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Information on EC 1.1.1.27 - L-lactate dehydrogenase and Organism(s) Lactiplantibacillus plantarum and UniProt Accession P56512

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IUBMB Comments
Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
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This record set is specific for:
Lactiplantibacillus plantarum
UNIPROT: P56512
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The taxonomic range for the selected organisms is: Lactiplantibacillus plantarum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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(S)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+
+
=
+
+
Synonyms
lactate dehydrogenase, lactic dehydrogenase, ldh-a, lactate dehydrogenase a, l-lactate dehydrogenase, ldh-5, lactic acid dehydrogenase, ldh-1, pfldh, alpha-hydroxybutyrate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenase, lactate
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Epsilon crystallin
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epsilon-crystallin
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Immunogenic protein p36
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L(+)-nLDH
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-
-
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L-(+)-lactate dehydrogenase
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L-lactic acid dehydrogenase
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L-lactic dehydrogenase
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L-LDH
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lactate dehydrogenase
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lactate dehydrogenase NAD-dependent
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lactic acid dehydrogenase
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lactic dehydrogenase
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LDH
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NAD-lactate dehydrogenase
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proteins, specific or class, anoxic stress response, p34
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
(S)-lactate:NAD+ oxidoreductase
Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-60-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pyruvate + NADH
L-lactate + NAD+
show the reaction diagram
in the cell lysate of the host strain, L-LDH activity is hardly detectable during cultivation. As a consequence of transformation, the ratio between D- and L-isomers is changed due to the increment of L-lactate and the decrement of D-lactate, but there are no significant differences in total lactate concentration between the host and transformant cells. In the transformant harboring pLC18lld, L-LDH activity is detected during the entire cultivation period. L-LDH activity increases rapidly to the maximum level (0.18 U/mg protein) between the early- and mid-exponential growth phases (0-8 h), and decreases thereafter during the stationary phase
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-
?
(S)-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
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-
-
-
r
pyruvate + NADH + H+
(S)-lactate + NAD+
show the reaction diagram
additional information
?
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
fructose 1,6-diphosphate
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L-lactate dehydrogenase form which is not activated by fructose 1,6-diphosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
reduction of pyruvate
7.5
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oxidation of lactate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Leuconostoc citreum using a shuttle vector pLeuCM, change of the pyruvate carbon flux in Leuconostoc citreum from D-lactate into L-lactate by heterologous expression of L-lactate dehydrogenase
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
production of phenyllactic acid from L-Phe by recombinant Escherichia coli coexpressing L-phenylalanine oxidase and L-lactate dehydrogenase. At optimal conditions (L-Phe 6 g/l, pH 7.5, 35°C, CDW 24.5 g/l and 200 rpm), the recombinant strain produces 1.62 g L-phenylalanine/l with a conversion of 28% from L-Phe
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Garvie, E.I.
Bacterial lactate dehydrogenases
Microbiol. Rev.
44
106-139
1980
Acholeplasma laidlawii, Actinomyces viscosus, [Bacillus] caldolyticus, Bacillus subtilis, Bifidobacterium bifidum, Butyrivibrio fibrisolvens, Enterococcus faecalis, Enterococcus faecium, Lactobacillus acidophilus, Lactiplantibacillus plantarum, Levilactobacillus brevis, Lentilactobacillus buchneri, Lacticaseibacillus casei, Limosilactobacillus fermentum, Weissella confusa, Latilactobacillus curvatus, Lactobacillus helveticus, Lactobacillus mesenteroides, Ligilactobacillus salivarius, Lactobacillus vermiforme, Weissella viridescens, Lactococcus lactis, Lactococcus raffinolactis, Staphylococcus aureus, Mycoplasma sp., Pediococcus acidilactici, Pediococcus damnosus, Lapidilactobacillus dextrinicus, Tetragenococcus halophilus, Pediococcus inopinatus, Pediococcus pentosaceus, Rothia dentocariosa, Selenomonas ruminantium, Sporosarcina ureae, Staphylococcus epidermidis, Streptococcus acidominimus, Streptococcus agalactiae, Streptococcus equinus, Lactococcus cremoris, Streptococcus milleri, Streptococcus mitior, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus thermophilus, Streptococcus uberis
Manually annotated by BRENDA team
Hensel, R.; Mayr, U.; Fujiki, H.; Kandler, O.
Comparative studies of lactate dehydrogenases in lactic acid bacteria. Amino-acid composition of an active-site region and chemical properties of the L-lactate dehydrogenase of Lactobacillus casei, Lactobacillus curvatus, Lactobacillus plantarum, and Lactobacillus acidophilus
Eur. J. Biochem.
80
83-92
1977
Lactobacillus acidophilus, Lactiplantibacillus plantarum, Lacticaseibacillus casei, Latilactobacillus curvatus
Manually annotated by BRENDA team
Jin, Q.; Jung, J.Y.; Kim, Y.J.; Eom, H.J.; Kim, S.Y.; Kim, T.J.; Han, N.S.
Production of l-lactate in Leuconostoc citreum via heterologous expression of l-lactate dehydrogenase gene
J. Biotechnol.
144
160-164
2009
Lactiplantibacillus plantarum (P56512), Lactiplantibacillus plantarum
Manually annotated by BRENDA team
Zhang, J.; Li, X.
Novel strategy for phenyllactic acid biosynthesis from phenylalanine by whole cell recombinant Escherichia coli coexpressing L-phenylalanine oxidase and L-lactate dehydrogenase
Biotechnol. Lett.
40
165-171
2018
Lactiplantibacillus plantarum (P59390), Lactiplantibacillus plantarum ATCC BAA-793 (P59390)
Manually annotated by BRENDA team