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Information on EC 4.6.1.25 - bacteriophage T4 restriction endoribonuclease RegB

for references in articles please use BRENDA:EC4.6.1.25
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EC Tree
IUBMB Comments
The enzyme from bacteriophage T4 cleaves early mRNAs between Ap and Gp at one specific specific GpGpApGp site, favouring their further transition to middle-phase mRNA. The activity is enhanced by Ribosomal S1 protein. The enzyme catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending with 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
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Word Map
  • 4.6.1.25
  • shine-dalgarno
  • rnase
  • intergenic
  • tetranucleotide
  • sequence-specific
The enzyme appears in viruses and cellular organisms
Reaction Schemes
hide(Overall reactions are displayed. Show all >>)
a [pre-mRNA]-containing guanosine-adenosine
+
=
a 5' hydroxy-guanosine-[pre-mRNA fragment]
+
a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
Synonyms
endoribonuclease regb, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a [pre-mRNA fragment]- adenosine-2',3'-cyclophosphate + H2O = a [pre-mRNAfragment]-3'-adenosine-3'-phosphate
show the reaction diagram
(1b)
-
-
-
a [pre-mRNA]-containing guanosine-adenosine + H2O = a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
show the reaction diagram
overall reaction
-
-
-
a [pre-mRNA]-containing guanosine-adenosine + H2O = a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-adenosine-2',3'-cyclophosphate
show the reaction diagram
(1a)
-
-
-
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