EC Number   |
Protein Variants |
Reference |
|---|
   4.6.1.25 | D51G |
mutant form loses toxicity when expressed in Escherichia coli from standard expression vectors |
754077 |
| 4.6.1.25 | E105A |
mutant form loses toxicity when expressed in Escherichia coli from standard expression vectors |
754077 |
| 4.6.1.25 | E19A |
mutation lowers toxicity without completely abolishing it when expressed in Escherichia coli from standard expression vectors |
754077 |
| 4.6.1.25 | E19V |
mutation lowers toxicity without completely abolishing it when expressed in Escherichia coli from standard expression vectors |
754077 |
| 4.6.1.25 | E21V |
mutation has no influence on toxicity when expressed in Escherichia coli from standard expression vectors |
754077 |
| 4.6.1.25 | H42A |
the mutant enzyme cleaves the substrate with a rate similar to that of the wild-type enzyme |
754920 |
| 4.6.1.25 | H48A |
mutant enzyme binds its RNA substrate with the same affinity as the wild type protein. The beta-sheet content of the RegB H48A secondary structure is probably poor but not nil |
758282 |
| 4.6.1.25 | H48A |
mutation completely abolishes activity without changing its ability to bind the substrate or affecting its overall structure |
754920 |
| 4.6.1.25 | H68A |
mutation significantly reduces RegB activity without changing its ability to bind the substrate or affecting its overall structure |
754920 |
| 4.6.1.25 | H73A |
the mutant enzyme cleaves the substrate with a rate similar to that of the wild-type enzyme |
754920 |
