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1.14.13.225: F-actin monooxygenase

This is an abbreviated version!
For detailed information about F-actin monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.225

Reaction

[F-actin]-L-methionine
+
NADPH
 +
O2
 +
H+
=
[F-actin]-L-methionine-(R)-S-oxide
 +
NADP+
 +
H2O

Synonyms

MICAL, MICAL-1, MICAL-2, MICAL1, Mical2, MICAL2PV, MICAL3

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.225 F-actin monooxygenase

Application

Application on EC 1.14.13.225 - F-actin monooxygenase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
elevated MICAL1 gene expression is observed in invasive breast cancer samples from human patients relative to normal tissue, while MICAL1 amplification or point mutations are associated with reduced progression free survival
synthesis
-
protocol for obtaining high levels of recombinant protein for the redox only portion of Mical. Cold adapted chaperonins alone do not work well for expressing the Mical redox domain, while the use and removal of solubility tags destabilizes. Low-temperature expression, chaperonins and no solubility tag lead to enhanced expression