Literature summary for 4.2.99.B1 extracted from

Prasad, R.; Beard, W.A.; Chyan, J.Y.; Maciejewski, M.W.; Mullen, G.P.; Wilson, S.H.
Functional analysis of the amino-terminal 8-kDa domain of DNA polymerase beta as revealed by site-directed mutagenesis. DNA binding and 5'-deoxyribose phosphate lyase activities (1998), J. Biol. Chem., 273, 11121-11126.

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Cloned(Commentary)

Cloned (Comment)	Organism
the mutants K72A, K68A/K72A, K35A, K60A, H34G, E75A, F25W, K68A, K84A, E71Q, and K68A/E71Q are expressed in Escherichia coli, and the recombinant proteins are purified to near homogeneity, to 95%. Mutant proteins show similar behaviour to wild-type protein, indicating less changes in structural properties	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E71Q	retains wild-type enzyme activity	Homo sapiens
E75A	about 75% of wild-type enzyme activity	Homo sapiens
F25W	about 85% of wild-type enzyme activity	Homo sapiens
H34G	about 45% of wild-type enzyme activity	Homo sapiens
K35A	about 50% of wild-type enzyme activity, Lys72 and Lys35 are involved in the dRP lyase reaction catalyzed by the 8-kDa domain and Lys35 is involved in 5'-phosphate recognition. Lys35 may stabilize the leaving group in the dRP lyase reaction through its interaction with the 5'-phosphate of the DNA-terminus	Homo sapiens
K35A/K68A/K72A	devoid of dRP lyase, Lys72 and Lys35 are involved in the dRP lyase reaction catalyzed by the 8-kDa domain and Lys35 is involved in 5'-phosphate recognition. Lys35 may stabilize the leaving group in the dRP lyase reaction through its interaction with the 5'-phosphate of the DNA-terminus	Homo sapiens
K60A	about 40% of wild-type enzyme activity	Homo sapiens
K68A	retains wild-type enzyme activity	Homo sapiens
K68A/E71Q	retains wild-type enzyme activity	Homo sapiens
K68A/K72A	about 10% of wild-type enzyme activity	Homo sapiens
K72A	less than 10% of wild-type enzyme activity, Lys72 and Lys35 are involved in the dRP lyase reaction catalyzed by the 8-kDa domain and Lys35 is involved in 5'-phosphate recognition. Lys35 may stabilize the leaving group in the dRP lyase reaction through its interaction with the 5'-phosphate of the DNA-terminus	Homo sapiens
K84A	retains wild-type enzyme activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	8-kDa domain dRP lyase in the crude cell extract	Homo sapiens	-	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P06746	-	-

Purification (Commentary)

Purification (Comment)	Organism
the mutants K72A, K68A/K72A, K35A, K60A, H34G, E75A, F25W, K68A, K84A, E71Q, K35A/K68A/K72A, and K68A/E71Q are expressed in Escherichia coli, and the recombinant proteins are purified to near homogeneity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
49-residue oligonucleotide duplex DNA	substrate contains uracil residue at position 21. The DNA is pretreated with uracil DNA glycosylase and AP endonuclease	Homo sapiens	? + 2-deoxy-D-ribose 5-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
5'-deoxyribose phosphate lyase	-	Homo sapiens
dRP lyase	-	Homo sapiens

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Release 2023.1 (January 2023)