Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.1.54: formate C-acetyltransferase

This is an abbreviated version!
For detailed information about formate C-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.54

Reaction

acetyl-CoA
+
formate
=
CoA
 +
pyruvate

Synonyms

AF_1449, formate acetyltransferase, PFL, PFL I, Pfl1, PflB, phosphotransferase system enzyme I, pyruvate formate lyase, pyruvate formate-lyase, pyruvate-formate lyase, pyruvate:formate lyase, pyruvic formate-lyase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.54 formate C-acetyltransferase

Cloned

Cloned on EC 2.3.1.54 - formate C-acetyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli SP 264 contains plasmid p29with pfl gene, identification of pfl gene product as a 80kDa polypeptide
-
expressed in Escherichia coli BL21(DE3), pET9a expression vector, transformation into pyruvate formate-lyase-deficient Escherichia coli strain BL21, capacity for formate excretion shown by in vitro tests
expressed in Escherichia coli DC10B cells
-
gene deletion is transduced via Bacteriophage P1
-
gene pfl from strain JM109, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in strain BL21(DE3)
-
gene pfl, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, overview
gene pfl, DNA and amino acid sequence determination and analysis, sequence comparison
-
gene pflB, coexpression of pyruvate formate lyase with the pyruvate formate lyase activating enzyme (gene pflA, UniProt ID P0A9N4) as well as with appropriate electron donors flavodoxin and ferredoxin (encoded by genes fldA and fdx, respectively), in a non-ethanol-producing Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc- MTH1-DELTAT ura3-52), plasmid maps, overview. Reduced flavodoxin is the preferred electron donor, but coexpression of either of the electron donors has a positive effect on growth under aerobic conditions. Subcloning in Escherichia coli strain DH5alpha. The PFL pathway can be functional at aerobic growth conditions in yeast when coexpressed with appropriate electron donors
gene pflB, enzyme expression analysis
-
gene pflB, expression analysis
-
gene pflB, ligands CcpA, GlnR, and GntR interact with the putative promoters of pflA and pflB, CcpA and GlnR bind to promoters containing cre sites
gene pflB, overexpression of the enzyme from plasmid 29 in the soluble fraction of Escherichia coli strain MC4100
gene pflB, the enzyme and its activating enzyme from Escherichia coli are expressed in a Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1. Enzyme PFL is expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively. The coexpression of either of these electron donors has a positive effect on growth under aerobic conditions, indicating increased activity of PFL.
overexpression in Escherichia coli
-
overexpression of the His6-tagged enzyme as single enzyme or as AdhE-Pfl in strain BL21(DE3)
-
overproducing of the enzyme in Escherichia coli 234M1 transformed with expression vector p153E1
PCR-amplificatioin, mariner plasmid
-
pfl gene cloned and expressed in Escherichia coli HB101
-
pfl gene expressed under control of different constitutive promoters, PFL-deficient strain CRM40 complemented
-
recombinant PFL expressed in Escherichia coli BL21 (DE3)
recombinant pfl in Escherichia coli RM220
-
recombinant pfl in Escherichia coli RM221
structural gene pfl cloned and sequenced, homologous expression and overproduction in Escherichia coli K12
-