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Literature summary for 1.7.3.3 extracted from

  • Fraisse, L.; Bonnet, M.C.; de Farcy, J.P.; Agut, C.; Dersigny, D.; Bayol, A.
    A colorimetric 96-well microtiter plate assay for the determination of urate oxidase activity and its kinetic parameters (2002), Anal. Biochem., 309, 173-179.
    View publication on PubMed

Application

Application Comment Organism
analysis a colorimetric 96-well microtiter plate assay for the determination of urate oxidase activity and its kinetic parameters based on hydrogen peroxide quantitation. The general advantages of the colorimetric assay are easy handling of large amounts of samples at the same time, the possibility of automation, and the need for less material Aspergillus flavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0472
-
Urate Rasburicase Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus Q00511 expressed in Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
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Aspergillus flavus 5-hydroxyisourate + H2O2
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?

Synonyms

Synonyms Comment Organism
ELITEK
-
Aspergillus flavus
Rasburicase
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Aspergillus flavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.1
-
Urate Rasburicase Aspergillus flavus