Information on EC 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase

29454% relative activity compared to acetophenone

ethyl acetoacetate + NADPH

? + NADP+

706142

ethyl acetoacetate + NADPH

ethyl 3-hydroxybutyrate + NADP+

ethylbenzoylacetate + NADPH

? + NADP+

892% relative activity compared to acetophenone

methyl acetoacetate + NADPH

? + NADP+

is not a good substrate as ethyl acetoacetate

propiophenone + NADPH

? + NADP+

123% relative activity compared to acetophenone

propyl acetoacetate + NADPH

? + NADP+

as substrate it is limited to its weak solubility

additional information

23 entries

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(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

3-oxoacyl-[acyl-carrier protein] + NADPH + H+

Pseudomonas putida

A5HIF6

beta-oxidation pathway

693287

3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

8 entries

3-oxoacyl-[acyl-carrier protein] + NADPH

3-hydroxyacyl-[acyl-carrier protein] + NADP+

3-oxoacyl-[acyl-carrier protein] + NADPH + H+

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

Vibrio cholerae

740630

a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+

acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+

acetoacetyl-CoA + NADPH + H+

3-hydroxybutyryl-CoA + NADP+

beta-ketoacyl-[acyl-carrier protein] + NADPH

beta-hydroxyacyl-[acyl-carrier protein] + NADP+

beta-ketoacyl-[acyl-carrier protein] + NADPH + H+

beta-hydroxyacyl-[acyl-carrier protein] + NADP+

additional information

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NADH

NADP+

NADPH

33 entries

additional information

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additional information

the enzyme activity is maximal at a ionic strength of 325 mM

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(E)-1-(4-methylpiperidin-1-yl)-3-phenylprop-2-en-1-one

80% residual activity

(E)-2-nitrophenyl cinnamate

(E)-3-phenoxybenzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

61% residual activity

(E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

(E)-benzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

71% residual activity

(E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

69% residual activity

1,2,3,4,6-penta-O-galloyl-beta-D-glucose

4 entries

3-hydroxybutyryl-CoA

4-acetoxyanthecotulide

Plasmodium falciparum K1

most active linear sesquiterpene lactone from Anthemis auriculata against FabG

4-hydroxyanthecotulide

Plasmodium falciparum K1

linear sesquiterpene lactone from Anthemis auriculata

Acyl carrier protein

Lactococcus lactis

beta-ketobutyryl-CoA reductase activity of FabG1 is inhibited, activity of FabG2 is unaffected

654725

acyl-carrier protein

inhibition of wild-type with increasing concentrations

anthecotulide

Plasmodium falciparum K1

linear sesquiterpene lactone from Anthemis auriculata

Bithionol

over 75% inhibition at 0.02 mM, IC50: 0.010 mM

bromochlorophen

an anthelmintic agent, over 75% inhibition at 0.02 mM, IC50: 0.0154 mM

chlorogenic acid

IC50: 0.881-0.948 mM

669013

di-resorcinol sulfide

over 75% inhibition at 0.02 mM, IC50: 0.0038 mM

DL-3-hydroxybutyryl-CoA

competitive product inhibition

epigallocatechin gallate

grape skin extract

Gallus gallus

inhibits the overall reaction and beta-ketoacyl reductase reaction of FAS with IC50 values of 4.61 microg/ml and 20.3 microg/ml, respectively. Inhibits the overall reaction of FAS competitively with acetyl-CoA, noncompetitively with malonyl-CoA and in a mixed manner with NADPH

735850

Guanidinium chloride

FabG is fully unfolded at 4 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. In presence of NADPH, there is no stabilization of FabG in case of equilibrium unfolding with guanidinium chloride

706639

Hexachlorophene

an anthelmintic and antimicrobial agent, over 75% inhibition at 0.02 mM, IC50: 0.002 mM

kaempferol

synthetic construct

704843

N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide

69% residual activity

NADP+

p-chloromercuribenzoate

Phenylglyoxal

quercetin

resveratrol

inhibits the overall reaction and beta-ketoacyl reductase reaction of FAS with IC50 values of 11.1 microg/ml and 21.9 microg/ml, respectively. In 3 T3-L1 preadipocytes, resveratrol reduces lipid accumulation remarkably

735850

RNAi

Arabidopsis thaliana

suppresses KCR activity, which results in a reduction of cuticular wax load and affects very-long-chain fatty acid composition of sphingolipids, seed triacylglycerols, and root glycerolipids. RNAi-suppressed Arabidopsis KCR1 plants display fused vegetative and reproductive organs, an abnormal root morphology and have abnormal trichome and epidermal cell morphology

700827

Tannic acid

16 entries

Urea

FabG is fully unfolded at 6 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. Two states in the reversible unfolding process of FabG in presence of NADPH, one is an activity-enhanced state and the other, an inactive state in case of equilibrium unfolding with urea

706639

additional information

24 entries

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additional information

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89.2

1,1,1-trifluoroacetone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

5.72

2',3',4',5',6'-pentafluoroacetophenone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

7.38

3'-fluoroacetophenone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

14.34

4'-chloroacetophenone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

12.29

4'-fluoroacetophenone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.0057 - 2.2

acetoacetyl-CoA

21 entries

acetoacetyl-N-acetylcysteamine

285674

0.003 - 0.017

acetoacetyl-[acyl-carrier protein]

5 entries

46.92

acetophenone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

2.71

alpha-chloroacetophenone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.29 - 4.06

beta-ketobutyryl-CoA

8.31

ethyl 4-chloroacetoacetate

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.127

ethyl acetoacetate

1.92

ethylbenzoylacetate

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.035

N-(2-sulfanylethyl)acetamide

Brassica napus

285673

0.29

NADP+

O54438;

with 3-hydroxydecanoyl-N-acetylcysteamine as cosubstrate, pH and temperature not specified in the publication

739774

0.0093 - 0.617

NADPH

21 entries

6.03

propiophenone

with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

additional information

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0.014 - 361

acetoacetyl-CoA

11 entries

0.3

ethyl acetoacetate

0.59 - 361

NADPH

15 entries

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15200 - 26500

acetoacetyl-CoA

585 - 862

NADPH

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0.0102

3-hydroxybutyryl-CoA

pH 6.8, 25°C, recombinant enzyme

additional information

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0.45

(E)-2-nitrophenyl cinnamate

Escherichia coli;

P0AEK2

pH 7.0

0.18

(E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli;

P0AEK2

pH 7.0

0.18

(E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate

Escherichia coli;

P0AEK2

pH 7.0

0.5

4-acetoxyanthecotulide

Plasmodium falciparum K1;

pH 7.0, 25°C

0.75

4-hydroxyanthecotulide

Plasmodium falciparum K1;

pH 7.0, 25°C

anthecotulide

Plasmodium falciparum K1;

pH 7.0, 25°C

0.01

Bithionol

Plasmodium falciparum;

Q86RB1

over 75% inhibition at 0.02 mM, IC50: 0.010 mM

0.0154

bromochlorophen

Plasmodium falciparum;

Q86RB1

an anthelmintic agent, over 75% inhibition at 0.02 mM, IC50: 0.0154 mM

0.881 - 0.948

chlorogenic acid

Escherichia coli;

IC50: 0.881-0.948 mM

669013

0.0038

di-resorcinol sulfide

Plasmodium falciparum;

Q86RB1

over 75% inhibition at 0.02 mM, IC50: 0.0038 mM

0.00554 - 0.1223

epigallocatechin gallate

0.002

Hexachlorophene

Plasmodium falciparum;

Q86RB1

an anthelmintic and antimicrobial agent, over 75% inhibition at 0.02 mM, IC50: 0.002 mM

0.0212

kaempferol

synthetic construct;

pH 7.0

704843

0.215

N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide

Escherichia coli;

P0AEK2

pH 7.0

0.3048

quercetin

synthetic construct;

pH 7.0

704843

0.00078

Tannic acid

Escherichia coli;

706142

additional information

grape skin extract

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0.0017 - 0.0018

recombinant refolded enzyme

670774

0.0019

recombinant soluble enzyme

0.0032

0.005

0.0132

0.0168

0.0189

0.0192

0.0197

0.02

with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.0366

0.06

mutant enzyme K173A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

0.08

with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.13

with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0; with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.15

with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.16

with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

0.44

mutant enzyme R34A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C; mutant enzyme R34A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C

0.46

mutant enzyme K152A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

0.5

mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C

0.65

mutant enzyme Y169A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

0.85

mutant enzyme Y169A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

1.03

with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

1.16

with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

1.2

mutant enzyme K173A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

1.3

mutant enzyme K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

1.6

mutant enzyme R168E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

1.7

mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

2.4

mutant enzyme K173A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C

2.45

with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

2.6

wild type enzyme, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

mutant R187A/R230A, acyl-carrier protein-dependent spectroscopic assay

3.1

mutant enzyme Y169A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

3.5

wild type enzyme, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C

3.93

with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

mutant R230E, acyl-carrier protein-dependent spectroscopic assay

4.3

mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C

4.5

mutant enzyme K173A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

5.4

mutant enzyme D42A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C

5.5

mutant enzyme K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C

6.1

mutant R230A, acyl-carrier protein-dependent spectroscopic assay

6.3

6.9

mutant R187A, acyl-carrier protein-dependent spectroscopic assay

7.3

mutant enzyme D42A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

7.9

mutant enzyme R168E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

7.95

8.4

mutant enzyme Q126E/R168E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

14.8

mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

15.7

mutant enzyme K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

17.4

mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

19.3

mutant enzyme K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

mutant enzyme D42A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C

20.5

wild type enzyme, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

20.7

mutant enzyme R34A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

20.8

wild type enzyme, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

mutant enzyme R34A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

21.7

mutant enzyme R168E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

mutant enzyme K152A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

25.5

mutant enzyme K152A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

31.8

mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C

38.29

with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

42.6

mutant R187E/R230E, acyl-carrier protein-independent spectroscopic assay

45.6

mutant R187A/R230A, acyl-carrier protein-independent spectroscopic assay

54.2

mutant R230E, acyl-carrier protein-independent spectroscopic assay

54.5

mutant R187E, acyl-carrier protein-independent spectroscopic assay

55.3

mutant R230A, acyl-carrier protein-independent spectroscopic assay

57.9

mutant R187A, acyl-carrier protein-independent spectroscopic assay

59.8

70.6

wild-type, acyl-carrier protein-dependent spectroscopic assay

74.6

mutant enzyme D42A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C

280

acetoacetyl-N-acetylcysteamine

450

acetoacetyl-CoA

530

acetoacteyl-ACP

3839

recombinant protein, cosubstrate NADH, pH 7.0, phosphate buffer

4930

purified enzyme

9401

purified enzyme, protein determination by amino acid analysis

285674

83140

Cuphea lanceolata

cosubstrate NADPH, pH 8.35, carbonate buffer

285679

136000

Brassica napus

purified enzyme, protein determination by calculation from absorption at 280 nm

285673

180400

Cuphea lanceolata

recombinant protein, cosubstrate NADPH, pH 7.0, phosphate buffer

285679

additional information

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5.7 - 6.2

6 - 7

6.5

6.8

7.5

assay at

670774

7.6

4 entries

additional information

increase in alpha-secondary deuterium kinetic isotope effect values measured at pH 10 as compared to those obtained at pH 7 points to isotope- and pH-sensitive steps occurring concomitantly

684702

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4 - 10

pH 4: about 60% of maximal activity, pH 10: about 75% of maximal activity

5.5 - 8.5

about 50% of activity maximum at pH 5.5 and 8.5

285678

5.5 - 9.5

pH profile, detailed mechanistic pH-dependence analysis, overview

5.8 - 10

pH profile, pH-dependence analysis, overview

667708

6 - 8.7

Spinacia oleracea

both reaction directions

285668

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assay at

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15 - 37

temperature profile

667708

30 - 55

30°C: about 55% of maximal activity, 55°C: about 65% of maximal activity

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5.9

Pseudomonas putida

A5HIF6;

predicted

693287

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cell culture

Pseudomonas putida

A5HIF6;

strain KCTC1639

KCR1 and KCR2

KCR1 and KCR2 present in inflorescence stem

285670, 285674, 285678

KCR1 and KCR2

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endoplasmic reticulum

KCR1 and KCR2

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4nbt, download, 3D-view

SCOPe (4nbt)

CATH (4nbt)

A9NFJ2

Acholeplasma laidlawii (strain PG-8A);

5b1y, download, 3D-view

SCOPe (5b1y)

CATH (5b1y)

Q9Y8Y1

Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1);

Aquifex aeolicus (strain VF5);

2uvd, download, 3D-view

SCOPe (2uvd)

CATH (2uvd)

A0A1S0QWK0

Bacillus anthracis;

Bacillus subtilis (strain 168);

1edo, download, 3D-view

SCOPe (1edo)

CATH (1edo)

Q93X62

Brassica napus;

Brucella abortus (strain 2308);

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094);

4o6v, download, 3D-view

SCOPe (4o6v)

CATH (4o6v)

B0CL43

Brucella suis (strain ATCC 23445 / NCTC 10510);

Burkholderia multivorans (strain ATCC 17616 / 249);

3ftp, download, 3D-view

SCOPe (3ftp)

CATH (3ftp)

Q3JQ67

Burkholderia pseudomallei (strain 1710b);

4nbv, download, 3D-view

SCOPe (4nbv)

CATH (4nbv)

B3R6T4

Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1);

Escherichia coli (strain K12);

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC);

3lyl, download, 3D-view

SCOPe (3lyl)

CATH (3lyl)

Q5NF68

Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4);

Helicobacter pylori (strain ATCC 700392 / 26695);

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);

3lls, download, 3D-view

SCOPe (3lls)

CATH (3lls)

A5TYW8

Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra);

4 entries

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv);

5 entries

Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh);

4m8s, download, 3D-view

SCOPe (4m8s)

CATH (4m8s)

A1KRY4

Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18);

Paraburkholderia xenovorans (strain LB400);

2c07, download, 3D-view

SCOPe (2c07)

CATH (2c07)

Q8I2S7

Plasmodium falciparum;

20 entries

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);

Rhizobium meliloti (strain 1021);

4kms, download, 3D-view

SCOPe (4kms)

CATH (4kms)

Q4UN54

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2);

3f9i, download, 3D-view

SCOPe (3f9i)

CATH (3f9i)

P50941

Rickettsia prowazekii (strain Madrid E);

Saccharomyces cerevisiae (strain ATCC 204508 / S288c);

3slk, download, 3D-view

SCOPe (3slk)

CATH (3slk)

Q9ALM5

Saccharopolyspora spinosa;

4zgw, download, 3D-view

SCOPe (4zgw)

CATH (4zgw)

V5YUZ7

Serratia marcescens;

3osu, download, 3D-view

SCOPe (3osu)

CATH (3osu)

P0A0H9

Staphylococcus aureus (strain Mu50 / ATCC 700699);

3sj7, download, 3D-view

SCOPe (3sj7)

CATH (3sj7)

Q2FZ53

Staphylococcus aureus (strain NCTC 8325);

2nm0, download, 3D-view

SCOPe (2nm0)

CATH (2nm0)

Q9S274

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145);

2z5l, download, 3D-view

SCOPe (2z5l)

CATH (2z5l)

O33954

Streptomyces fradiae;

Streptomyces nodosus;

3qp9, download, 3D-view

SCOPe (3qp9)

CATH (3qp9)

Q9ZGI4

Streptomyces venezuelae;

Synechococcus elongatus (strain PCC 7942);

1o5i, download, 3D-view

SCOPe (1o5i)

CATH (1o5i)

Q9X0Q1

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579);

3rkr, download, 3D-view

SCOPe (3rkr)

CATH (3rkr)

B2BKB1

uncultured bacterium Bio5;

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);

Yersinia pestis;

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24000

24200

4 * 24200, SDS-PAGE

25000

25500

27000

? * 27000, DNA sequence calculation

285679

28000

29000

2 * 29000, recombinant enzyme, SDS-PAGE; 4 * 29000, SDS-PAGE, tetrameric at higher enzyme concentrations

31000

4 * 31000, SDS-PAGE, wild-type and mutants

35000

Arabidopsis thaliana

sequence analysis, KCR2

700827

40000 - 46000

gel filtration, sucrose density gradient centrifugation

285678

44000

Euglena gracilis

gel filtration

285676

50400

recombinant enzyme, gel filtration

58400

recombinant enzyme, analytical ultracentrifugation

64000

gel filtration

97000

gel filtration

100000

Mycobacterium smegmatis

A0R723;

x * 100000, SDS-PAGE

740023

102000

gel filtration

110000

gel filtration, wild-type and mutants

116000

recombinant enzyme, analytical ultracentrifugation, tetrameric at higher enzyme concentrations

120000

gel filtration

130000

gel filtration

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dimer

2 * 29000, recombinant enzyme, SDS-PAGE

heterotetramer

homodimer

Saccharomyces cerevisiae

P35731;

x-ray crystallography

740558

homodimer or homotetramer

Vibrio cholerae

2 or 4 * 25000, SDS-PAGE

740630

homotetramer

4 * 31000, SDS-PAGE, wild-type and mutants

tetramer

10 entries

additional information

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additional information

the enzyme contains a signal peptide

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diffraction to 1.8 A, determinantion of initial phases by molecular replacement

Aquifex aeolicus VF5

684165

at 2.4 A resolution, space group P21 with unit-cell parameters a = 70.6, b = 120.7, c = 136.4 and beta = 104.4. The structure contains two tetramers displaying 222 symmetry (all chains are completely traced, although for some chains the electron density for residues 189-203 is poor) and 575 water molecules in the crystallographic asymmetric unit, but no bound cofactors or substrates

Bacillus anthracis

706640

diffraction to 2.4 A. Final model contains two tetramers displaying 222 symmetry and 575 water molecules in the crystallographic asymmetric unit, but no bound cofactors or substrates

structure determination and similarities within short-chain alcohol dehydrogenase family, catalytic mechanism

Brassica napus

285683

vapor diffusion method, using 0.1 M MES (pH 7.1), 1.6 M ammonium sulfate, and 10% (w/v) 1,4-dioxane

P14697;

sitting drop vapor diffusion method, using 15-18% (w/v) PEG3350 and 0.4 M ammonium acetate in sodium acetate buffer at pH 5.0

hanging drop vapor diffusion method, apoenzyme is crystallized by using 100 mM Na-HEPES (pH 7.5), 30% (w/v) PEG 400 and 200 mM magnesium chloride hexahydrate, while the complex with NADP+ is crystallized by using 200 mM sodium citrate tribasic dehydrate and 20% (w/v) PEG 3350

Mycobacterium smegmatis

A0R723;

740023

in complex with hexanoyl-CoA, hanging drop vapor diffusion method, using

740013

purified recombinant enzyme, vapour diffusion method, 10 mg/ml protein in 20 mM HEPES, pH 6.8, 0.5 M NaCl, 1 mM DTT, and 0.5 mM EDTA, is mixed with an equal volume of reservoir solution containing 0.1 M MES, pH 6.0, 35% v/v 2-methyl-2,4-pentanediol, and 0.2 M LiSO4, equilibration against 1 ml of mother liquor, room temperature, 6 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution

purified native and selenomethionine-labeled recombinant enzyme, hanging drop vapour diffusion method, 18°C, 2.5 mg/ml protein in 50 mm HEPES, pH 7.0, 5 mM Tris, pH 8.0, 0.15 mM ammonium sulfate, 6% PEG 4000, 0.1 M NaCl, 0.5 mM EDTA, and 0.5 mM DTT, mixed with reservoir solution containing 1 M HEPES, pH 7.0, 0.3 M ammonium sulfate, and 12% PEG 4000, plus 1 mM NADPH, X-ray diffraction structure determination and analysis at 2.3 A resolution

crystalized at a resolution of 2.25 A

Rickettsia prowazekii

P50941;

723843

apo-form complexed with NADPH, vapor diffusion method, using 10% (w/v) polyethylene glycol 4000, 10% (v/v) 2-propanol, 0.1 M HEPES pH 7.0 and 30% (v/v) jeffamine ED2001, 0.1 M HEPES pH 7.5

Saccharomyces cerevisiae

P35731;

740558

hanging drop vapor diffusion method, using 2.0-2.7 M ammonium sulfate and 0.1 M Tris (pH 9.0)

Vibrio cholerae

740630

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wild-type enzyme: about 15% loss of activity after 15 min, mutant enzyme E233K: about 60% loss of activity after 10 min,mutant enzyme A164T: about 20% loss of activity after 15 min, mutant enzyme A154T/E233K: about 80% loss of activity after 15 min

655871

wild-type enzyme: about 40% loss of activity after 15 min, mutant enzyme E233K: about 90% loss of activity after 10 min, mutant enzyme A164T: about 25% loss of activity after 15 min, mutant enzyme A154T/E233K: about 80% loss of activity after 5 min

655871

wild-type enzyme: about 70% loss of activity after 15 min, mutant enzyme E233K: complete loss of activity after 10 min, mutant enzyme A164T: about 60% loss of activity after 15 min, mutant enzyme A154T/E233K: complete loss of activity after 5 min

655871

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inactivation by dilution can be partly prevented by inclusion of NADPH