EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.1.8.1 | -999 |
- |
106°C is the melting temperature. The temperatures required to lose one half of enzymatic activity in 5 minutes are 92°C and 130°C in liquid and solid states, respectively. More than 30% activity remains after autoclaving the enzyme powder at 121°C for 15 min |
752208 |
3.1.8.1 | -999 |
- |
highly thermostable enzyme |
719426 |
3.1.8.1 | -999 |
- |
human PON1 exhibits intrinsically a remarkable thermal stability. The association of human phosphate binding protein, though it does not change the denaturation temperature, strongly contributes to slow the rate of irreversible denaturation |
678464 |
3.1.8.1 | -999 |
- |
PON1 is highly thermostable, especially when associated to the human phosphate-binding protein |
678109 |
3.1.8.1 | -999 |
- |
rat plasma enzyme is more stable to heat inactivation than rat liver microsomal enzyme, no loss of activity in human serum after 3 days and 33% after 5 days |
646508 |
3.1.8.1 | 20 |
- |
plasma enzyme stable, liver enzyme less stable |
35209 |
3.1.8.1 | 25 |
90 |
with methyl paraoxon as a substrate, retains full activity after incubation for 15 min between 25-90°C. Can withstand an incubation temperature of 60°C for two days, and retains 72% of its activity after incubation at 90°C for 2 h. The melting temperature is 106.6°C |
707276 |
3.1.8.1 | 27 |
- |
24 h, 80% loss of activity of rat liver and plasma enzyme |
646513 |
3.1.8.1 | 30 |
60 |
the thermostability of hydrolyric activity of recombinant PON1-hFc is slightly increased compared to recombinant wild-type PON1, inactivation of PON1 at 55°C, inactivation of PON1-hFc at 60°C, profiles overview |
752102 |
3.1.8.1 | 37 |
- |
is stable for 8 to 10 h at 37°C. Completely loses activity at 50°C |
707214 |