EC Number   |
Protein Variants |
Reference |
|---|
   4.2.99.B1 | D256A |
site-directed mutagenesis, C-terminal polymerase domain, completely eliminates dRP lyase activity, proficient in DNA synthesis by the polymerase reaction |
694332 |
| 4.2.99.B1 | D829N/E830Q |
the polymerase inactive mutant D829N/E830Q retains full 5'-dRP lyase activity. Domain mapping of the 98-kDa enzyme reveals that the 5'-dRP lyase active site resides in a 24-kDa-domain |
694467 |
| 4.2.99.B1 | E71Q |
retains wild-type enzyme activity |
692940 |
| 4.2.99.B1 | E75A |
about 75% of wild-type enzyme activity |
692940 |
| 4.2.99.B1 | F25W |
about 85% of wild-type enzyme activity |
692940 |
| 4.2.99.B1 | H34G |
about 45% of wild-type enzyme activity |
692940 |
| 4.2.99.B1 | K310A |
eliminates more than 90% of the wild-type dRP lyase activity, indicating that Lys 310 is the main nucleophile involved in the reaction, forming the Schiff base internediate during beta-elimination |
692957 |
| 4.2.99.B1 | K35A |
about 50% of wild-type enzyme activity, Lys72 and Lys35 are involved in the dRP lyase reaction catalyzed by the 8-kDa domain and Lys35 is involved in 5'-phosphate recognition. Lys35 may stabilize the leaving group in the dRP lyase reaction through its interaction with the 5'-phosphate of the DNA-terminus |
692940 |
| 4.2.99.B1 | K35A |
part of the active site, 50% loss of enzyme activity |
692010 |
| 4.2.99.B1 | K35A/K68A |
part of the active site, similar to wild-type enzyme activity |
692010 |
