Application | Comment | Organism |
---|---|---|
drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Staphylococcus aureus |
drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Pseudomonas aeruginosa |
drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Streptococcus pneumoniae |
drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Streptococcus sp. |
drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | synthetic construct |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL21 (DE3) | synthetic construct |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
kaempferol | - |
synthetic construct | |
additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is above 1.28 mM | Pseudomonas aeruginosa | |
additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.32-0.64 mM | Staphylococcus aureus | |
additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.01-0.02 mM | Streptococcus pneumoniae | |
additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.025-0.05 mM | Streptococcus sp. | |
additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum), strongest inhibition with 40% ethanol extract of galangal. Inhibition is consisted of both reversible and irreversible inhibition. Inhibits FabG in a competitive pattern against NADPH, irreversible inhibition presents two phases (slow and fast one) | synthetic construct | |
quercetin | - |
synthetic construct |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
ATCC27853 | - |
Pseudomonas aeruginosa ATCC 27853 | - |
ATCC27853 | - |
Staphylococcus aureus | - |
ATCC25923 | - |
Staphylococcus aureus ATCC 25923 | - |
ATCC25923 | - |
Streptococcus pneumoniae | - |
- |
- |
Streptococcus sp. | - |
alpha-haemolytic strain 32213-7 and beta-haemolytic strain 32210-20 | - |
synthetic construct | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by nickel chelation affinity chromatography | synthetic construct |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-80°C, 50% glycerol | synthetic construct |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl acetoacetate + NADPH | - |
synthetic construct | ethyl 3-hydroxybutyrate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP reductase | - |
Staphylococcus aureus |
beta-ketoacyl-ACP reductase | - |
Pseudomonas aeruginosa |
beta-ketoacyl-ACP reductase | - |
Streptococcus pneumoniae |
beta-ketoacyl-ACP reductase | - |
Streptococcus sp. |
beta-ketoacyl-ACP reductase | - |
synthetic construct |
FabG | - |
Staphylococcus aureus |
FabG | - |
Pseudomonas aeruginosa |
FabG | - |
Streptococcus pneumoniae |
FabG | - |
Streptococcus sp. |
FabG | - |
synthetic construct |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay conditions | synthetic construct |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
synthetic construct |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC(50) value of 4.47 microg/ml for extract of galangal, pH 7.0 | synthetic construct | additional information | |
0.0212 | - |
pH 7.0 | synthetic construct | kaempferol | |
0.3048 | - |
pH 7.0 | synthetic construct | quercetin |