Literature summary for 4.2.99.B1 extracted from

Garcia-Diaz, M.; Bebenek, K.; Kunkel, T.A.; Blanco, L.
Identification of an intrinsic 5'-deoxyribose-5-phosphate lyase activity in human DNA polynmerase lambda. A possible role in base excision repair (2001), J. Biol. Chem., 276, 34659-34663.

Search for more literature for 4.2.99.B1

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant pol lambda and the K310A mutant are overexpressed in Eschercihia coli and purified to near homogeneity	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K310A	eliminates more than 90% of the wild-type dRP lyase activity, indicating that Lys 310 is the main nucleophile involved in the reaction, forming the Schiff base internediate during beta-elimination	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UGP5	the 8-kDa-domain of pol lambda, residues 240-325 show high amino acid similarity (30%) with that of pol beta, catalyzing the same reaction, residues 1-87, UniProt-ID: P06746	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant pol lambda and the K310A mutant are overexpressed in Eschercihia coli and purified to near homogeneity	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site	homologous to the dRP lyase activity of polymerase beta, pol beta. Proposed to participate in single-nucleotide base excision repair in mammalian cells, which is the major repair pathway in eukaryotic cells responsible for repair of lesions that give rise to abasic siles in DNA	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
34-bp oligonucleotide containing uracil at position 16	labelled with 3'-end by terminal deoxynucleotidyltransferase using [alpha-32P]ddATP and annealed to its complementary oligonucleotide, and pretreated with human uracil-DNA glycosylase and AP endonuclease	Homo sapiens	? + 2-deoxy-D-ribose 5-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
5'-deoxyribose-5-phosphate lyase	-	Homo sapiens
DNA polymerase lambda	dRP lyase activity	Homo sapiens
dRP lyase	-	Homo sapiens
Pol lambda	-	Homo sapiens

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Release 2023.1 (January 2023)