Literature summary for 4.6.1.25 extracted from

Saida, F.; Odaert, B.; Uzan, M.; Bontems, F.
First structural investigation of the restriction ribonuclease RegB NMR spectroscopic conditions, 13C/15N double-isotopic labelling and two-dimensional heteronuclear spectra (2004), Protein Expr. Purif., 34, 158-165 .

Search for more literature for 4.6.1.25

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Tequatrovirus T4

Protein Variants

Protein Variants	Comment	Organism
H48A	mutant enzyme binds its RNA substrate with the same affinity as the wild type protein. The beta-sheet content of the RegB H48A secondary structure is probably poor but not nil	Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a [pre-mRNA]-containing guanosine-adenosine + H2O	Tequatrovirus T4	-	a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	P13312	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Tequatrovirus T4

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a [pre-mRNA]-containing guanosine-adenosine + H2O	-	Tequatrovirus T4	a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate	-	?
a [pre-mRNA]-containing guanosine-adenosine + H2O	the enzyme cleaves with an almost absolute specificity its RNA substrate in the middle of the GGAG tetranucleotide mainly found in the Shine-Dalgarno sequence	Tequatrovirus T4	a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
RegB	-	Tequatrovirus T4

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
32	-	1.2 mM samples of RegB H48A precipitate at 32.5°C within 18 h. In the presence of sodium citrate molecules no visible precipitation after a 3 days-incubation	Tequatrovirus T4

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)