EC Number   |
Application |
Reference |
|---|
    1.7.3.3 | analysis |
a colorimetric 96-well microtiter plate assay for the determination of urate oxidase activity and its kinetic parameters based on hydrogen peroxide quantitation. The general advantages of the colorimetric assay are easy handling of large amounts of samples at the same time, the possibility of automation, and the need for less material |
657529 |
| 1.7.3.3 | analysis |
development of a high-throughput screening system of Bacillus fastidiosus uricase mutants, using 96-well plates and monitoring of uric acid by absorbance at 298 nm |
741653 |
| 1.7.3.3 | analysis |
development of an urate-selective microbial biosensor cells of the recombinant thermotolerant methylotrophic yeast Hansenula polymorpha as biorecognition element. The UOX producing cells are coupled to horseradish peroxidase and immobilized on graphite electrodes by physical entrapment behind a dialysis membrane. A high urate selectivity with a detection limit of about 8 microM is found |
724661 |
| 1.7.3.3 | analysis |
modified colorimetric assay for uricase activity in flexible 96-well microtiter plates using the uricase/uric acid/horseradish peroxidase/4-aminoantipyrine/3,5-dichloro-2-hydroxybenzene sulfonate colorimetric reaction. The method is much more efficient than the conventional ones and greatly reduces assay time from 4 days to less than 20 h |
658651 |
| 1.7.3.3 | analysis |
specific activity calculated from the level of total proteins is a favorable index for recognizing an enzyme mutant with small improvement of activity. Uricase mutants whose activities are improved by more than 80% are recognized with higher sensitivity and specificity during screening a library of enzyme mutants expressed in Escherichia coli |
742262 |
| 1.7.3.3 | analysis |
uric acid can be detected in human sera with the enzyme with none of the tested uric acid analogs being a competitive substrate |
-, 743753 |
| 1.7.3.3 | drug development |
urate oxidase has the potential to be a therapeutic target for the treatment of gout |
701100 |
| 1.7.3.3 | medicine |
determination of the urate concentration in blood and urine is required for the diagnosis of gout as urate accumulation is a causative factor of gout in humans |
696795 |
| 1.7.3.3 | medicine |
determining the urate concentration in blood and urine |
-, 394181, 394182 |
| 1.7.3.3 | medicine |
oral uricase therapy significantly decreases plasma uric acid concentrations in pigs with chronic kidney disease |
765645 |
