Any feedback?
Literature summary for 1.7.3.3 extracted from
- Site-specific incorporation of unnatural amino acids into urate oxidase in Escherichia coli (2008), Protein Sci., 17, 1827-1833.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | urate oxidase has the potential to be a therapeutic target for the treatment of gout | Cyberlindnera jadinii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| developing of a method for genetically incorporating p-azido-L-phenylalanine into target protein in Escherichia coli in a site-specific manner utilizing a tyrosyl suppressor tRNA/aminoacyl-tRNA synthetase system, substitution of p-azido-L-phenylalanine for F170 or F281 in urate oxidase, optimization of the system by adding a Shine-Dalgarno sequence and tandem suppressor tRNA in order to increase the expression levels of tyrosyl suppressor tRNA and aminoacyl-tRNA synthetase | Cyberlindnera jadinii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Cyberlindnera jadinii | purine degradation, urate oxidase catalyzes the oxidation of uric acid with poor solubility to produce 5-hydroxyisourate and allantoin | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-NTA affinity chromatography | Cyberlindnera jadinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.49 | - |
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 281, in 0.1 M borate, pH 8.4 | Cyberlindnera jadinii |
| 8.26 | - |
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 170, in 0.1 M borate, pH 8.4 | Cyberlindnera jadinii |
| 16.33 | - |
natural uricase, in 0.1 M borate, pH 8.4 | Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | purine degradation, urate oxidase catalyzes the oxidation of uric acid with poor solubility to produce 5-hydroxyisourate and allantoin | Cyberlindnera jadinii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Urate oxidase | - |
Cyberlindnera jadinii |
| uricase | - |
Cyberlindnera jadinii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cyberlindnera jadinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
assay at | Cyberlindnera jadinii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
