Cloned (Comment) | Organism |
---|---|
expressed in MB36.3 cell, wild-type, SV40 T-Ag transformed cell line haboring the lambda phage transgene lambdaLIZ | Mus musculus |
MB38DELTA4, beta-pol null, sensitive to the methylating agent methylmethanesulfonate, SV40 T-Ag transformed cell line haboring the lambda phage transgene lambdaLIZ | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D256A | site-directed mutagenesis, C-terminal polymerase domain, completely eliminates dRP lyase activity, proficient in DNA synthesis by the polymerase reaction | Mus musculus |
K35A/K68A/K72A | site-directed mutagenesis, amino-terminal dRP lyase domain, completely eliminates dRP lyase activity, proficient in DNA synthesis by the polymerase reaction | Mus musculus |
K35A/K68A/K72A/D256A | site-directed mutagenesis, amino-terminal dRP lyase domain, deficient in dRP lyase and polymerase activity | Mus musculus |
additional information | an active-site deletion mutant of beta-pol, in which residues within the C-terminal portion, residues 263-335 that are required for DNA synthesis are deleted, completely deficient in single-nucleotide base excision repair DNA synthesis, but active in 5'-deoxyribose phosphate removal | Mus musculus |
R283A | site-directed mutagenesis, C-terminal polymerase domain, deficient in base excision repair DNA synthesis activity of the polymerase, but has full dRP lyase activity | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | removal of the 5'-deoxyribose phosphate group is a pivotal step in the base excision repair (BER) pathway in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site | removal of the 5'-deoxyribose phosphate group is a pivotal step in the base excision repair (BER) pathway in vivo.Two important enzymatic steps in mammalian single-nucleotide base excision repair are contributed by beta-pol: DNA resynthesis of the repair patch und lyase removal of 5'-deoxyribose phosphate | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
50-bp DNA containing a single abasic site preincised with AP endonuclease | - |
Mus musculus | 29-bp DNA fragment + 2-deoxy-D-ribose 5-phosphate | - |
? | |
additional information | removal of the 5'-deoxyribose phosphate group is a pivotal step in the base excision repair (BER) pathway in vivo | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-pol | - |
Mus musculus |
DNA beta-polymerase | lyase activity | Mus musculus |
dRP lyase | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |