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Literature summary for 4.6.1.25 extracted from

  • Odaert, B.; Saida, F.; Aliprandi, P.; Durand, S.; Crechet, J.B.; Guerois, R.; Laalami, S.; Uzan, M.; Bontems, F.
    Structural and functional studies of RegB, a new member of a family of sequence-specific ribonucleases involved in mRNA inactivation on the ribosome (2007), J. Biol. Chem., 282, 2019-2028 .
    View publication on PubMed
Search for more literature for 4.6.1.25

Activating Compound

Activating Compound Comment Organism Structure
ribosomal 30 S subunit the activity of the enzyme (RegB) is very low but can be enhanced up to 100fold by the ribosomal 30 S subunit or by ribosomal protein S1 Tequatrovirus T4
ribosomal protein S1 the activity of the enzyme (RegB) is very low but can be enhanced up to 100fold by the ribosomal 30 S subunit or by ribosomal protein S1 Tequatrovirus T4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3)-pLysS, transformed with plasmid pEH48A. RegB is highly toxic to Escherichia coli, preventing its overproduction from standard expression vectors Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
D51G mutant form loses toxicity when expressed in Escherichia coli from standard expression vectors Tequatrovirus T4
E105A mutant form loses toxicity when expressed in Escherichia coli from standard expression vectors Tequatrovirus T4
E19A mutation lowers toxicity without completely abolishing it when expressed in Escherichia coli from standard expression vectors Tequatrovirus T4
E19V mutation lowers toxicity without completely abolishing it when expressed in Escherichia coli from standard expression vectors Tequatrovirus T4
E21V mutation has no influence on toxicity when expressed in Escherichia coli from standard expression vectors Tequatrovirus T4
R11Q mutant form loses toxicity when expressed in Escherichia coli from standard expression vectors Tequatrovirus T4
R56L mutant form loses toxicity when expressed in Escherichia coli from standard expression vectors Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [pre-mRNA]-containing guanosine-adenosine + H2O Tequatrovirus T4 the enzyme participates in the bacteriophage T4 life cycle by favoring early messenger RNA breakdown. It specifically cleaves GGAG sequences found in intergenic regions, mainly in translation initiation sites a 5'-hydroxy-guanosine-[premRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P13312
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [pre-mRNA]-containing guanosine-adenosine + H2O the enzyme participates in the bacteriophage T4 life cycle by favoring early messenger RNA breakdown. It specifically cleaves GGAG sequences found in intergenic regions, mainly in translation initiation sites Tequatrovirus T4 a 5'-hydroxy-guanosine-[premRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
 ?
a [pre-mRNA]-containing guanosine-adenosine + H2O the enzyme specifically cleaves GGAG sequences found in intergenic regions, mainly in translation initiation sites Tequatrovirus T4 a 5'-hydroxy-guanosine-[premRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
RegB
-
 Tequatrovirus T4

General Information

General Information Comment Organism
physiological function the enzyme participates in the bacteriophage T4 life cycle by favoring early messenger RNA breakdown Tequatrovirus T4