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Literature summary for 1.1.1.100 extracted from

  • Karmodiya, K.; Surolia, N.
    A unique and differential effect of denaturants on cofactor mediated activation of Plasmodium falciparum beta-ketoacyl-ACP reductase (2008), Proteins Struct. Funct. Genet., 70, 528-538.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information affinity of acyl-carrier protein for FabG is increased by 3fold in the presence of 1 M urea Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
into pET-28a vector and expressed in Escherichia coli BL21 (DE3) codon plus Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
Guanidinium chloride FabG is fully unfolded at 4 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. In presence of NADPH, there is no stabilization of FabG in case of equilibrium unfolding with guanidinium chloride Plasmodium falciparum
Urea FabG is fully unfolded at 6 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. Two states in the reversible unfolding process of FabG in presence of NADPH, one is an activity-enhanced state and the other, an inactive state in case of equilibrium unfolding with urea Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0412
-
NADPH in the presence of 1.5 M urea, pH 7.0, 25°C Plasmodium falciparum
0.0434
-
NADPH in the presence of 1 M urea, pH 7.0, 25°C Plasmodium falciparum
0.0461
-
NADPH without urea, pH 7.0, 25°C Plasmodium falciparum
0.4553
-
NADPH in the presence of 4 M urea, pH 7.0, 25°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoacyl-[acyl-carrier protein] + NADPH
-
Plasmodium falciparum (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
acetoacetyl-CoA + NADPH
-
Plasmodium falciparum 3-hydroxybutyryl-CoA + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer cross-linking of native and up to 1 M urea-treated FabG Plasmodium falciparum

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP reductase
-
Plasmodium falciparum
FabG
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
148.6
-
NADPH in the presence of 4 M urea, pH 7.0, 25°C Plasmodium falciparum
187.3
-
NADPH in the presence of 1.5 M urea, pH 7.0, 25°C Plasmodium falciparum
250
-
NADPH without urea, pH 7.0, 25°C Plasmodium falciparum
344.7
-
NADPH in the presence of 1 M urea, pH 7.0, 25°C Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Plasmodium falciparum