2.4.1.370: inositol phosphorylceramide mannosyltransferase
This is an abbreviated version!
For detailed information about inositol phosphorylceramide mannosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.370
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2.4.1.370
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mannosylation
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sphingolipids
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ca2+-sensitive
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ipc
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albicans
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multicopy
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ca2+-induced
- 2.4.1.370
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mannosylation
- sphingolipids
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ca2+-sensitive
- ipc
- albicans
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multicopy
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ca2+-induced
Reaction
Synonyms
BCL21, Csg1, Csg1p, CSH1, Csh1p, SUR1, YBR161w
ECTree
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Engineering
Engineering on EC 2.4.1.370 - inositol phosphorylceramide mannosyltransferase
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N247Q
site-directed mutagenesis, N-glycosylation of the mutant is reduced compared to the wild-type enzyme
N51Q
site-directed mutagenesis, N-glycosylation of the mutant is reduced compared to the wild-type enzyme
N51Q/N247Q
site-directed mutagenesis, N-glycosylation of the mutant is reduced compared to the wild-type enzyme
N247Q
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site-directed mutagenesis, N-glycosylation of the mutant is reduced compared to the wild-type enzyme
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N51Q
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site-directed mutagenesis, N-glycosylation of the mutant is reduced compared to the wild-type enzyme
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N51Q/N247Q
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site-directed mutagenesis, N-glycosylation of the mutant is reduced compared to the wild-type enzyme
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additional information
association between recombinant HA3-tagged Csg2p and His6-myc-tagged Csg1p. The N-terminally truncated Csg2p retains the Csg1p-interacting site. The small amounts of M(IP)2C-B'/B and MIPC-C observed in the DELTAcsg2 cells are completely lacking after the introduction of the DELTAcsg1 mutation. The DELTAcsg2 mutant, as well as the DELTAcsg1/DELTAcsh1 double mutant, is highly sensitive to Ca2+ and does not grow on the YPD plate medium. Overexpression of Csg1p suppresses the Ca2+-sensitive phenotype of a csg2 mutant, but in the inverse experiment, overexpression of Csg2p does not suppress the Ca2+-sensitivity of a csg1 mutant
additional information
association between recombinant HA3-tagged Csg2p and His6-myc-tagged Csg1p. The N-terminally truncated Csg2p retains the Csg1p-interacting site. The small amounts of M(IP)2C-B'/B and MIPC-C observed in the DELTAcsg2 cells are completely lacking after the introduction of the DELTAcsg1 mutation. The DELTAcsg2 mutant, as well as the DELTAcsg1/DELTAcsh1 double mutant, is highly sensitive to Ca2+ and does not grow on the YPD plate medium. Overexpression of Csg1p suppresses the Ca2+-sensitive phenotype of a csg2 mutant, but in the inverse experiment, overexpression of Csg2p does not suppress the Ca2+-sensitivity of a csg1 mutant
additional information
generation of csg1 and csg2 mutants, phenotypes, overview. The csg1DELTA strain (689DELTAcsg1) is constructed by disrupting CSG1 in DBY689 (MATa ura3-52 leu2). The isogenic csg2D (689Dcsg2) and ccc2D (689Dccc2) strains are constructed by transforming DBY689 with the CSG2- or CCC2-disrupting constructs. Cu2+ reverses the Ca2+ sensitivity of csg1 mutant cells. Kinetics of Ca2+-induced cell death, in both csg1 and csg2 mutants, Ca2+ accumulation begins after a delay of about 30 min following addition of 10 mM Ca2+. This overaccumulation phenotype is specific for Ca2+, since Sr2+ accumulation in the csg1 and csg2 mutant cells is comparable to Sr2+ accumulation by wild-type cells. Calcineurin, calmodulin and phospholipase C do not mediate the Ca2+-induced death of the csg mutants
additional information
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generation of csg1 and csg2 mutants, phenotypes, overview. The csg1DELTA strain (689DELTAcsg1) is constructed by disrupting CSG1 in DBY689 (MATa ura3-52 leu2). The isogenic csg2D (689Dcsg2) and ccc2D (689Dccc2) strains are constructed by transforming DBY689 with the CSG2- or CCC2-disrupting constructs. Cu2+ reverses the Ca2+ sensitivity of csg1 mutant cells. Kinetics of Ca2+-induced cell death, in both csg1 and csg2 mutants, Ca2+ accumulation begins after a delay of about 30 min following addition of 10 mM Ca2+. This overaccumulation phenotype is specific for Ca2+, since Sr2+ accumulation in the csg1 and csg2 mutant cells is comparable to Sr2+ accumulation by wild-type cells. Calcineurin, calmodulin and phospholipase C do not mediate the Ca2+-induced death of the csg mutants
additional information
the DELTAcsg1/DELTAcsh1 double mutant is highly sensitive to Ca2+ and does not grow on the YPD plate medium
additional information
the DELTAcsg1/DELTAcsh1 double mutant is highly sensitive to Ca2+ and does not grow on the YPD plate medium
additional information
-
association between recombinant HA3-tagged Csg2p and His6-myc-tagged Csg1p. The N-terminally truncated Csg2p retains the Csg1p-interacting site. The small amounts of M(IP)2C-B'/B and MIPC-C observed in the DELTAcsg2 cells are completely lacking after the introduction of the DELTAcsg1 mutation. The DELTAcsg2 mutant, as well as the DELTAcsg1/DELTAcsh1 double mutant, is highly sensitive to Ca2+ and does not grow on the YPD plate medium. Overexpression of Csg1p suppresses the Ca2+-sensitive phenotype of a csg2 mutant, but in the inverse experiment, overexpression of Csg2p does not suppress the Ca2+-sensitivity of a csg1 mutant
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additional information
-
generation of csg1 and csg2 mutants, phenotypes, overview. The csg1DELTA strain (689DELTAcsg1) is constructed by disrupting CSG1 in DBY689 (MATa ura3-52 leu2). The isogenic csg2D (689Dcsg2) and ccc2D (689Dccc2) strains are constructed by transforming DBY689 with the CSG2- or CCC2-disrupting constructs. Cu2+ reverses the Ca2+ sensitivity of csg1 mutant cells. Kinetics of Ca2+-induced cell death, in both csg1 and csg2 mutants, Ca2+ accumulation begins after a delay of about 30 min following addition of 10 mM Ca2+. This overaccumulation phenotype is specific for Ca2+, since Sr2+ accumulation in the csg1 and csg2 mutant cells is comparable to Sr2+ accumulation by wild-type cells. Calcineurin, calmodulin and phospholipase C do not mediate the Ca2+-induced death of the csg mutants
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additional information
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the DELTAcsg1/DELTAcsh1 double mutant is highly sensitive to Ca2+ and does not grow on the YPD plate medium
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