzyme
E131A
-
the mutant shows a 2.3fold decrease in turnover number compared to the wild type enzyme
M13C
-
the mutant shows a decrease in turnover number compared to the wild type enzyme
M13Q
-
the mutant shows a 8.1fold decrease in turnover number compared to the wild type enzyme
M13V
-
the mutant shows a decrease in turnover number compared to the wild type enzyme
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
mutant Y191A measured by varying 6PG saturation level of NADP+
669487
9.1
-
unstable at higher pH values
286829
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 50 mM triethanolamine and 2 mM beta-mercaptoethanol at pH 7.5, 1 year, no loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA resin chromatography and ADP-agarose affinity column chromatography
Ni-NTA resin column chromatography and 2?5?ADP agarose chromatography
Ni-NTA column chromatography and ADP-agarose column chromatography
-
recombinant enzyme
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain M15
expressed in Escherichia coli strain M15[pREP4]
amino acid sequence of the sheep liver enzyme deduced from cDNA clones
-
expressed in Escherichia coli M15 cells
-
mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase from sheep liver reverse transcribed and amplified resulting cDNA subcloned into pQE-30 and overproduced in Escherichia coli M15
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Procsal, D.; Holten, D.
Purification and properties of rat liver 6-phosphogluconate dehydrogenase. Activity at normal in vivo concentration
Biochemistry
11
1310-1314
1972
Mus musculus, Ovis aries, Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Manually annotated by BRENDA team
Dyson, J.E.D.; D'Orazio, R.E.; Hanson, W.H.
Sheep liver 6-phosphogluconate dehydrogenase: Isolation procedure and effect of pH, ionic strength, and metal ions on the kinetic parameters
Arch. Biochem. Biophys.
154
623-635
1973
Cyberlindnera jadinii, Ovis aries, Rattus norvegicus
Manually annotated by BRENDA team
Dyson, J.E.D.; D'Orazio, R.E.
Sheep liver 6-phosphogluconate dehydrogenase. Inhibition by nucleoside phosphates and by other metabolic intermediates
J. Biol. Chem.
248
5428-5435
1973
Ovis aries
Manually annotated by BRENDA team
Scott, W.A.; Abramsky, T.
Neurospora 6-phosphogluconate dehydrogenase. I. Purification and characterization of the wild type enzyme
J. Biol. Chem.
248
3535-3544
1973
Cyberlindnera jadinii, Ovis aries, Neurospora crassa
Manually annotated by BRENDA team
Silverberg, M.; Dalziel, K.
Crystalline 6-phosphogluconate dehydrogenase from sheep liver
Eur. J. Biochem.
38
229-238
1973
Cyberlindnera jadinii, Ovis aries, Rattus norvegicus
Manually annotated by BRENDA team
Pearse, B.M.F.; Rosemeyer, M.A.
Human 6-phosphogluconate dehydrogenase. Purification of the erythrocyte enzyme and the influence of ions and NADPH on its activity
Eur. J. Biochem.
42
213-223
1974
Ovis aries, Homo sapiens
Manually annotated by BRENDA team
Pearse, B.M.F.; Rosemeyer, M.A.
The molecular weight and subunit structure of human erythrocyte 6-phosphogluconate dehydrogenase
Eur. J. Biochem.
42
225-235
1974
Cyberlindnera jadinii, Drosophila sp. (in: flies), Ovis aries, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Silverberg, M.; Dalziel, K.
6-Phospho-D-gluconate dehydrogenase from sheep liver
Methods Enzymol.
41
214-220
1975
Ovis aries, Rattus norvegicus
Manually annotated by BRENDA team
Silverberg, M.; Dalziel, K.
Fluorescence studies of coenzyme binding to 6-phosphogluconate dehydrogenase
Arch. Biochem. Biophys.
168
646-651
1975
Ovis aries
Manually annotated by BRENDA team
Betts, S.A.; Mayer, R.J.
Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland
Biochem. J.
151
263-270
1975
Cyberlindnera jadinii, Oryctolagus cuniculus, Escherichia coli, Ovis aries, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Toews, M.L.; Kanji, M.I.; Carper, R.W.
6-Phosphogluconate dehydrogenase. Purification and kinetics
J. Biol. Chem.
251
7127-7131
1976
Cyberlindnera jadinii, Ovis aries, Homo sapiens, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Adams, M.J.; Helliwell, J.R.; Bugg, C.E.
Structure of 6-phosphogluconate dehydrogenase from sheep liver at 6 A resolution
J. Mol. Biol.
112
183-197
1977
Cyberlindnera jadinii, Ovis aries
Manually annotated by BRENDA team
Rippa, M.; Signorini, M.; Bellini, T.; Dallocchio, F.
The active site of 6-phosphogluconate dehydrogenase. A phosphate binding site and its surroundings
Arch. Biochem. Biophys.
189
516-523
1978
Ovis aries
Manually annotated by BRENDA team
Abdallah, M.A.; Adams, M.J.; Archibald, I.G.; Bielmann, J.F.; Helliwell, J.R.; Jenkins, S.E.
Binding of coenzyme and substrate and coenzyme analogues to 6-phosphogluconate dehydrogenase from sheep liver
Eur. J. Biochem.
98
121-130
1979
Ovis aries
Manually annotated by BRENDA team
Wong, C.H.; Pollak, A.; McCurry, S.D.; Sue, J.M.; Knowles, J.R.; Whitesides, G.M.
Synthesis of ribulose 1,5-bisphosphate: Routes from glucose-6-phosphate (via 6-phosphogluconate) and from adenosine monophosphate (via ribose 5-phosphate)
Methods Enzymol.
89
108-121
1982
Cyberlindnera jadinii, Ovis aries
-
Manually annotated by BRENDA team
Carne, A.
Purification by dye-ligand chromatography of an NADP-dependent enzyme, 6-phosphogluconate dehydrogenase from lamb's liver
Anal. Biochem.
121
227-229
1982
Geobacillus stearothermophilus, Ovis aries
Manually annotated by BRENDA team
Adams, M.J.; Archibald, I.G.; Bugg, C.E.; Carne, A.; Gover, S.; Helliwell, J.R.; Pickersgill, R.W.; White, S.W.
The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6 A resolution
EMBO J.
6
1009-1014
1983
Cyberlindnera jadinii, Ovis aries
Manually annotated by BRENDA team
Dallocchio, F.; Matteuzzi, M.; Bellini, T.
Half-site reactivity in 6-phosphogluconate dehydrogenase from human erythrocytes
Biochem. J.
227
305-310
1985
Cyberlindnera jadinii, Ovis aries, Homo sapiens
Manually annotated by BRENDA team
Topham, C.M.; Dalziel, K.
Chemical modification of sheep-liver 6-phosphogluconate dehydrogenase by diethylpyrocarbonate
Eur. J. Biochem.
155
87-94
1986
Cyberlindnera jadinii, Ovis aries
Manually annotated by BRENDA team
Topham, C.M.; Matthews, B.; Dalziel, K.
Kinetic studies of 6-phosphogluconate dehydrogenase from sheep liver
Eur. J. Biochem.
156
555-567
1986
Cyberlindnera jadinii, Ovis aries
Manually annotated by BRENDA team
Hanau, S.; Dallocchio, F.; Rippa, M.
NADPH activates a decarboxylation reaction catalyzed by lamb liver 6-phosphogluconate dehydrogenase
Biochim. Biophys. Acta
1122
273-277
1992
Cyberlindnera jadinii, Ovis aries, Homo sapiens
Manually annotated by BRENDA team
Berdis, A.J.; Cook, P.F.
Overall kinetic mechanism of 6-phosphogluconate dehydrogenase from Candida utilis
Biochemistry
32
2036-2040
1993
Leuconostoc mesenteroides, Cyberlindnera jadinii, Haemophilus influenzae, Ovis aries, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Berdis, A.J.; Cook, P.F.
The 2'-phosphate of NADP is critical for optimum productive binding to 6-phosphogluconate dehydrogenase from Candida utilis
Arch. Biochem. Biophys.
305
551-558
1993
Cyberlindnera jadinii, Ovis aries
Manually annotated by BRENDA team
Price, N.E.; Cook, P.F.
Kinetic and chemical mechanisms of the sheep liver 6-phosphogluconate dehydrogenase
Arch. Biochem. Biophys.
336
215-223
1996
Ovis aries
Manually annotated by BRENDA team
Phillips, C.; Dohnalek, J.; Barrett, M.P.; Adams, M.J.
A 2.8 A resolution structure of 6-phosphogluconate dehydrogenase from the protozoan parasite Trypanosoma brucei: Comparison with the sheep enzyme accounts for differences in activity with coenzyme and substrate analogues
J. Mol. Biol.
25
667-681
1998
Ovis aries, Trypanosoma brucei (P31072)
Manually annotated by BRENDA team
Tsai, C.S.; Chen, Q.
Purification and kinetic characterization of 6-phosphogluconate dehydrogenase from Schizosaccharomyces pombe
Biochem. Cell Biol.
76
637-644
1998
Gluconobacter oxydans, Leuconostoc mesenteroides, Cyberlindnera jadinii, Dicentrarchus labrax, Ovis aries, Neurospora crassa, Pseudomonas sp., Salmonella enterica subsp. enterica serovar Typhimurium, Schizosaccharomyces pombe
Manually annotated by BRENDA team
Chooback, L.; Price, N.E.; Karsten, W.E.; Nelson, J.; Sundstrom, P.; Cook, P.F.
Cloning, expression, purification, and characterization of the 6-phosphogluconate dehydrogenase from sheep liver
Protein Expr. Purif.
13
251-258
1998
Cyberlindnera jadinii, Escherichia coli, Ovis aries, Escherichia coli JM109 M15
Manually annotated by BRENDA team
Rippa, M.; Giovannini, P.P.; Barrett, M.P.; Dallocchio, F.; Hanau, S.
6-Phosphogluconate dehydrogenase: The mechanism of action investigated by a comparison of the enzyme from different species
Biochim. Biophys. Acta
1429
83-92
1998
Cyberlindnera jadinii, Ovis aries, Homo sapiens, Trypanosoma brucei
Manually annotated by BRENDA team
Moritz, B.; Striegel, K.; de Graaf, A.A.; Sahm, H.
Kinetic properties of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Corynebacterium glutamicum and their application for predicting pentose phosphate pathway flux in vivo
Eur. J. Biochem.
267
3442-3452
2000
Acer pseudoplatanus, Gluconobacter oxydans, Corynebacterium glutamicum, [Brevibacterium] flavum, Saccharomyces cerevisiae, [Candida] boidinii, Corynebacterium glutamicum ssp. flavum, Escherichia coli, Ovis aries, Pseudomonas sp., Pseudomonas sp. C
Manually annotated by BRENDA team
Kato, N.; Sahm, H.; Schutte, H.; Wagner, F.
Purification and properties of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from a methanol-utilizing yeast, Candida boidinii
Biochim. Biophys. Acta
566
1-11
1979
Saccharomyces cerevisiae, [Candida] boidinii, Ovis aries
Manually annotated by BRENDA team
Pasti, C.; Rinaldi, E.; Cervellati, C.; Dallocchio, F.; Hardre, R.; Salmon, L.; Hanau, S.
Sugar derivatives as new 6-phosphogluconate dehydrogenase inhibitors selective for the parasite Trypanosoma brucei
Bioorg. Med. Chem.
11
1207-1214
2003
Ovis aries (P00349), Trypanosoma brucei (P31072)
Manually annotated by BRENDA team
Dardonville, C.; Rinaldi, E.; Barrett, M.P.; Brun, R.; Gilbert, I.H.; Hanau, S.
Selective inhibition of Trypanosoma brucei 6-phosphogluconate dehydrogenase by high-energy intermediate and transition-state analogues
J. Med. Chem.
47
3427-3437
2004
Ovis aries, Leishmania donovani, Plasmodium falciparum, Trypanosoma brucei, Trypanosoma cruzi
Manually annotated by BRENDA team
Cervellati, C.; Dallocchio, F.; Bergamini, C.M.; Cook, P.F.
Role of methionine-13 in the catalytic mechanism of 6-phosphogluconate dehydrogenase from sheep liver
Biochemistry
44
2432-2440
2005
Ovis aries (P00349)
Manually annotated by BRENDA team
Li, L.; Zhang, L.; Cook, P.F.
Role of the S128, H186, and N187 triad in substrate binding and decarboxylation in the sheep liver 6-phosphogluconate dehydrogenase reaction
Biochemistry
45
12680-12686
2006
Ovis aries (P00349)
Manually annotated by BRENDA team
Li, L.; Dworkowski, F.S.; Cook, P.F.
Importance in catalysis of the 6-phosphate-binding site of 6-phosphogluconate in sheep liver 6-phosphogluconate dehydrogenase
J. Biol. Chem.
281
25568-25576
2006
Ovis aries (P00349)
Manually annotated by BRENDA team
Wang, J.; Li, S.
Catalytic mechanism of 6-phosphogluconate dehydrogenase: a theoretical investigation
J. Phys. Chem. B
110
7029-7035
2006
Ovis aries (P00349)
Manually annotated by BRENDA team
Cervellati, C.; Li, L.; Andi, B.; Guariento, A.; Dallocchio, F.; Cook, P.F.
Proper orientation of the nicotinamide ring of NADP is important for the precatalytic conformational change in the 6-phosphogluconate dehydrogenase reaction
Biochemistry
47
1862-1870
2008
Ovis aries
Manually annotated by BRENDA team
Laliotis, G.P.; Trivizaki, S.; Bizelis, I.; Charismiadou, M.A.; Rogdakis, E.
Enzymatic and mRNA transcript response of ovine 6-phosphogluconate dehydrogenase (6PGD) in respect to different weights from weaning to four months of age
Anim. Biotechnol.
21
149-155
2010
Ovis aries
Manually annotated by BRENDA team