1.14.13.231: tetracycline 11a-monooxygenase
This is an abbreviated version!
For detailed information about tetracycline 11a-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.231
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1.14.13.231
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electrocatalytic
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bacteroides
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greenhouse
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electrochemical
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enzyme-based
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polyethyleneimine
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glassy
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biosensing
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electrode
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biosensors
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thetaiotaomicron
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dissipation
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metagenomic
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degradation
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biotechnology
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analysis
- 1.14.13.231
-
electrocatalytic
-
bacteroides
-
greenhouse
-
electrochemical
-
enzyme-based
-
polyethyleneimine
-
glassy
-
biosensing
-
electrode
-
biosensors
- thetaiotaomicron
-
dissipation
-
metagenomic
- degradation
- biotechnology
- analysis
Reaction
Synonyms
BN1088_100004, tetracycline resistance protein from transposon Tn4351/Tn4400, tetracylcine resistance protein TetX, TetX, TetX family tetracycline inactivation enzyme, TetX2
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Application
Application on EC 1.14.13.231 - tetracycline 11a-monooxygenase
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analysis
biotechnology
degradation
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construction of a sensitive fluorescent reporter system to detect and characterize the activity of enzymes that act upon the antibiotic, tetracycline and its derivatives. In this system, expression of the lux operon is regulated by the tetracycline repressor, TetR, which is expressed from the same plasmid under the control of an arabinose-inducible promoter. Addition of very low concentrations of tetracycline derivatives, well below growth inhibitory concentrations, result in luminescence production. Introduction of a plasmid expressing TetX, a tetracycline-inactivating enzyme, causes a marked loss in luminescence due to enzyme-mediated reduction in the intracellular tetracycline concentration
analysis
construction of an enzyme-based biosensor for sensitive determination of tetracycline using TetX2 immobilized on modified glassy carbon electrodes. The electrode as a biosensing interface based on the oxygen reduction peak current resuls in a linear range response from 0.5 to 5 microM with a detection limit of 18 nM
protocol for the nuclear transformation of Chlamydomonas reinhardtii using tetX as a selectable marker that confers stable resistance to tetracycline up to 100 microg/ml. TetX may be used to transform Chlamydomonas reinhardtii chloroplasts, related microalgae and other aerobic organisms sensitive to any tetracycline antibiotic
biotechnology
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protocol for the nuclear transformation of Chlamydomonas reinhardtii using tetX as a selectable marker that confers stable resistance to tetracycline up to 100 microg/ml. TetX may be used to transform Chlamydomonas reinhardtii chloroplasts, related microalgae and other aerobic organisms sensitive to any tetracycline antibiotic
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addition of Escherichia coli overexpressing TetX to soil bacterial enrichment cultures along with varying levels of tetracycline affects community-wide tetracycline resistance levels. Soil microbial communities develop lower levels of tetracycline resistance upon exposure to 25 microg/ml of tetracycline when an Escherichia coli expressing TetX is present (6% of cultivable bacteria are resistant to 40 microg/ml tetracycline). In the absence of TetX activity, a similar tetracycline exposure selects for greater levels of resistant bacteria in the soil microbial community (90% of cultivable bacteria are resistant to 40 microg/ml tetracycline)
degradation
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addition of Escherichia coli overexpressing TetX to soil bacterial enrichment cultures along with varying levels of tetracycline affects community-wide tetracycline resistance levels. Soil microbial communities develop lower levels of tetracycline resistance upon exposure to 25 microg/ml of tetracycline when an Escherichia coli expressing TetX is present (6% of cultivable bacteria are resistant to 40 microg/ml tetracycline). In the absence of TetX activity, a similar tetracycline exposure selects for greater levels of resistant bacteria in the soil microbial community (90% of cultivable bacteria are resistant to 40 microg/ml tetracycline)
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