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(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
3-oxoacyl-[acyl-carrier protein] + NADPH + H+
beta-oxidation pathway
-
-
?
1,1,1-trifluoroacetone + NADPH
? + NADP+
792% relative activity compared to acetophenone
-
-
?
2',3',4',5',6'-pentafluoroacetophenone + NADPH
? + NADP+
3023% relative activity compared to acetophenone
-
-
?
2-octanone + NADPH
? + NADP+
15% relative activity compared to acetophenone
-
-
?
3'-fluoroacetophenone + NADPH
? + NADP+
100% relative activity compared to acetophenone
-
-
?
3-hydroxydecanoyl-N-acetylcysteamine + NADP+
3-oxodecanoyl-N-acetylcysteamine + NADPH + H+
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
3-oxoacyl-[acyl-carrier protein] + NADPH
3-hydroxyacyl-[acyl-carrier protein] + NADP+
3-oxoacyl-[acyl-carrier protein] + NADPH + H+
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxodecanoyl-CoA + NADPH
(R)-3-hydroxydecanoyl-CoA + NADP+
3-oxododecanoyl-CoA + NADPH
(R)-3-hydroxydodecanoyl-CoA + NADP+
3-oxohexanoyl-CoA + NADH + H+
(3R)-3-hydroxyhexanoyl-CoA + NAD+
3-oxohexanoyl-CoA + NADPH
(R)-3-hydroxyhexanoyl-CoA + NADP+
3-oxooctanoyl-CoA + NADPH
(R)-3-hydroxyoctanoyl-CoA + NADP+
4'-chloroacetophenone + NADPH
? + NADP+
115% relative activity compared to acetophenone
-
-
?
4'-fluoroacetophenone + NADPH
? + NADP+
62% relative activity compared to acetophenone
-
-
?
9,10-phenanthrene quinone + NADH + H+
?
-
-
-
?
9,10-phenanthrene quinone + NADPH + H+
?
low activity with NADPH
-
-
?
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
acetoacetyl-CoA + NADH + H+
?
-
-
-
?
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
acetoacetyl-CoA + NADPH
? + NADP+
-
wild-type shows less activity with acetoacetyl-CoA than with acetoacetyl-[acyl-carrier protein]
-
-
?
acetoacetyl-CoA + NADPH
D-3-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
acetoacetyl-CoA + NADPH + H+
3-hydroxybutyryl-CoA + NADP+
acetoacetyl-CoA + NADPH + H+
?
acetoacetyl-N-cysteamine + NADPH
D-3-hydroxybutyryl-N-cysteamine + NADP+
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
acetophenone + NADPH
? + NADP+
100% relative activity
-
-
?
alpha-chloroacetophenone + NADPH
? + NADP+
1885% relative activity compared to acetophenone
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
beta-ketobutyryl-CoA + NADPH
beta-hydroxybutyryl-CoA + NADP+
-
complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro
-
-
-
ethyl 4-chloroacetoacetate + NADPH
? + NADP+
29454% relative activity compared to acetophenone
-
-
?
ethyl acetoacetate + NADPH
? + NADP+
-
-
-
-
?
ethyl acetoacetate + NADPH
ethyl 3-hydroxybutyrate + NADP+
ethylbenzoylacetate + NADPH
? + NADP+
892% relative activity compared to acetophenone
-
-
?
methyl acetoacetate + NADPH
? + NADP+
-
is not a good substrate as ethyl acetoacetate
-
-
?
propiophenone + NADPH
? + NADP+
123% relative activity compared to acetophenone
-
-
?
propyl acetoacetate + NADPH
? + NADP+
-
as substrate it is limited to its weak solubility
-
-
?
additional information
?
-
3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
belongs to family of short-chain alcohol dehydrogenases (SDR) with catalytic triade Ser154, Tyr167 and Lys171, catalytic mechanism
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme is part of the dissociable fatty acid synthase type II multienzyme complex for de novo synthesis of fatty acids from acetyl-CoA and malonyl-CoA
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reduction step of fatty acid synthase
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme in fatty acid synthesis
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme in fatty acid synthesis
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
pH 6.0-7.0, equilibrium almost completely favors formation of the beta-hydroxyacyl ACP derivatives
product is the D-(-)-stereoisomer
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reduction step of fatty acid synthase
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reduction step of fatty acid synthase
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
-
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme in fatty acid synthesis
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH

3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH
3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH
3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding
-
-
r
3-oxodecanoyl-CoA + NADPH

(R)-3-hydroxydecanoyl-CoA + NADP+
-
-
-
-
?
3-oxodecanoyl-CoA + NADPH
(R)-3-hydroxydecanoyl-CoA + NADP+
-
-
-
?
3-oxododecanoyl-CoA + NADPH

(R)-3-hydroxydodecanoyl-CoA + NADP+
-
-
-
-
?
3-oxododecanoyl-CoA + NADPH
(R)-3-hydroxydodecanoyl-CoA + NADP+
-
-
-
?
3-oxohexanoyl-CoA + NADH + H+

(3R)-3-hydroxyhexanoyl-CoA + NAD+
-
-
-
-
?
3-oxohexanoyl-CoA + NADH + H+
(3R)-3-hydroxyhexanoyl-CoA + NAD+
-
-
-
?
3-oxohexanoyl-CoA + NADPH

(R)-3-hydroxyhexanoyl-CoA + NADP+
-
-
-
-
?
3-oxohexanoyl-CoA + NADPH
(R)-3-hydroxyhexanoyl-CoA + NADP+
-
-
-
?
3-oxooctanoyl-CoA + NADPH

(R)-3-hydroxyoctanoyl-CoA + NADP+
-
-
-
-
?
3-oxooctanoyl-CoA + NADPH
(R)-3-hydroxyoctanoyl-CoA + NADP+
-
-
-
?
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH

3-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
-
r
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
-
r
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH

D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
-
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
r
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
-
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH + H+

3-hydroxybutyryl-CoA + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH + H+
3-hydroxybutyryl-CoA + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH + H+
3-hydroxybutyryl-CoA + NADP+
-
-
-
?
acetoacetyl-CoA + NADPH + H+

?
low activity with NADPH
-
-
?
acetoacetyl-CoA + NADPH + H+
?
-
-
-
?
acetoacetyl-CoA + NADPH + H+
?
-
-
-
?
acetoacetyl-CoA + NADPH + H+
?
-
-
-
?
acetoacetyl-CoA + NADPH + H+
?
-
-
-
-
?
acetoacetyl-N-cysteamine + NADPH

D-3-hydroxybutyryl-N-cysteamine + NADP+
-
-
-
-
?
acetoacetyl-N-cysteamine + NADPH
D-3-hydroxybutyryl-N-cysteamine + NADP+
-
-
-
-
?
acetoacetyl-N-cysteamine + NADPH
D-3-hydroxybutyryl-N-cysteamine + NADP+
-
-
-
-
?
acetoacetyl-[acyl-carrier protein] + NADPH

D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
-
-
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
-
?
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
much greater specificity for acetoacetyl-ACP than for either of the model substrates acetoacetyl-CoA or acetoacetyl-N-acetylcysteamine
-
-
?
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
-
-
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
-
?
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
r
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
r
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
-
-
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
much greater specificity for acetoacetyl-ACP than for either of the model substrates acetoacetyl-CoA or acetoacetyl-N-acetylcysteamine
-
-
?
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
Arg187 and Arg230 are critical residues for the FabG-acyl-carrier protein interactions, significance of the positively charged/hydrophobic patch located adjacent to the active site cavities of FabG for interactions with acyl carrier protein
-
-
?
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
-
-
acetoacetyl-[acyl-carrier protein] + NADPH
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
-
-
r
beta-ketoacyl-[acyl-carrier protein] + NADPH

beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reductive step in the elongation cycle of fatty acid biosynthesis
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reductive step in the elongation cycle of fatty acid biosynthesis
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+

beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
?
ethyl acetoacetate + NADPH

ethyl 3-hydroxybutyrate + NADP+
-
-
-
-
?
ethyl acetoacetate + NADPH
ethyl 3-hydroxybutyrate + NADP+
-
-
-
-
?
additional information

?
-
-
animals, yeast and Mycobacteria have fatty acid synthetase containing all the individual activities on one or two multifunctional polypeptide chains, plants and most bacterial systems, including E. coli, possess individual monofunctional enzymes and a separate acyl carrier protein
-
-
-
additional information
?
-
-
only D-(-) isomer is oxidized
-
-
-
additional information
?
-
-
beta-ketoacyl thioesters of CoA and pantetheine metabolized at slower rates
-
-
-
additional information
?
-
-
equally active on beta-ketoacyl-ACP derivatives of C4 to C16
-
-
-
additional information
?
-
-
broad specificity for chain length of substrates
-
-
-
additional information
?
-
-
broad specificity for chain length of substrates
-
-
-
additional information
?
-
-
L-isomer completely inactive
-
-
-
additional information
?
-
-
L-isomer completely inactive
-
-
-
additional information
?
-
-
catalyzes also the reduction of the beta-keto group of unsaturated acyl chains during biosynthesis of unsaturated fatty acids
-
-
-
additional information
?
-
-
FabG substrate specificity overview
-
-
-
additional information
?
-
-
FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis
-
-
-
additional information
?
-
no activity with NADH
-
-
-
additional information
?
-
no activity with NADH
-
-
-
additional information
?
-
FabG1 is able to act on shorter (C4) acyl substrates
-
-
-
additional information
?
-
FabG1 is able to act on shorter (C4) acyl substrates
-
-
-
additional information
?
-
FabG1 is able to act on shorter (C4) acyl substrates
-
-
-
additional information
?
-
-
animals, yeast and Mycobacteria have fatty acid synthetase containing all the individual activities on one or two multifunctional polypeptide chains, plants and most bacterial systems, including E. coli, possess individual monofunctional enzymes and a separate acyl carrier protein
-
-
-
additional information
?
-
-
the beta-ketoacyl acyl carrier protein reductase activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths
-
-
-
additional information
?
-
-
the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa
-
-
-
additional information
?
-
fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA
-
-
-
additional information
?
-
-
fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA
-
-
-
additional information
?
-
FabG substrate specificity overview
-
-
-
additional information
?
-
-
L-isomer completely inactive
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
3-oxoacyl-[acyl-carrier protein] + NADPH + H+
A5HIF6
beta-oxidation pathway
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
3-oxoacyl-[acyl-carrier protein] + NADPH
3-hydroxyacyl-[acyl-carrier protein] + NADP+
3-oxoacyl-[acyl-carrier protein] + NADPH + H+
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
acetoacetyl-CoA + NADPH
3-hydroxybutyryl-CoA + NADP+
-
-
-
-
?
acetoacetyl-CoA + NADPH + H+
3-hydroxybutyryl-CoA + NADP+
beta-ketoacyl-[acyl-carrier protein] + NADPH
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
additional information
?
-
3-oxoacyl-[acyl-carrier protein] + NADPH

(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme is part of the dissociable fatty acid synthase type II multienzyme complex for de novo synthesis of fatty acids from acetyl-CoA and malonyl-CoA
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reduction step of fatty acid synthase
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme in fatty acid synthesis
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme in fatty acid synthesis
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reduction step of fatty acid synthase
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reduction step of fatty acid synthase
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
enzyme in fatty acid synthesis
-
-
?
3-oxoacyl-[acyl-carrier protein] + NADPH

3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
r
3-oxoacyl-[acyl-carrier protein] + NADPH
3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
r
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
-
-
-
?
a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
P35731
-
-
-
?
acetoacetyl-CoA + NADPH + H+

3-hydroxybutyryl-CoA + NADP+
P14697
-
-
-
?
acetoacetyl-CoA + NADPH + H+
3-hydroxybutyryl-CoA + NADP+
P14697
-
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH

beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reductive step in the elongation cycle of fatty acid biosynthesis
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
first reductive step in the elongation cycle of fatty acid biosynthesis
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+

beta-hydroxyacyl-[acyl-carrier protein] + NADP+
A0R723
-
-
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
beta-hydroxyacyl-[acyl-carrier protein] + NADP+
A0R723
-
-
-
?
additional information

?
-
-
catalyzes also the reduction of the beta-keto group of unsaturated acyl chains during biosynthesis of unsaturated fatty acids
-
-
-
additional information
?
-
-
FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis
-
-
-
additional information
?
-
-
the beta-ketoacyl acyl carrier protein reductase activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths
-
-
-
additional information
?
-
-
the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa
-
-
-
additional information
?
-
A5HIF6
fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA
-
-
-
additional information
?
-
-
fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA
-
-
-
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(E)-1-(4-methylpiperidin-1-yl)-3-phenylprop-2-en-1-one
80% residual activity
(E)-2-nitrophenyl cinnamate
-
(E)-3-phenoxybenzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate
61% residual activity
(E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate
-
(E)-benzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate
71% residual activity
(E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate
69% residual activity
1,2,3,4,6-penta-O-galloyl-beta-D-glucose
4-acetoxyanthecotulide
-
most active linear sesquiterpene lactone from Anthemis auriculata against FabG
4-hydroxyanthecotulide
-
linear sesquiterpene lactone from Anthemis auriculata
Acyl carrier protein
-
beta-ketobutyryl-CoA reductase activity of FabG1 is inhibited, activity of FabG2 is unaffected
acyl-carrier protein
-
inhibition of wild-type with increasing concentrations
anthecotulide
-
linear sesquiterpene lactone from Anthemis auriculata
Bithionol
over 75% inhibition at 0.02 mM, IC50: 0.010 mM
bromochlorophen
an anthelmintic agent, over 75% inhibition at 0.02 mM, IC50: 0.0154 mM
chlorogenic acid
-
IC50: 0.881-0.948 mM
di-resorcinol sulfide
over 75% inhibition at 0.02 mM, IC50: 0.0038 mM
DL-3-hydroxybutyryl-CoA
-
competitive product inhibition
epigallocatechin gallate
-
-
grape skin extract
-
inhibits the overall reaction and beta-ketoacyl reductase reaction of FAS with IC50 values of 4.61 microg/ml and 20.3 microg/ml, respectively. Inhibits the overall reaction of FAS competitively with acetyl-CoA, noncompetitively with malonyl-CoA and in a mixed manner with NADPH
-
Guanidinium chloride
-
FabG is fully unfolded at 4 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. In presence of NADPH, there is no stabilization of FabG in case of equilibrium unfolding with guanidinium chloride
Hexachlorophene
an anthelmintic and antimicrobial agent, over 75% inhibition at 0.02 mM, IC50: 0.002 mM
N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide
69% residual activity
p-chloromercuribenzoate
-
-
resveratrol
-
inhibits the overall reaction and beta-ketoacyl reductase reaction of FAS with IC50 values of 11.1 microg/ml and 21.9 microg/ml, respectively. In 3 T3-L1 preadipocytes, resveratrol reduces lipid accumulation remarkably
RNAi
-
suppresses KCR activity, which results in a reduction of cuticular wax load and affects very-long-chain fatty acid composition of sphingolipids, seed triacylglycerols, and root glycerolipids. RNAi-suppressed Arabidopsis KCR1 plants display fused vegetative and reproductive organs, an abnormal root morphology and have abnormal trichome and epidermal cell morphology
-
Urea
-
FabG is fully unfolded at 6 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. Two states in the reversible unfolding process of FabG in presence of NADPH, one is an activity-enhanced state and the other, an inactive state in case of equilibrium unfolding with urea
1,2,3,4,6-penta-O-galloyl-beta-D-glucose

-
mixed type of inhibition. IC50 value 0.9 microgramm per ml, Ki value 0.21 microgramm per ml
1,2,3,4,6-penta-O-galloyl-beta-D-glucose
-
compound is transported across cancer cell membrane to further down-regulate FAS and activate caspase-3 in MDA-MB-231 cells. Compared with other FAS inhibitors, including catechin gallate and morin, 1,2,3,4,6-penta-O-galloyl-beta-D-glucose involves a higher reversible fast-binding inhibition with an irreversible slow-binding inhibition, i.e. saturation kinetics with a dissociation constant of 0.59 microM and a limiting rate constant of 0.16 per min. The major reacting site of PGG is on the beta-ketoacyl reduction domain of FAS. Compound exhibits different types of inhibitions against the three substrates in the FAS overall reaction
1,2,3,4,6-penta-O-galloyl-beta-D-glucose
-
minimal inhibitory concentration 0.125 mg/ml
1,2,3,4,6-penta-O-galloyl-beta-D-glucose
-
minimal inhibitory concentration 0.25 mg/ml
3-hydroxybutyryl-CoA

-
noncompetitive product inhibition pattern
3-hydroxybutyryl-CoA
competitive product inhibition
NADP+

-
competitive product inhibition
NADP+
-
noncompetitive product inhibition pattern
NADP+
-
competitive product inhibition
Phenylglyoxal

-
-
Phenylglyoxal
-
with NADPH 45% remaining activity, without total loss of activity
quercetin

-
-
Tannic acid

-
-
Tannic acid
-
; strongest inhibition on FabG, shows time-dependent irreversible inhibition
Tannic acid
-
displays very strong inhibition
Tannic acid
-
displays very strong inhibition
additional information

-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
cinnamic acid derivatives can be accommodated in the substrate-binding region of the active site, above the nicotinamide moiety of the NADPH cofactor
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge; inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Leaf extracts of Acer saccharum and Acer truncatum Bunge display time-dependent irreversible inhibition of FabG, whereas leaf extracts of Acer platanoides, Acer campestre and Acer rubrum show reversible inhibition
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
no inhibition by triclosan
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Is inhibited by the leaf extracts from all five kinds of maples more effectively than are other Gram-negative bacteria strains
-
additional information
-
FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is above 1.28 mM
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Is inhibited by the leaf extracts from all five kinds of maples more effectively than are other Gram-negative bacteria strains
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge. Is inhibited by the leaf extracts from all five kinds of maples more effectively than are other Gram-negative bacteria strains
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
not: N-ethylmaleimide, arsenite
-
additional information
-
FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.32-0.64 mM
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge
-
additional information
-
inhibition by leaf extracts from Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge, except strain 04-5
-
additional information
-
FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.01-0.02 mM
-
additional information
-
FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.025-0.05 mM
-
additional information
-
FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum), strongest inhibition with 40% ethanol extract of galangal. Inhibition is consisted of both reversible and irreversible inhibition. Inhibits FabG in a competitive pattern against NADPH, irreversible inhibition presents two phases (slow and fast one)
-
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89.2
1,1,1-trifluoroacetone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
5.72
2',3',4',5',6'-pentafluoroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
7.38
3'-fluoroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
14.34
4'-chloroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
12.29
4'-fluoroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.0057 - 2.2
acetoacetyl-CoA
48
acetoacetyl-N-acetylcysteamine
-
-
0.003 - 0.017
acetoacetyl-[acyl-carrier protein]
46.92
acetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
2.71
alpha-chloroacetophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.29 - 4.06
beta-ketobutyryl-CoA
8.31
ethyl 4-chloroacetoacetate
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.127
ethyl acetoacetate
-
-
1.92
ethylbenzoylacetate
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.035
N-(2-sulfanylethyl)acetamide
-
-
0.29
NADP+
with 3-hydroxydecanoyl-N-acetylcysteamine as cosubstrate, pH and temperature not specified in the publication
6.03
propiophenone
with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
additional information
additional information
-
0.0057
acetoacetyl-CoA

wild type enzyme, at pH 8.0 and 30°C
0.0136
acetoacetyl-CoA
mutant enzyme Q47L, at pH 8.0 and 30°C
0.0159
acetoacetyl-CoA
mutant enzyme Q47L, at pH 8.0 and 30°C
0.075
acetoacetyl-CoA
pH 7.5
0.165
acetoacetyl-CoA
-
pH 7.6, 30°C
0.261
acetoacetyl-CoA
-
-
0.43
acetoacetyl-CoA
-
pH 6.8, 22°C, recombinant enzyme
0.43
acetoacetyl-CoA
-
wild-type
0.44
acetoacetyl-CoA
-
mutant R230A
0.45
acetoacetyl-CoA
-
mutant R187A
0.47
acetoacetyl-CoA
-
mutant R187A/R230A; mutant R187E
0.49
acetoacetyl-CoA
-
mutant R230E
0.51
acetoacetyl-CoA
pH 6.8, 25°C, recombinant enzyme
0.58
acetoacetyl-CoA
-
mutant R187E/R230E
0.8158
acetoacetyl-CoA
wild type enzyme, at pH 8.0 and 25°C
0.8871
acetoacetyl-CoA
mutant enzyme S36A, at pH 8.0 and 25°C
0.9913
acetoacetyl-CoA
mutant enzyme S40A, at pH 8.0 and 25°C
1
acetoacetyl-CoA
pH and temperature not specified in the publication
2.2
acetoacetyl-CoA
-
pH 7.6, 30°C
0.003
acetoacetyl-[acyl-carrier protein]

-
-
0.0037
acetoacetyl-[acyl-carrier protein]
-
-
0.0079
acetoacetyl-[acyl-carrier protein]
-
-
0.009
acetoacetyl-[acyl-carrier protein]
-
-
0.017
acetoacetyl-[acyl-carrier protein]
-
-
0.29
beta-ketobutyryl-CoA

-
pH 7.0, 30°C, FabG1
4.06
beta-ketobutyryl-CoA
-
pH 7.0, 30°C, FabG2
0.0093
NADPH

-
-
0.0205
NADPH
wild type enzyme, at pH 8.0 and 25°C
0.023
NADPH
-
+ acetoacetyl-[acyl-carrier-protein]
0.026
NADPH
-
pH 7.6, 30°C
0.0264
NADPH
mutant enzyme S36A, at pH 8.0 and 25°C
0.0412
NADPH
-
in the presence of 1.5 M urea, pH 7.0, 25°C
0.043
NADPH
-
pH 6.8, 22°C, recombinant enzyme
0.0434
NADPH
-
in the presence of 1 M urea, pH 7.0, 25°C
0.0461
NADPH
-
without urea, pH 7.0, 25°C
0.0475
NADPH
pH 6.8, 25°C, recombinant enzyme
0.0571
NADPH
mutant enzyme S40A, at pH 8.0 and 25°C
0.149
NADPH
wild type enzyme, at pH 8.0 and 30°C
0.289
NADPH
mutant enzyme Q47L, at pH 8.0 and 30°C
0.29
NADPH
with acetoacetyl-CoA as cosubstrate, pH and temperature not specified in the publication
0.4553
NADPH
-
in the presence of 4 M urea, pH 7.0, 25°C
0.617
NADPH
mutant enzyme Q47L, at pH 8.0 and 30°C
additional information
additional information

-
-
-
additional information
additional information
-
-
-
additional information
additional information
kinetic analysis of the recombinant enzyme, kinetic mechanism
-
additional information
additional information
-
detailed steady-state kinetic analysis, solvent and multiple kinetic isotope effects, random bi bi kinetic mechanism
-
additional information
additional information
-
detailed kinetic and thermodynamic analysis, solvent and multiple kinetic isotope effects, kinetic mechanism
-
additional information
additional information
-
cooperativity kinetics, binding constants of substrates and cofactors
-
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0.0017 - 0.0018
-
recombinant refolded enzyme
0.0019
-
recombinant soluble enzyme
0.02
with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.06
mutant enzyme K173A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
0.08
with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.13
with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0; with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.15
with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.16
with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.44
mutant enzyme R34A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C; mutant enzyme R34A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
0.46
mutant enzyme K152A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
0.5
mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
0.65
mutant enzyme Y169A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
0.85
mutant enzyme Y169A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
1.03
with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
1.16
with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
1.2
mutant enzyme K173A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
1.3
mutant enzyme K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
1.6
mutant enzyme R168E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
1.7
mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
2.4
mutant enzyme K173A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
2.45
with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
2.6
wild type enzyme, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
3
-
mutant R187A/R230A, acyl-carrier protein-dependent spectroscopic assay
3.1
mutant enzyme Y169A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
3.5
wild type enzyme, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
3.93
with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
4
-
mutant R230E, acyl-carrier protein-dependent spectroscopic assay
4.3
mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
4.5
mutant enzyme K173A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
5.4
mutant enzyme D42A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C
5.5
mutant enzyme K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
6.1
-
mutant R230A, acyl-carrier protein-dependent spectroscopic assay
6.9
-
mutant R187A, acyl-carrier protein-dependent spectroscopic assay
7.3
mutant enzyme D42A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
7.9
mutant enzyme R168E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
8.4
mutant enzyme Q126E/R168E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
14.8
mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
15.7
mutant enzyme K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
17.4
mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
19.3
mutant enzyme K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
20
mutant enzyme D42A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
20.5
wild type enzyme, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
20.7
mutant enzyme R34A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
20.8
wild type enzyme, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
21
mutant enzyme R34A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
21.7
mutant enzyme R168E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
22
mutant enzyme K152A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
25.5
mutant enzyme K152A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
31.8
mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C
38.29
with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
42.6
-
mutant R187E/R230E, acyl-carrier protein-independent spectroscopic assay
45.6
-
mutant R187A/R230A, acyl-carrier protein-independent spectroscopic assay
54.2
-
mutant R230E, acyl-carrier protein-independent spectroscopic assay
54.5
-
mutant R187E, acyl-carrier protein-independent spectroscopic assay
55.3
-
mutant R230A, acyl-carrier protein-independent spectroscopic assay
57.9
-
mutant R187A, acyl-carrier protein-independent spectroscopic assay
70.6
-
wild-type, acyl-carrier protein-dependent spectroscopic assay
74.6
mutant enzyme D42A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C
280
-
acetoacetyl-N-acetylcysteamine
3839
-
recombinant protein, cosubstrate NADH, pH 7.0, phosphate buffer
9401
-
purified enzyme, protein determination by amino acid analysis
83140
-
cosubstrate NADPH, pH 8.35, carbonate buffer
136000
-
purified enzyme, protein determination by calculation from absorption at 280 nm
180400
-
recombinant protein, cosubstrate NADPH, pH 7.0, phosphate buffer
2

mutant enzyme Y169A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
2
-
mutant R187E/R230E, acyl-carrier protein-dependent spectroscopic assay
6.3

mutant enzyme R168E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C
6.3
-
mutant R187E, acyl-carrier protein-dependent spectroscopic assay
59.8

-
purified recombinant enzyme
59.8
-
wild-type, acyl-carrier protein-independent spectroscopic assay
additional information

the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639.
additional information
-
the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639.
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A9NFJ2
Acholeplasma laidlawii (strain PG-8A);
Q9Y8Y1
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1);
Aquifex aeolicus (strain VF5);
A0A1S0QWK0
Bacillus anthracis;
Bacillus subtilis (strain 168);
Brucella abortus (strain 2308);
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094);
B0CL43
Brucella suis (strain ATCC 23445 / NCTC 10510);
Burkholderia multivorans (strain ATCC 17616 / 249);
Q3JQ67
Burkholderia pseudomallei (strain 1710b);
B3R6T4
Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1);
Escherichia coli (strain K12);
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC);
Q5NF68
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4);
Helicobacter pylori (strain ATCC 700392 / 26695);
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
A5TYW8
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra);
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv);
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh);
A1KRY4
Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18);
Paraburkholderia xenovorans (strain LB400);
Q8I2S7
Plasmodium falciparum;
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
Rhizobium meliloti (strain 1021);
Q4UN54
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2);
P50941
Rickettsia prowazekii (strain Madrid E);
Saccharomyces cerevisiae (strain ATCC 204508 / S288c);
Q9ALM5
Saccharopolyspora spinosa;
V5YUZ7
Serratia marcescens;
P0A0H9
Staphylococcus aureus (strain Mu50 / ATCC 700699);
Q2FZ53
Staphylococcus aureus (strain NCTC 8325);
Q9S274
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145);
O33954
Streptomyces fradiae;
Q9ZGI4
Streptomyces venezuelae;
Synechococcus elongatus (strain PCC 7942);
Q9X0Q1
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099);
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579);
B2BKB1
uncultured bacterium Bio5;
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);
O67610
Aquifex aeolicus (strain VF5);

O67610
Aquifex aeolicus (strain VF5);
P40806
Bacillus subtilis (strain 168);

P40806
Bacillus subtilis (strain 168);
Q2YJS1
Brucella abortus (strain 2308);

Q2YL80
Brucella abortus (strain 2308);
Q8YJQ6
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094);

Q8YJQ6
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094);
A0A0H3KNE7
Burkholderia multivorans (strain ATCC 17616 / 249);

A0A0H3KNE7
Burkholderia multivorans (strain ATCC 17616 / 249);
A0A0H3KNE7
Burkholderia multivorans (strain ATCC 17616 / 249);
P0AEK2
Escherichia coli (strain K12);

P0AEK2
Escherichia coli (strain K12);
P0AEK2
Escherichia coli (strain K12);
A0A0H2V833
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC);

A0A0H2V833
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC);
O25286
Helicobacter pylori (strain ATCC 700392 / 26695);

O25286
Helicobacter pylori (strain ATCC 700392 / 26695);
A0R4P1
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);

P71534
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
P71534
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
P71534
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
A0QPE7
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
A0QP46
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
A0QP46
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155);
P9WGT3
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv);

P9WGT3
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv);
P9WGT3
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv);
P9WGT3
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv);
O53665
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh);

O53665
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh);
O53665
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh);
O53665
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh);
O53665
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh);
Q13GL4
Paraburkholderia xenovorans (strain LB400);

Q13HF0
Paraburkholderia xenovorans (strain LB400);
Q9RPT1
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);

O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
O54438
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1);
Q92W70
Rhizobium meliloti (strain 1021);

Q92W71
Rhizobium meliloti (strain 1021);
Q7ANT0
Rhizobium meliloti (strain 1021);
Q92WA6
Rhizobium meliloti (strain 1021);
Q92U42
Rhizobium meliloti (strain 1021);
Q92L02
Rhizobium meliloti (strain 1021);
Q92TX5
Rhizobium meliloti (strain 1021);
P35731
Saccharomyces cerevisiae (strain ATCC 204508 / S288c);

P35731
Saccharomyces cerevisiae (strain ATCC 204508 / S288c);
P35731
Saccharomyces cerevisiae (strain ATCC 204508 / S288c);
Q93NW7
Streptomyces nodosus;

Q93NW7
Streptomyces nodosus;
Q93NW7
Streptomyces nodosus;
Q93NW7
Streptomyces nodosus;
Q93NW7
Streptomyces nodosus;
Q93NW7
Streptomyces nodosus;
Q93NX9
Streptomyces nodosus;
Q31QF3
Synechococcus elongatus (strain PCC 7942);

Q31QF3
Synechococcus elongatus (strain PCC 7942);
Q5SK98
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579);

Q5SL78
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579);
Q9KQH7
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);

Q9KQH7
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);
Q9KQH7
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);
Q9KQH7
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);
Q9KQH7
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);
Q9KQH7
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);
A0A0H2W5K6
Yersinia pestis;

A0A0H2W5K6
Yersinia pestis;
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