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Literature summary for 4.2.99.B1 extracted from

  • Prasad, R.; Batra, V.K.; Yang, X.P.; Krahn, J.M.; Pedersen, L.C.; Beard, W.A.; Wilson, S.H.
    Structural insight into the DNA polymerase beta deoxyribose phosphate lyase mechanism (2005), DNA Repair, 4, 1347-1357.
    View publication on PubMed
Search for more literature for 4.2.99.B1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant wild-type and mutant proteins H34G, K35A, K35R, Y39F, K68A, K68R, K72A, K72R, K35A/K68A, K35A/K72A, K35A/K68A/K72A, K35R/K68R/K72R are expressed in Escherichia coli and purified Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization by sitting-drop vapor diffusion. Diffraction data are obtained for crystals of Pol beta/nicked DNA complexes containing a sugar in either a closed-ring or open form, with resolution limits 2.4-2.6 A Homo sapiens

Protein Variants

Protein Variants Comment Organism
K35A part of the active site, 50% loss of enzyme activity Homo sapiens
K35A/K68A part of the active site, similar to wild-type enzyme activity Homo sapiens
K35A/K68A/K72A part of the active site, above 95% loss of enzyme activity Homo sapiens
K35A/K72A part of the active site, above 95% loss of enzyme activity Homo sapiens
K35R part of the active site, no effect on enzyme activity Homo sapiens
K35R/K68R/K72R part of the active site, above 95% loss of enzyme activity Homo sapiens
K68A part of the active site, stimulation of enzyme activity Homo sapiens
K68R part of the active site, significant reduction of enzyme activity Homo sapiens
K72A part of the active site, above 95% loss of enzyme activity Homo sapiens
K72R part of the active site, above 95% loss of enzyme activity Homo sapiens
Y39F part of the active site, similar to wild-type enzyme activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P06746
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant proteins H34G, K35A, K35R, Y39F, K68A, K68R, K72A, K72R, K35A/K68A, K35A/K72A, K35A/K68A/K72A, K35R/K68R/K72R are expressed in Escherichia coli and purified Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
labeled uracil-containing DNA [32P]-labeled, treated with with uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease Homo sapiens ? + 2-deoxy-D-ribose 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase beta exhibits deoxyribose phosphate lyase activity Homo sapiens
dRP lyase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens